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NEDD1 Antibody

  • 货号：
    CSB-PA822820DSR2HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: NEDD1 antibody at 2.66μg/ml
      Lane 1: Mouse brain tissue
      Lane 2: Jurkat whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 72, 63, 73 kDa
      Observed band size: 72 kDa
    • Immunohistochemistry of paraffin-embedded human glioma using CSB-PA822820DSR2HU at dilution of 1:100
    • Immunoprecipitating NEDD1 in Hela whole cell lysate
      Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA822820DSR2HU in Hela whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
      Lane 2: CSB-PA822820DSR2HU (4μg) + Hela whole cell lysate (500μg)
      Lane 3: Hela whole cell lysate (20μg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) NEDD1 Polyclonal antibody
  • Uniprot No.：
    Q8NHV4
  • 基因名：
    NEDD1
  • 别名：
    FLJ35902 antibody; GCP WD antibody; NEDD-1 antibody; NEDD1 antibody; NEDD1_HUMAN antibody; Neural precursor cell expressed developmentally down-regulated protein 1 antibody; Neural precursor cell expressed, developmentally down regulated gene 1 antibody; Protein NEDD1 antibody; TUBGCP7 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human Protein NEDD1 protein (411-660AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.
  • 基因功能参考文献：
    1. One important function of Aurora A in mitotic cells is to promote microtubule nucleation around the chromatin by phosphorylating NEDD1, and thereby to promote functional spindle assembly. PMID: 23273898
    2. The mice treated with small interfering (si) RNA targeting NEDD1 by the atelocollagen-mediated delivery system showed a significantly prolonged survival in a xenograft model of human gastric cancer. PMID: 23106787
    3. Data suggest that the NEDD1/gamma-tubulin interaction is finely tuned by multiple phosphorylation events in the S557-S574 region and is critical for spindle assembly. PMID: 22595525
    4. a direct interaction with NEDD1 regulates gamma-tubulin recruitment to the centrosome PMID: 20224777
    5. GCP-WD (NEDD1) broadly mediates targeting of the gamma-tubulin ring complex to sites of microtubule nucleation and to the mitotic spindle, which is essential for spindle formation. PMID: 16378099
    6. The NEED1 protein was characterized and its function in centriole duplication and spindle assembly determined. PMID: 16461362
    7. Data propose NEDD1 may be a promising target for controlling cell proliferation, in particular if targeted in combination with Plk1 inhibitors. PMID: 19243593
    8. Data suggest that sequential phosphorylation of Nedd1 by Cdk1 and Plk1 plays a role in targeting gamma tubulin ring complex to the centrosome by promoting the interaction of Nedd1 with the gammaTuRC component gamma-tubulin, during mitosis. PMID: 19509060

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 数据库链接：

    HGNC: 7723

    OMIM: 600372

    KEGG: hsa:121441

    STRING: 9606.ENSP00000451211

    UniGene: Hs.270084