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MSH2 Antibody

  • 货号：
    CSB-PA791631
  • 规格：
    ￥2024
  • 图片：
    • Immunohistochemistry analysis of paraffin-embedded human brain tissue using MSH2 antibody.
    • Immunofluorescence analysis of HUVEC cells, using MSH2 antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) MSH2 Polyclonal antibody
  • Uniprot No.：
    P43246
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from internal of Human MSH2.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC,IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:200
    IF 1:100-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
  • 基因功能参考文献：
    1. Single-Nucleotide Polymorphisms of the MSH2 is associated with Basal Cell Carcinoma. PMID: 28667494
    2. MSH2-MSH3 not only stimulates pol beta to copy through the repeats but also enhances formation of the flap precursor for expansion. PMID: 27546332
    3. SNP Rs2303428 of MSH2 is associated with hepatocellular carcinoma prognosis in a Chinese population. PMID: 29874113
    4. An increased risk of breast cancer in MSH2 mutation carriers was demonstrated in a Canadian familial cancer registry. PMID: 28779004
    5. Loss of MSH2 protein is correlated with MSH2 inactivation, hypermutation, and higher tumor-infiltrating lymphocyte density, and appears most common among very high-grade primary tumors, for which routine screening may be warranted if validated in additional cohorts. PMID: 28790115
    6. Immunohistochemistry revealed loss of expression of proteins MSH2 and MSH6, strongly suggesting a diagnosis of MTS. PMID: 28256262
    7. s revealed a novel pathologic mutation on the MSH2 gene (G504 splicing) that associates with Lynch syndrome. Systematical comparison of the mutation landscape revealed that multiple cancers in the proband were evolutionarily independent. PMID: 28974240
    8. Expression of MSH6 and MSH2 was positively associated with tumor volume doubling time. Gene expression was positively associated with ATR expression. Reduction of MSH6 and MSH2 expression at the messenger RNA and protein levels could be involved in direct Pituitary Adenoma proliferation by promoting cell-cycle progression or decreasing the rate of apoptosis through interference with the function of the ATR-Chk1 pathway. PMID: 29342268
    9. Altered MSH2 expression detected in sporadic colon tumors pointing to its role in colorectal tumorigenesis PMID: 29715107
    10. MSH2 rs2303425 Polymorphism is Associated with Early-Onset Breast Cancer. PMID: 26975740
    11. Five out of twelve patients with defects in either of MSH2, RAD50 and NBN genes suffered from rare life-threatening AE, more frequently than in control group (p = 0.0005). When all detected variants were taken into account, the majority of patients (8 out of 15) suffered from life-threatening toxicity during chemotherapy. PMID: 28376765
    12. MSH2 inversion of exons 1-7 was found in four probands previously suspected to have Lynch syndrome based on family history and tumor testing PMID: 28004223
    13. Loss of MSH2 expression is associated with colorectal carcinoma. PMID: 28651545
    14. Findings indicate that carriers of the MSH5 rs707939 T allele, the MSH2 rs6544991 C allele, the MSH3 rs6151627 and rs6151670 G alleles, and the MSH3 rs7709909 T allele have poor toxicity tolerance to platinum-based chemotherapy in non-small cell lung cancer patients. PMID: 28093084
    15. MSH2 mutations contribute to colorectal cancer susceptibility in Algerian families with suspected Lynch syndrome. PMID: 27468915
    16. The data suggest that EZH2-H3K27me3 regulatory mechanism dynamically changes the expression levels of DNA mismatch repair gene MutS homolog 2, through epigenetic mark H3K27me3. PMID: 27036951
    17. In path_MSH2 10-year survival was 84%, relative incidence of subsequent cancer compared with incidence of first cancer was slightly but insignificantly higher than cancer incidence in patients with Lynch syndrome without previous cancer. PMID: 27261338
    18. Here we demonstrate that in silico saturation mutagenesis and biophysical calculations of the structural stability of the human mismatch repair protein MSH2 correlate with cellular protein levels, turnover and function. Of 24 different MSH2 variants, some of which are linked to Lynch syndrome, a destabilization of as little as 3 kcal/mol is sufficient to cause rapid degradation via the ubiquitin-proteasome pathway. PMID: 28422960
    19. human Pol alpha interacts with MSH2-MSH6 complex PMID: 27805738
    20. unlike MutSbeta, MutSalpha may also act to protect against repeat contractions in the Fmr1 gene PMID: 27427765
    21. A total of 201 unique disease-predisposing mismatch repair gene mutations were identified in 369 Lynch syndrome families. These mutations affected MLH1 in 40%, MSH2 in 36%, MSH6 in 18% and PMS2 in 6% of the families. PMID: 27601186
    22. There is a positive correlation between the expressions of hMSH2 and hMSH6 between males (RHO=0.673 and p=0.001) and females with colorectal adenocarcinoma. PMID: 27459116
    23. Individuals with Lynch syndrome and double-mutants in MSH6 and MSH2 had normal MSH2 expression, whereas MSH6 immunoexpression was lost in all evaluable cases. PMID: 26446363
    24. high expression of the MutSalpha complex was an independent prognostic factor for poor overall survival PMID: 27258499
    25. Upon stratifying the GEE model, reductions in ATM and MSH2 expression levels was heightened among women with an extended family history (FH) of breast cancer. Reduced expression of ATM and MSH2 compromises DNA repair capacity and, thereby, increases breast cancer prevalence. PMID: 27466510
    26. Alterations in p53 and p21 expression suggest that these proteins are involved in lower lip carcinogenesis. Moreover, p53 and hMSH2 seem to be interrelated in early events of this process. PMID: 26842232
    27. screen was able to distinguish weak and strong pathogenic variants from polymorphisms and was used to investigate 59 Msh2 VUS. Nineteen of the 59 VUS were identified as pathogenic PMID: 26951660
    28. Report a family with an MSH2 mutation c.1126_1127delTT (p.Leu376Thrfs*12). A 46-year-old male proband developed keratoacanthoma, colon cancer and gastric cancer, and fulfilled the diagnostic criteria for Muir-Torre syndrome. His 80-year-old mother, diagnosed with hereditary non-polyposis colorectal cancer, presented with multiple gastrointestinal tract cancers, Bowen's disease and actinic keratosis. PMID: 26077460
    29. In Lynch syndrome families, prostate cancer was associated with mutations in MSH2 with loss of the mismatch repair protein. PMID: 27013479
    30. Loss of MSH-2/MSH-6 expression was correlated with the right-colon location, poor and mucinous differentiation in colorectal adenocarcinoma. PMID: 26097592
    31. The proportion of deaths from extra-colonic cancer was significantly higher in Japanese families with Lynch syndrome with MSH2 mutation PMID: 27069191
    32. Studies indicate that each of the three neoplasm protein variants in BRCA1, BRCA2 and MSH2 would normally be reported as pathogenic based on widely accepted guidelines. PMID: 25639900
    33. foot-drop is frequently associated with NEB gene mutations . This result supports the indication deriving from the yeast model that BRCA1 driven tumorigenesis may be modulated by MSH2. PMID: 26381082
    34. Pathogenic mutations were only confined to MSH2 and identified in 28.8% of Singapore families with Hereditary Colorectal Cancer. PMID: 26477961
    35. s propose an Alu-mediated recombination model to explain the origin of the cryptic paracentric inversion of MSH2 exons 2-6 causes Lynch syndrome. PMID: 26498247
    36. MSH2, MSH6, and EXO1 genes were overexpressed in gastroesophageal cancers. PMID: 26215063
    37. We identified several genes (FasL, MSH2, ABCC5, CASP3, and CYP3A4)that showed association with PFS in patients with osteosarcoma. These pharmacogenetic risk factors might be useful to predict treatment outcome PMID: 25829401
    38. The mismatch-binding protein MutS beta, a heterodimer of MSH2 and MSH3, activates ATR in response to DNA double-strand breaks. PMID: 26212458
    39. Expression levels of DNA repair genes, such as MSH2, may serve as informative biomarkers for identifying patients with T2 Diabetes and coronary artery disease. PMID: 26088318
    40. Prostate cancer occurs at a younger age mainly for mutations in MSH2 gene. PMID: 25640028
    41. Gly322Asp polymorphism of the hMSH2 gene may be linked with Triple-negative breast cancer occurrence in Polish women PMID: 25134804
    42. study identifies an HDAC10-mediated regulatory mechanism controlling the DNA mismatch repair function of MSH2. PMID: 26221039
    43. Results indicate that the expression of hMSH2 is upregulated in ovarian carcinoma cell lines and tissues after paclitaxel treatment, and hMSH2 overexpression is related to paclitaxel resistance and poor prognosis. PMID: 25175513
    44. Somatic rearrangements in MSH2 and MSH6 are an important mechanism leading to hypermutation and microsatellite instability in advanced prostate cancer. PMID: 25255306
    45. A role of MSH2 protein in glioblastoma resistance to temozolomide PMID: 26025730
    46. Report mutations in SNP loci of MSH2 in neoplastic intestinal polyps and colorectal cancer. PMID: 25561800
    47. Intronic SNPs, gene-gene and gene-environment interactions in hMSH2 might be associated with susceptibility to colorectal cancer. PMID: 25560462
    48. A novel mutation consisting in a G deletion at 914 codon of the exon 16 in the MSH2 gene that is associated with HNPCC is described. PMID: 25200962
    49. None of single-nucleotide polymorphisms located at MSH2 were significantly associated with prognosis of colorectal cancer in China. PMID: 24793746
    50. MSH2 founder mutations in Ashkenazi Jews with Lynch syndrome PMID: 23990280

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  • 相关疾病：
    Hereditary non-polyposis colorectal cancer 1 (HNPCC1); Muir-Torre syndrome (MRTES); Endometrial cancer (ENDMC); Mismatch repair cancer syndrome (MMRCS); Colorectal cancer (CRC)
  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    DNA mismatch repair MutS family
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    HGNC: 7325

    OMIM: 114500

    KEGG: hsa:4436

    STRING: 9606.ENSP00000233146

    UniGene: Hs.597656