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MOCS3 Antibody

  • 货号：
    CSB-PA014707ESR1HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: MOCS3 antibody at 4.25μg/ml
      Lane 1: A549 whole cell lysate
      Lane 2: HepG2 whole cell lysate
      Lane 3: Jurkat whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 50 kDa
      Observed band size: 50 kDa
    • Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA014707ESR1HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA014707ESR1HU at dilution of 1:100
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) MOCS3 Polyclonal antibody
  • Uniprot No.：
    O95396
  • 基因名：
    MOCS3
  • 别名：
    Adenylyltransferase MOCS3 antibody; dJ914P20.3 antibody; MGC9252 antibody; MOCS 3 antibody; MOCS3 antibody; MOCS3_HUMAN antibody; Molybdenum cofactor synthesis 3 antibody; Molybdenum cofactor synthesis protein 3 antibody; Molybdopterin synthase sulfurylase antibody; Molybdopterin-synthase sulfurtransferase antibody; MPT synthase sulfurylase antibody; Sulfur carrier protein MOCS2A adenylyltransferase antibody; Sulfur carrier protein MOCS2A sulfurtransferase antibody; Sulfurtransferase MOCS3 antibody; UBA 4 antibody; UBA4 ubiquitin activating enzyme E1 homolog antibody; UBA4 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Adenylyltransferase and sulfurtransferase MOCS3 protein (271-460AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
  • 基因功能参考文献：
    1. This observation should encourage testing of additional intellectual disability patients with mild abnormalities of sulfite metabolism for MOCS3 mutations. PMID: 28544736
    2. Among the associated variants were two in regions previously unreported for COPD; a low frequency non-synonymous SNP in MOCS3 (rs7269297, pdiscovery=3.08x10(-6), preplication=0.019) and a rare SNP in IFIT3, which emerged in the meta-analysis (rs140549288, pmeta=8.56x10(-6)). PMID: 26917578
    3. ubiquitin-like Urm1.Uba4 systems are conserved and exchangeable between human and yeast cells PMID: 25747390
    4. extension of the C terminus with an additional glycine of MOCS2A and URM1 altered the localization of MOCS3 from the cytosol to the nucleus. PMID: 22453920
    5. MOCS3 protein is believed to catalyze both the adenylation and the subsequent generation of a thiocarboxylate group at the C terminus of the smaller subunit of molybdopterin synthase PMID: 15073332
    6. Electrospray ionization mass spectrometry performed on a rhodanese-like carboxyl-terminal domain of human MOCS3 provides direct evidence for the formation of persulfide on cysteine residue 412. PMID: 15910006
    7. in humans & most eukaryotes thiosulfate is not physiologic sulfur donor for MOCS3, whereas in bacterial homologs, which have arginine at last position of active site loop, thiosulfate can be used as a sulfur source for molybdenum cofactor biosynthesis. PMID: 17459099
    8. The UBA4-URM1 system represents the evolutionary link between protein conjugation and protein modification by sulfur carrier proteins. PMID: 18491921
    9. Nfs1 acts as a sulfur donor for MOCS3, a protein involved in molybdenum cofactor biosynthesis PMID: 18650437

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    HesA/MoeB/ThiF family, UBA4 subfamily
  • 数据库链接：

    HGNC: 15765

    OMIM: 609277

    KEGG: hsa:27304

    STRING: 9606.ENSP00000244051

    UniGene: Hs.159410