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NFE2L1 Antibody

  • 货号：
    CSB-PA690633
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA690633(NFE2L1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA690633(NFE2L1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
    • Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Raji cells, hepG2 cells, hela cells, Primary antibody: CSB-PA690633(NFE2L1 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
  • 其他：

产品详情

  • Uniprot No.：
    Q14494
  • 基因名：
    NFE2L1
  • 别名：
    erythroid derived 2 antibody; like 1 antibody; Locus control region factor 1 antibody; Locus control region-factor 1 antibody; NF-E2-related factor 1 antibody; NF2L1_HUMAN antibody; NFE2 related factor 1 antibody; NFE2-related factor 1 antibody; NFE2L1 antibody; NRF1 antibody; Nuclear factor antibody; Nuclear Factor Erythroid 2 Related Factor 1 antibody; Nuclear factor erythroid 2-related factor 1 antibody; Nuclear factor erythroid derived 2 like 1 antibody; TCF-11 antibody; TCF11 antibody; Transcription factor 11 antibody; Transcription factor HBZ17 antibody; Transcription factor LCR F1 antibody; Transcription factor LCR-F1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Fusion protein of Human NFE2L1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    WB 1:200-1:1000
    IHC 1:25-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Endoplasmic reticulum membrane sensor that translocates into the nucleus in response to various stresses to act as a transcription factor. Constitutes a precursor of the transcription factor NRF1. Able to detect various cellular stresses, such as cholesterol excess, oxidative stress or proteasome inhibition. In response to stress, it is released from the endoplasmic reticulum membrane following cleavage by the protease DDI2 and translocates into the nucleus to form the transcription factor NRF1. Acts as a key sensor of cholesterol excess: in excess cholesterol conditions, the endoplasmic reticulum membrane form of the protein directly binds cholesterol via its CRAC motif, preventing cleavage and release of the transcription factor NRF1, thereby allowing expression of genes promoting cholesterol removal, such as CD36. Involved in proteasome homeostasis: in response to proteasome inhibition, it is released from the endoplasmic reticulum membrane, translocates to the nucleus and activates expression of genes encoding proteasome subunits.; CNC-type bZIP family transcription factor that translocates to the nucleus and regulates expression of target genes in response to various stresses. Heterodimerizes with small-Maf proteins (MAFF, MAFG or MAFK) and binds DNA motifs including the antioxidant response elements (AREs), which regulate expression of genes involved in oxidative stress response. Activates or represses expression of target genes, depending on the context. Plays a key role in cholesterol homeostasis by acting as a sensor of cholesterol excess: in low cholesterol conditions, translocates into the nucleus and represses expression of genes involved in defense against cholesterol excess, such as CD36. In excess cholesterol conditions, the endoplasmic reticulum membrane form of the protein directly binds cholesterol via its CRAC motif, preventing cleavage and release of the transcription factor NRF1, thereby allowing expression of genes promoting cholesterol removal. Critical for redox balance in response to oxidative stress: acts by binding the AREs motifs on promoters and mediating activation of oxidative stress response genes, such as GCLC, GCLM, GSS, MT1 and MT2. Plays an essential role during fetal liver hematopoiesis: probably has a protective function against oxidative stress and is involved in lipid homeostasis in the liver. Involved in proteasome homeostasis: in response to proteasome inhibition, mediates the 'bounce-back' of proteasome subunits by translocating into the nucleus and activating expression of genes encoding proteasome subunits. Also involved in regulating glucose flux. Together with CEBPB; represses expression of DSPP during odontoblast differentiation. In response to ascorbic acid induction, activates expression of SP7/Osterix in osteoblasts.
  • 基因功能参考文献：
    1. Data show that Nrf1 can adopt several topologies within membranes that are determined by its NTD. PMID: 20629635
    2. Fbw7 as a regulator of Nrf1 expression and reveal a novel function of Fbw7 in cellular stress response. PMID: 21953459
    3. results indicate a novel role of Nrf1 in UVB-induced DNA damage repair and suggest Nrf1 as a tumor suppressor in the skin PMID: 22500024
    4. Data indicate both Nrf1a and Nrf1b isoforms transcripts were detected in different mouse and human cell lines, and in various mouse tissues. PMID: 23144760
    5. Nrf1 knockout resulted in the increased expression of GCLC and GCLM in human bronchial epithelial (HBE1) cells. PMID: 24024152
    6. while Nrf2 is crucial for MRE/ARE-mediated transcription in response to copper, Nrf1 may activate MT-1 expression by a mechanism different from that Nrf2 employs. PMID: 24462598
    7. Nrf1 physically interacts with androgen receptor and enhances it's DNA-binding activity, suggesting that the p65-Nrf1 isoform is a potential androgen receptor coactivator. PMID: 24466341
    8. The treatment with E2, Res, or VC significantly increased mRNA and protein expression levels of Nrf1. PMID: 25130429
    9. The results indicate that Nrf1 is a transcriptional activator of Herpud1 expression during ER stress, and they suggest Nrf1 is a key player in the regulation of the ER stress response in cells. PMID: 25637874
    10. This review outlines Nrf1 structure, function, regulation and its links to insulin resistance, diabetes and inflammation. PMID: 26254094
    11. Importantly, Nrf1 activity is positively and/or negatively regulated by distinct doses of proteasome and calpain inhibitors PMID: 26268886
    12. Together with molecular expression results, the s thus suppose requirement of Nrf1alpha (and major derivates) for gene regulatory mechanisms repressing cancer cell process and malignant behaviour. PMID: 27065079
    13. These results uncover a new regulatory mechanism that USP15 activates Nrf1 against the beta-TrCP inhibition to maintain proteostasis. PMID: 27416755
    14. Here the s show that the aspartyl protease DNA-damage inducible 1 homolog 2 (DDI2) is required to cleave and activate Nrf1. PMID: 27528193
    15. Nrf1 is regulated by O-GlcNAc transferase. PMID: 28625484
    16. our investigation shows that the ER membrane protein TCF11/Nrf1 is an essential component of the cellular stress response mechanism. In response to cytotoxic stress, TCF11/Nrf1 is retrotranslocated and transferred to the nucleus where it induces proteasome subunit expression via binding to the ARE region of the relevant promoter. PMID: 27345029
    17. Data uncover an unexpected role for p97 in activation of transcription factor Nrf1 by relocalizing it from the endoplasmic reticulum lumen to the cytosol. PMID: 24448410
    18. Nrf1 is negatively regulated by its O-GlcNAcylation status that depends on the glucose concentrations PMID: 26231763
    19. A proteasome inhibitor-stimulated Nrf1 protein-dependent compensatory increase in proteasome subunit gene expression reduces polycomb group protein level PMID: 22932898
    20. The study identifies casein kinase 2 as an NRF1-binding protein and finds that the knockdown of casein kinase 2 enhances the NRF1-dependent expression of the proteasome subunit genes. PMID: 23816881
    21. GSK3 regulates Nrf1 expression and cell survival function in response to stress activation. PMID: 23623971
    22. findings suggest that nuclear-related factor erythroid 2 transcription factor (Nrf1) may play a role in maintaining genomic integrity, and that Nrf1 dysregulation may induce tumorigenesis PMID: 22971132
    23. data suggests that Nrf1 is controlled by several post-translational mechanisms, including ubiquitination, proteolytic processing and proteasomal-mediated degradation as well as by its phosphorylation status PMID: 22216197
    24. Report that proteasome inhibition can be anticipated by TCF11-dependent activation of human proteasome gene expression, which in turn is controlled by a HRD1-p97-ERAD feedback circle. PMID: 20932482
    25. Data show that nuclear factor erythroid-derived 2-related factor 1 (Nrf1), but not the related Nrf2, is necessary for induced proteasome gene transcription in mouse embryonic fibroblasts. PMID: 20385086
    26. A nuclear export signal (NES) localised in the N-terminus of TCF11 is responsible for the active nuclear export of the protein. PMID: 12729924
    27. p65Nrf1 has the potential to play an important role in modulating the response to oxidative stress by functioning as a transdominant repressor of Nrf2-mediated activation of ARE-dependent gene transcription PMID: 17609210

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  • 亚细胞定位：
    [Endoplasmic reticulum membrane sensor NFE2L1]: Endoplasmic reticulum membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein.; [Transcription factor NRF1]: Nucleus.
  • 蛋白家族：
    BZIP family, CNC subfamily
  • 数据库链接：

    HGNC: 7781

    OMIM: 163260

    KEGG: hsa:4779

    STRING: 9606.ENSP00000354855

    UniGene: Hs.514284