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MYRF Antibody

  • 货号：
    CSB-PA897527LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Mouse brain tissue
      All lanes: MYRF antibody at 3.2μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 125, 121 kDa
      Observed band size: 125 kDa
    • Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA897527LA01HU at dilution of 1:100
    • Immunofluorescence staining of A549 cells with CSB-PA897527LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    The peptide mapping within the amino acids 843-1004 of the recombinant human MYRF protein is used to immunize rabbits to get rabbit antiserum. The rabbit antiserum is further purified by protein G to get the anti-MYRF antibody. It is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is greater than 95%. It is reactive with human and mouse MYRF protein, a membrane-bound transcription factor required for oligodendrocyte (OL) differentiation and myelin formation ＆ maintenance. And this MYRF antibody is suitable for the detection of the MYRF protein in ELISA, WB, and IHC application.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) MYRF Polyclonal antibody
  • Uniprot No.：
    Q9Y2G1
  • 基因名：
    MYRF
  • 别名：
    C11orf9 antibody; Chromosome 11 open reading frame 9 antibody; GM1804 antibody; GM98 antibody; KIAA0954 antibody; MGC10781 antibody; MRF antibody; MRF_HUMAN antibody; Myelin gene regulatory factor antibody; Ndt80 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human Myelin regulatory factor protein (843-1004AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，MYRF Antibody (CSB-PA897527LA01HU)，的标记方式是Non-conjugated。对于MYRF Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA897527LB01HU MYRF Antibody, HRP conjugated ELISA
    FITC CSB-PA897527LC01HU MYRF Antibody, FITC conjugated
    Biotin CSB-PA897527LD01HU MYRF Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Constitutes a precursor of the transcription factor. Mediates the autocatalytic cleavage that releases the Myelin regulatory factor, N-terminal component that specifically activates transcription of central nervous system (CNS) myelin genes.; Membrane-bound part that has no transcription factor activity and remains attached to the endoplasmic reticulum membrane following cleavage.; Transcription factor that specifically activates expression of myelin genes such as MBP, MOG, MAG, DUSP15 and PLP1 during oligodendrocyte (OL) maturation, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Specifically recognizes and binds DNA sequence 5'-CTGGYAC-3' in the regulatory regions of myelin-specific genes and directly activates their expression. Not only required during oligodendrocyte differentiation but is also required on an ongoing basis for the maintenance of expression of myelin genes and for the maintenance of a mature, viable oligodendrocyte phenotype.
  • 基因功能参考文献：
    1. The relative scarcity of oligodendrocyte lineage cells expressing MYRF in demyelinated MS lesions demonstrates, for the first time, that chronic lesions lack oligodendrocytes that express this necessary transcription factor for remyelination and supports the notion that a failure to fully differentiate underlies remyelination failure. PMID: 28631093
    2. molecular mechanisms underlying the homo-trimerization of Myrf N-terminal fragments and the homo-trimeric DNA binding of Myrf N-terminal fragments PMID: 28160598
    3. A Bacteriophage tailspike domain promotes self-cleavage of a human membrane-bound transcription factor, the myelin regulatory factor MYRF. PMID: 23966832
  • 亚细胞定位：
    [Myelin regulatory factor]: Endoplasmic reticulum membrane; Single-pass membrane protein.; [Myelin regulatory factor, N-terminal]: Nucleus. Cytoplasm.; [Myelin regulatory factor, C-terminal]: Endoplasmic reticulum membrane; Single-pass membrane protein.
  • 蛋白家族：
    MRF family
  • 组织特异性：
    Expressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina.
  • 数据库链接：

    HGNC: 1181

    OMIM: 608329

    KEGG: hsa:745

    STRING: 9606.ENSP00000278836

    UniGene: Hs.473109