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MED15 Antibody

  • 货号：
    CSB-PA842753LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: MCF-7 whole cell lysate
      All lanes: MED15 antibody at 5.2µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 87, 83, 76 kDa
      Observed band size: 87 kDa
    • Immunofluorescence staining of SH-SY5Y cells with CSB-PA842753LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) MED15 Polyclonal antibody
  • Uniprot No.：
    Q96RN5
  • 基因名：
    MED15
  • 别名：
    MED15 antibody; ARC105 antibody; CTG7A antibody; PCQAP antibody; TIG1 antibody; TNRC7 antibody; Mediator of RNA polymerase II transcription subunit 15 antibody; Activator-recruited cofactor 105 kDa component antibody; ARC105 antibody; CTG repeat protein 7a antibody; Mediator complex subunit 15 antibody; Positive cofactor 2 glutamine/Q-rich-associated protein antibody; PC2 glutamine/Q-rich-associated protein antibody; TPA-inducible gene 1 protein antibody; TIG-1 antibody; Trinucleotide repeat-containing gene 7 protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Mediator of RNA polymerase II transcription subunit 15 protein (433-530AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，MED15 Antibody (CSB-PA842753LA01HU)，的标记方式是Non-conjugated。对于MED15 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA842753LB01HU MED15 Antibody, HRP conjugated ELISA
    FITC CSB-PA842753LC01HU MED15 Antibody, FITC conjugated
    Biotin CSB-PA842753LD01HU MED15 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.
  • 基因功能参考文献：
    1. MED15 does seem to play a tumour promoting role in the progression and metastatic spread of renal cell carcinoma PMID: 29400661
    2. MED15 overexpression arises during androgen deprivation therapy via hyper-activation of PI3K/Akt/mTOR signaling pathway in prostate cancer cells. PMID: 27974704
    3. MED15 as a potential biomarker for head and neck squamous cell carcinoma PMID: 26457685
    4. MED15 is differentially expressed in tumor-free testis and testicular germ cell tumors PMID: 26377566
    5. MED15 overexpression is a clonal event during head and neck squamous cell carcinoma progression. PMID: 25791637
    6. these findings implicate MED15 in CRPC, and as MED15 is evolutionary conserved, it is likely to emerge as a lethal phenotype in other therapeutic-resistant diseases, and not restricted to our disease model. PMID: 24374838
    7. MED15 and PUM1 proteins with coiled-coil domains are potent enhancers of polyQ-mediated ataxin-1 protein misfolding and proteotoxicity in vitro and in vivo. PMID: 22916034
    8. The data identify PC2 as a novel PLAGL2-binding protein and important mediator of PLAGL2 transactivation. PMID: 20025940
    9. Methylation of TIG1 in prostatic neoplasms correlates with methylation of the retinoic acid receptor beta gene. PMID: 14691453
    10. Limited role of exon 7 PCQAP polymorphisms in the pathogenesis of schizophrenia. PMID: 15318033
    11. TIG1 gene Silencing by promoter hypermethylation is associated with nasopharyngeal carcinoma PMID: 15455391
    12. SREBPs use the evolutionarily conserved ARC105 (also called MED15) subunit to activate target genes PMID: 16799563
    13. results suggest that TRIM11, with the ubiquitin-proteasome pathway, regulates ARC105 function in TGFbeta signaling PMID: 16904669
    14. The protein encoded by this gene was identified as ARC105, a 105kDa component of the Activator-Recruited Cofactor (ARC) that is involved in mediating gene activation by several classes of transcriptional regulators. PMID: 10235267

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Mediator complex subunit 15 family
  • 组织特异性：
    Expressed in all tissues examined, including heart, brain, lung, spleen, thymus, pancreas, blood leukocyte and placenta. However, the level of expression varied, with highest expression in the placenta and peripheral blood and lowest in the pancreas and k
  • 数据库链接：

    HGNC: 14248

    OMIM: 607372

    KEGG: hsa:51586

    STRING: 9606.ENSP00000263205

    UniGene: Hs.517421