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MTHFD2 Antibody

  • 货号：
    CSB-PA015156ESR1HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: MTHFD2 antibody at 4.99µg/ml
      Lane 1: 293 whole cell lysate
      Lane 2: A431 whole cell lysate
      Lane 3: HepG2 whole cell lysate
      Lane 4: Hela whole cell lysate
      Lane 5: K562 whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 38, 27 kDa
      Observed band size: 38 kDa
    • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA015156ESR1HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA015156ESR1HU at dilution of 1:100
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) MTHFD2 Polyclonal antibody
  • Uniprot No.：
    P13995
  • 基因名：
    MTHFD2
  • 别名：
    Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase; mitochondrial antibody; Descriptions antibody; Methenyltetrahydrofolate cyclohydrolase antibody; methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 antibody; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) antibody; methylenetetrahydrofolate dehydrogenase 2 antibody; MGC82516 antibody; MTDC_HUMAN antibody; MTHFD2 antibody; mthfd2 methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody; NAD-dependent methylene tetrahydrofolate dehydrogenase antibody; NAD-dependent methylenetetrahydrofolate dehydrogenase antibody; NMDMC antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial protein (40-250AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.
  • 基因功能参考文献：
    1. endothelial cells undergo MTHFD2-mediated reprogramming toward serine-glycine and mitochondrial one-carbon metabolism to compensate for the loss of ATP in response to oxidized phospholipids during atherosclerosis PMID: 29895827
    2. Knocking down MTHFD2 expression in renal cell carcinoma cells, decreased cell proliferation, migration, and invasion were observed and accompanied by the reduced expression of vimentin. PMID: 30466107
    3. miR-92a inhibits proliferation and induces apoptosis by directly regulating MTHFD2 expression in AML. PMID: 28059050
    4. metabolic alterations in MCF7 cells observed as a consequence of MTHFD2 suppression PMID: 27315223
    5. crystal structure of MTHFD2 in complex with a substrate-based inhibitor and the enzyme cofactors NAD(+) and inorganic phosphate. PMID: 27899380
    6. Mechanistically, MYC regulates the expression of MTHFD2, and MTHFD2 knockdown suppresses the TCA cycle. PMID: 27325891
    7. siRNA-mediated silencing of MTHFD2 inhibited migration, invasion and epithelial-mesenchymal transition progression in hepatocellular carcinoma (HCC) cell lines, but no obvious effects on cell proliferation, apoptosis or cell cycle distribution were detected. MTHFD2 is overexpressed in HCC, and is associated with poor prognosis and cellular features connected to metastatic disease. PMID: 27257051
    8. These findings suggest a previously unknown role for MTHFD2 in cancer cell proliferation, adding to its known function in mitochondrial folate metabolism. PMID: 26461067
    9. The highest scoring pathway is mitochondrial one-carbon metabolism and is centred on MTHFD2. PMID: 24451681
    10. Data indicate that methylenetetrahydrofolate dehydrogenase (NADP + -dependent) 2 (MTHFD2)was differentially expressed in breast cancer tissue, suggesting as a prognostic factor and a potential therapeutic target for future breast cancer treatments. PMID: 24870594
    11. Data indicate that the reduced vimentin expression in response to EPHB4, WIPF2 and MTHFD2 silencing was observed at mRNA and protein levels. PMID: 23295955

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  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    Tetrahydrofolate dehydrogenase/cyclohydrolase family
  • 数据库链接：

    HGNC: 7434

    OMIM: 604887

    KEGG: hsa:10797

    STRING: 9606.ENSP00000377617

    UniGene: Hs.469030