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  4. MCM10 Antibody

MCM10 Antibody

  • 货号：
    CSB-PA013589GA01HU
  • 规格：
    ￥3,900
  • 其他：

产品详情

  • Uniprot No.：
    Q7L590
  • 基因名：
    MCM10
  • 别名：
    CNA43 antibody; DNA43 antibody; FLJ31133 antibody; Homolog of yeast MCM10 antibody; HsMCM10 antibody; mcm10 antibody; MCM10 minichromosome maintenance deficient 10 antibody; MCM10_HUMAN antibody; Minichromosome maintenance 10 replication initiation factor antibody; Minichromosome maintenance complex component 10 antibody; Minichromosome maintenance protein 10 isoform 1 antibody; PRO2249 antibody; Protein MCM10 homolog antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Human MCM10
  • 免疫原种属：
    Homo sapiens (Human)
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability; acts as a direct target of transcriptional repression by ZBTB38.
  • 基因功能参考文献：
    1. Results show that MCM10 is significantly upregulated in urothelial carcinoma (UC), and associated with tumor aggressiveness. Its knockdown significantly suppressed cell proliferation in UC cell lines. PMID: 27780919
    2. Data suggest that interaction of Mcm10 with Mcm2-7 multimer requires Mcm10 domain that contains amino acids 530-655, which overlaps with domain required for stable retention of Mcm10 on chromatin; Mcm10 conserved domain (amino acids 200-482) is essential for DNA replication; both conserved domain and Mcm2-7-binding domain are required for full activity of Mcm10. PMID: 28646110
    3. RecQL4-dependent association of Mcm10 and Ctf4 with replication origins appears to be the first important step controlled by S phase promoting kinases and checkpoint pathways for the initiation of DNA replication in human cells. PMID: 25602958
    4. MCM10 is the natural substrate of the Cul4-DDB1[VprBP] E3 ubiquitin ligase whose degradation is regulated by VprBP, but Vpr enhances the proteasomal degradation of MCM10 by interacting with VprBP. PMID: 26032416
    5. Loss of Mcm10 engages checkpoint, DNA repair and SUMO-dependent rescue pathways that collectively counteract replication stress and chromosome breakage. [Review] PMID: 24662891
    6. Mcm10 utilizes a modular architecture to act as a replisome scaffold, which helps to define possible roles in origin DNA melting, Pol alpha recruitment and coordination of enzymatic activities during elongation. PMID: 22918587
    7. Data suggest that CDC45 and MCM10 (minichromosome maintenance complex component 10) directly interact and establish a mutual co-operation in DNA binding; key domains appear to interact and then interact with DNA inside cells or in cell-free systems. PMID: 23750504
    8. This report shows that human Mcm10 is an acetylated protein regulated by SIRT1, which binds and deacetylates Mcm10 both in vivo and in vitro, and modulates Mcm10 stability and ability to bind DNA. PMID: 23449222
    9. High doses of ionizing gamma radiation and exposure to a combination of DNA-damaging chemicals do not decrease Mcm10 protein levels, demonstrating that Mcm10 down-regulation is triggered only by UV-specific damage PMID: 20064936
    10. human Mcm10 is temporarily recruited to the replication sites 30-60 min before they replicate and dissociates from chromatin after the activation of the prereplication complex PMID: 15136575
    11. transcription of human MCM10 and TopBP1 is activated by transcription factors E2F1-3, but not by factors E2F4-7 PMID: 15195143
    12. These results argue that cells can tolerate low levels of p180 as long as Mcm10 is present to "recycle" it. PMID: 17699597
    13. Mcm10 is required for chromatin loading of And-1. PMID: 17761813
    14. Results show that MCM10 molecule is a ring-shaped hexamer with large central and smaller lateral channels and a system of inner chambers. PMID: 17823614
    15. MCM10 is essential for the efficient elongation step of chromosome replication. PMID: 17997977
    16. These results indicate that MCM10 protein is essential for maintaining genome integrity as well as cell cycle progression. PMID: 17997981
    17. DNA and p180 binding to an Mcm10 construct that contains both the ID and CTD, provide the first mechanistic insight into how Mcm10 might use a handoff mechanism to load and stabilize pol alpha within the replication fork. PMID: 19608746
    18. MCM10 interacts directly with RECQ4 and regulates its DNA unwinding activity. PMID: 19696745
    19. Assembly of the Cdc45-Mcm2-7-GINS complex requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. PMID: 19805216
    20. Functional studies of the Drosophila homolog PMID: 12808023
    21. Functional studies of the Xenopus homolog PMID: 11864598

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  • 亚细胞定位：
    Nucleus. Note=Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli.
  • 蛋白家族：
    MCM10 family
  • 数据库链接：

    HGNC: 18043

    OMIM: 609357

    KEGG: hsa:55388

    STRING: 9606.ENSP00000354945

    UniGene: Hs.198363