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MASTL Antibody

  • 货号：
    CSB-PA846629LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, Raji whole cell lysate, U87 whole cell lysate
      All lanes: MASTL antibody at 6.3µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 98, 93 kDa
      Observed band size: 98 kDa
    • IHC image of CSB-PA846629LA01HU diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA846629LA01HU diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) MASTL Polyclonal antibody
  • Uniprot No.：
    Q96GX5
  • 基因名：
    MASTL
  • 别名：
    2700091H24Rik antibody; C88295 antibody; FLJ14813 antibody; GREATWALL antibody; Greatwall protein kinase antibody; GW antibody; GWL antibody; GWL_HUMAN antibody; hGWL antibody; MAST-L antibody; Mastl antibody; MGC117975 antibody; Microtubule associated serine/threonine kinase like antibody; Microtubule-associated serine/threonine-protein kinase-like antibody; RP11 85G18.2 antibody; Serine/threonine-protein kinase greatwall antibody; THC2 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Serine/threonine-protein kinase greatwall protein (612-740AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，MASTL Antibody (CSB-PA846629LA01HU)，的标记方式是Non-conjugated。对于MASTL Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA846629LB01HU MASTL Antibody, HRP conjugated ELISA
    FITC CSB-PA846629LC01HU MASTL Antibody, FITC conjugated
    Biotin CSB-PA846629LD01HU MASTL Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.
  • 基因功能参考文献：
    1. Using mathematical modelling, this paper confirms that deactivation of MASTL is essential for mitotic exit. PMID: 26872783
    2. these results established that precise control of MASTL is essential to couple DNA damage to mitosis through the rate of mitotic entry and APC/C activation. PMID: 26923777
    3. Thus, GWL is a human oncoprotein that promotes the hyperactivation of AKT via the degradation of its phosphatase, PHLPP, in human malignancies. PMID: 26613407
    4. Thus, Fcp1 coordinates Cdk1 and Gwl inactivation to derepress PP2A-B55, generating a dephosphorylation switch that drives mitosis progression. PMID: 26653855
    5. Boolean modeling identifies Greatwall/MASTL as an important regulator in the AURKA network of neuroblastoma. PMID: 26616283
    6. Data show that siRNA knockdown of Forkhead box M1 (FOXM1) or microtubule-associated serine/threonine kinase-like (MASTL) induces radiosensitivity in non-small cell lung cancer (NSCLC). PMID: 25808837
    7. Mastl upregulation is involved in cancer progression and tumor recurrence after initial cancer therapy PMID: 25373736
    8. data demonstrate that GWL acts in a pathway with PP2A which is essential for prophase I exit and metaphase I microtubule assembly in mouse oocytes. PMID: 25472593
    9. Taken together our results suggest a hierarchy of phosphatases coordinating Greatwall, Ensa/ARPP19 and Cdk substrate dephosphorylation during mitotic exit. PMID: 24391510
    10. Studies indicate that mutations in three different genes within the THC2 locus have been associated with congenital thrombocytopenia, including a mutation in MASTL. PMID: 22102272
    11. results identify Gwl as a member of the AGC family of kinases that appears to be regulated by unique mechanisms and that differs from the other members of this family PMID: 21444715
    12. MASTL enhances cyclin B1-Cdk1-dependent mitotic phosphorylation events, directing mitotic entry, anaphase and cytokinesis in human cells. PMID: 20818157
    13. A novel missense mutation in the human gene FLJ14813 is associated with autosomal dominant thrombocytopenia PMID: 12890928
    14. A paper that narrows the identity of the gene for autosomal dominant thrombocytopenia (THC2) to FLJ14813. The mutation is present in all affected people across three generations while is absent in unaffected family members & 94 random blood donors. PMID: 12890928

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  • 相关疾病：
    Defects in MASTL may play a role in the pathogenesis of thrombocytopenia, a disorder defined by reduced number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Cleavage furrow.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family
  • 数据库链接：

    HGNC: 19042

    OMIM: 608221

    KEGG: hsa:84930

    STRING: 9606.ENSP00000365107

    UniGene: Hs.276905