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MICAL1 Antibody

  • 货号：
    CSB-PA851555LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Raji whole cell lysate, Jurkat whole cell lysate, THP-1 whole cell lysate
      All lanes: MICAL1 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 118, 109, 38, 120 kDa
      Observed band size: 120 kDa
    • IHC image of CSB-PA851555LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA851555LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) MICAL1 Polyclonal antibody
  • Uniprot No.：
    Q8TDZ2
  • 基因名：
    MICAL1
  • 别名：
    CasL interacting molecule antibody; DKFZp434B1517 antibody; FLJ11937 antibody; FLJ21739 antibody; MICA1_HUMAN antibody; MICAL 1 antibody; MICAL antibody; MICAL1 antibody; microtubule associated monoxygenase; calponin and LIM domain containing 1 antibody; Molecule interacting with CasL protein 1 antibody; NEDD9 interacting protein with calponin homology and LIM domains antibody; NEDD9-interacting protein with calponin homology and LIM domains antibody; NICAL antibody; OTTHUMP00000016969 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human [F-actin]-methionine sulfoxide oxidase MICAL1 protein (806-1067AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，MICAL1 Antibody (CSB-PA851555LA01HU)，的标记方式是Non-conjugated。对于MICAL1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA851555LB01HU MICAL1 Antibody, HRP conjugated ELISA
    FITC CSB-PA851555LC01HU MICAL1 Antibody, FITC conjugated
    Biotin CSB-PA851555LD01HU MICAL1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels. May act as Rab effector protein and play a role in vesicle trafficking.
  • 基因功能参考文献：
    1. Methionine oxidation is regulated in vivo by monooxygenases of the MICAL family. (Review) PMID: 28229915
    2. describe methods to characterize MICAL-mediated F-actin disassembly using in vitro assays with purified proteins PMID: 27787846
    3. The results provide evidence that MICAL1 plays an essential role in the activation of ROS/Akt signaling and cell invasive phenotype and identify a novel link between RAB35 and MICAL1 in regulating breast cancer cell invasion. PMID: 27430308
    4. The MICAL1 directly induce oxidation of actin molecules, leading to actin depolymerization. ROS production by MICAL1 also causes oxidation of collapsin response mediator protein-2 which subsequently undergoes phosphorylation. PMID: 23834433
    5. although MICAL1 is auto-inhibited by its C-terminal coiled-coil region, MICAL2 remains constitutively active and affects stress fibers, suggesting differential but complementary roles for MICAL1 and MICAL2 in actin microfilament regulation PMID: 22331357
    6. Faint and scattered immunoreactivity for Mical-1 is observed in neurons of the neocortex of the temporal lobe epilepsy group, while strong immunoreactivity for Mical-1 is shown in control subjects. PMID: 21638339
    7. Findings show a biological role for MICAL-1 in apoptosis and define a novel negative regulatory mechanism of MST-NDR signaling. PMID: 21730291
    8. a CasL interacting molecule that associates with vimentin PMID: 11827972
    9. MICAL-1 isoforms with their multidomain structure are novel rab1 interacting proteins that function as scaffold proteins connecting different components in the cell. PMID: 12788069
    10. The unfolding of MICAL-1 calpolnin homology (CH) domain was studied. PMID: 17662518

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton. Midbody.
  • 蛋白家族：
    Mical family
  • 组织特异性：
    Expressed in the thymus, lung, spleen, kidney, testis and hematopoietic cells.
  • 数据库链接：

    HGNC: 20619

    OMIM: 607129

    KEGG: hsa:64780

    STRING: 9606.ENSP00000351664

    UniGene: Hs.33476