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NFS1 Antibody

  • 货号：
    CSB-PA896542ESR1HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: NFS1 antibody at 6.21µg/ml
      Lane 1: HepG2 whole cell lysate
      Lane 2: Hela whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 51, 45 kDa
      Observed band size: 51 kDa
    • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA896542ESR1HU at dilution of 1:100
    • Immunoprecipitating NFS1 in Hela whole cell lysate
      Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA896542ESR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
      Lane 2: CSB-PA896542ESR1HU (4µg) + Hela whole cell lysate (500µg)
      Lane 3: Hela whole cell lysate (20µg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) NFS1 Polyclonal antibody
  • Uniprot No.：
    Q9Y697
  • 基因名：
    NFS1
  • 别名：
    Cysteine desulfurase antibody; Cysteine desulfurase; mitochondrial antibody; HUSSY 08 antibody; IscS antibody; mitochondrial antibody; NFS1 antibody; NFS1 nitrogen fixation 1 antibody; NFS1 nitrogen fixation 1 homolog (S. cerevisiae) antibody; NFS1_HUMAN antibody; NIFS antibody; nitrogen fixation 1 (S. cerevisiae; homolog) antibody; nitrogen fixation gene 1 antibody; nitrogen-fixing bacteria S; homolog of antibody; nitrogen-fixing bacteria S-like protein antibody; OTTHUMP00000030799 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Cysteine desulfurase, mitochondrial protein (208-457AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor.
  • 基因功能参考文献：
    1. FXN binds the NFS1-ISD11-ACP-ISCU complex (SDAU), to activate the desulfurase activity and Fe-S cluster biosynthesis. In the absence of FXN, the NFS1-ISD11-ACP (SDA) complex was reportedly inhibited by binding of recombinant ISCU PMID: 30031876
    2. analysis of the NFS1-ISD11-ACP (SDA) complex forms the core of the iron-sulfur (Fe-S) assembly complex and associates with assembly proteins ISCU2, frataxin (FXN), and ferredoxin to synthesize Fe-S clusters PMID: 28634302
    3. NFS1 maintains the iron sulfur clusters in proteins that are essential for protecting them from oxidative damage; inactivating NFS1 or iron sulfur clusters can trigger ferroptosis, a non-apoptotic form of cell death, in cancer cells PMID: 29168506
    4. human NFS1 was almost fully able to complement the role of IscS in Moco biosynthesis when its specific interaction partner protein MOCS3 from humans was also present. PMID: 28766335
    5. The NFS1/ISD11 complex further interacts with scaffold protein ISCU and regulator protein frataxin, thereby forming a quaternary complex for Fe-S cluster formation. PMID: 28271877
    6. FDX1 and FDX2 both bind NFS1 and donate electrons for iron-sulfur cluster biosynthesis. PMID: 28001042
    7. Molecular dynamics flexible fitting of protein structures docked into the EM map of the model revealed a [FXN(42-210)]24.[NFS1]24.[ISD11]24.[ISCU]24 complex, consistent with the measured 1:1:1:1 stoichiometry of its four components. PMID: 27519411
    8. Our findings highlight that the ISD11 R68A/R68L mutation display reduced affinity to form a stable subcomplex with NFS1, and thereby fails to prevent NFS1 aggregation resulting in impairment of the Fe-S cluster biogenesis PMID: 26342079
    9. The data presented here show that the Isu1 suppressor mimics the frataxin effects on Nfs1, explaining the bypassing activity. PMID: 24217246
    10. NFS1 binds preferentially to the D-state of ISCU while mtHSP70 binds preferentially to the D-state of ISCU and HSC20 binds preferentially to the S-state of ISCU. PMID: 23940031
    11. the interaction of NFS1 and MOCS3 in the cytosol of human cells, is reported. PMID: 23593335
    12. Nfs1, the cysteine desulfurase responsible for providing sulfur for cluster formation, is required for the increased Isu stability occurring after disruption of cluster formation on or transfer from Isu PMID: 22689995
    13. the cytosolic form of ISCS is a functional cysteine desulfurase that can collaborate with cytosolic ISCU to promote de novo iron-sulfur cluster formation PMID: 16527810
    14. Results show that human Nfs1 is required inside mitochondria for efficient maturation of cellular iron/sulfur proteins. PMID: 16847322
    15. Nfs1 acts as a sulfur donor for MOCS3, a protein involved in molybdenum cofactor biosynthesis PMID: 18650437
    16. Icp55 protease and its substrate Nfs1 appear to be dual distributed between the nucleus and mitochondria PMID: 19720832

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  • 亚细胞定位：
    [Isoform Mitochondrial]: Mitochondrion.; [Isoform Cytoplasmic]: Cytoplasm. Nucleus.
  • 蛋白家族：
    Class-V pyridoxal-phosphate-dependent aminotransferase family, NifS/IscS subfamily
  • 组织特异性：
    Predominantly expressed in heart and skeletal muscle. Also found in brain, liver and pancreas.
  • 数据库链接：

    HGNC: 15910

    OMIM: 603485

    KEGG: hsa:9054

    STRING: 9606.ENSP00000363205

    UniGene: Hs.194692