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  4. NR1D1 Antibody

NR1D1 Antibody

  • 货号：
    CSB-PA099477
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using CSB-PA099477(NR1D1 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA099477(NR1D1 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    P20393
  • 基因名：
    NR1D1
  • 别名：
    EAR-1 antibody; EAR1 antibody; ERBA-related 1 antibody; hRev antibody; Nr1d1 antibody; NR1D1_HUMAN antibody; Nuclear receptor Rev ErbA alpha antibody; Nuclear receptor subfamily 1 group D member 1 antibody; Rev erbAalpha antibody; Rev erbalpha antibody; Rev-erbA-alpha antibody; Rev-ErbAalpha antibody; Reverba antibody; THRA1 antibody; THRAL antibody; Thyroid hormone receptor; alpha like antibody; Thyroid hormone receptor; alpha-1- like antibody; V-erbA-related protein 1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Fusion protein of Human NR1D1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components ARTNL/BMAL1, CLOCK and CRY1. Also regulates genes involved in metabolic functions, including lipid and bile acid metabolism, adipogenesis, gluconeogenesis and the macrophage inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5'-[A/G]GGTCA-3' preceded by an A/T-rich 5' sequence (RevRE), or as a homodimer to a direct repeat of the core motif spaced by two nucleotides (RevDR-2). Acts as a potent competitive repressor of ROR alpha (RORA) function and regulates the levels of its ligand heme by repressing the expression of PPARGC1A, a potent inducer of heme synthesis. Regulates lipid metabolism by repressing the expression of APOC3 and by influencing the activity of sterol response element binding proteins (SREBPs); represses INSIG2 which interferes with the proteolytic activation of SREBPs which in turn govern the rhythmic expression of enzymes with key functions in sterol and fatty acid synthesis. Regulates gluconeogenesis via repression of G6PC1 and PEPCK and adipocyte differentiation via repression of PPARG. Regulates glucagon release in pancreatic alpha-cells via the AMPK-NAMPT-SIRT1 pathway and the proliferation, glucose-induced insulin secretion and expression of key lipogenic genes in pancreatic-beta cells. Positively regulates bile acid synthesis by increasing hepatic expression of CYP7A1 via repression of NR0B2 and NFIL3 which are negative regulators of CYP7A1. Modulates skeletal muscle oxidative capacity by regulating mitochondrial biogenesis and autophagy; controls mitochondrial biogenesis and respiration by interfering with the STK11-PRKAA1/2-SIRT1-PPARGC1A signaling pathway. Represses the expression of SERPINE1/PAI1, an important modulator of cardiovascular disease and the expression of inflammatory cytokines and chemokines in macrophages. Represses gene expression at a distance in macrophages by inhibiting the transcription of enhancer-derived RNAs (eRNAs). Plays a role in the circadian regulation of body temperature and negatively regulates thermogenic transcriptional programs in brown adipose tissue (BAT); imposes a circadian oscillation in BAT activity, increasing body temperature when awake and depressing thermogenesis during sleep. In concert with NR2E3, regulates transcriptional networks critical for photoreceptor development and function. In addition to its activity as a repressor, can also act as a transcriptional activator. In the ovarian granulosa cells acts as a transcriptional activator of STAR which plays a role in steroid biosynthesis. In collaboration with SP1, activates GJA1 transcription in a heme-independent manner. Represses the transcription of CYP2B10, CYP4A10 and CYP4A14. Represses the transcription of CES2. Represses and regulates the circadian expression of TSHB in a NCOR1-dependent manner. Negatively regulates the protein stability of NR3C1 and influences the time-dependent subcellular distribution of NR3C1, thereby affecting its transcriptional regulatory activity. Plays a critical role in the circadian control of neutrophilic inflammation in the lung; under resting, non-stress conditions, acts as a rhythmic repressor to limit inflammatory activity whereas in the presence of inflammatory triggers undergoes ubiquitin-mediated degradation thereby relieving inhibition of the inflammatory response. Plays a key role in the circadian regulation of microglial activation and neuroinflammation; suppresses microglial activation through the NF-kappaB pathway in the central nervous system. Plays a role in the regulation of the diurnal rhythms of lipid and protein metabolism in the skeletal muscle via transcriptional repression of genes controlling lipid and amino acid metabolism in the muscle.
  • 基因功能参考文献：
    1. Data suggest that MYC induction of REV-ERBalpha is both persistent and recurrent across many inducible MYC model systems. PMID: 28332504
    2. NR1D1 interacted with poly(ADP-ribose) polymerase 1 (PARP1) and subsequently inhibited catalytic activity of PARP1. PMID: 28599788
    3. NR1D1 and BMAL1 mRNA and protein levels were significantly reduced in OA compared to normal cartilage. In cultured human chondrocytes, a clear circadian rhythmicity was observed for NR1D1 and BMAL1. PMID: 27884645
    4. To determine the impact of REV-ERBalpha activation in the cigarette smoke (CS)-induced lung inflammatory response, we treated primary small airway epithelial cells with CS extract or lipopolysaccharide in the absence or presence of pre-treatment with the REV-ERBalpha agonist GSK 4112. PMID: 28974420
    5. Downregulation of NR1D1 in MCF7 cells resulted in resistance to doxorubicin, both in vitro and in vivo Analysis of four public patient data sets indicated that NR1D1 expression correlates positively with clinical outcome in breast cancer patients who received chemotherapy. Our findings suggest that NR1D1 and its ligands provide therapeutic options that could enhance the outcomes of chemotherapy in breast cancer patients PMID: 28249904
    6. Highly quantitative fluorescence anisotropy assays in competition mode revealed that the rev-erbA-alpha specificity for the NCoR corepressor lies in the first two residues of the beta-strand in Interaction Domain 1 of NCoR. PMID: 25969949
    7. Data show that ubiquitin E3 ligase Siah2 depletion delays circadian degradation of nuclear hormone receptor RevErbalpha (Nr1d1) and lengthens period length. PMID: 26392558
    8. the role of NR1D1 polymorphisms in the regulation of Nuclear receptor REV-ERBalpha and circadian rhythms regulation PMID: 25798852
    9. associations between NR1D1, RORA and RORB genes and bipolar disorder.( PMID: 25789810
    10. CRY2 and REV-ERB ALPHA as the clock genes upregulated in obesity during the 24 h period and that REV-ERB ALPHA is an important gene associated with MS. PMID: 25365257
    11. Our results suggest that the REV-ERB ALPHA rs939347 polymorphism could modulate body fat mass in men. The present work supports the role of REV-ERB ALPHA in the development of obesity as well as a potential target for the treatment of obesity PMID: 25089907
    12. Using free-energy simulations, the study shows that rev-erbA-alpha-induced DNA deformation preferentially occurs by induced fit rather than by conformational selection, even though the DNA is only slightly distorted in the complex. PMID: 24677802
    13. Rev-erbalpha is a novel regulator of hepatic stellate cell transdifferentation PMID: 24497272
    14. apoA-IV inhibits hepatic gluconeogenesis by decreasing Glc-6-Pase and PEPCK gene expression through NR1D1. PMID: 24311788
    15. Rev-erbalpha bestows protection against mycobacterial infection by direct gene repression of IL10 and thus provide a novel target in modulating macrophage microbicidal properties. PMID: 23449984
    16. DBC1 modulates the stability and function of the nuclear receptor Rev-erb-alpha. PMID: 23398316
    17. A haplotype block in REV-ERBalpha was associated with white matter lesion volumes in the Rotterdam Study I. PMID: 23083441
    18. Genetic variants of NR1D1 associate with bipolar disorder. PMID: 22538398
    19. Thyroid hormone receptor-alpha/NR1D1 polymorphisms were not associated with baseline characteristics, including serum TSH and free thyroxine. None of the polymorphisms were associated with bone mineral density or osteoporotic fractures. PMID: 22224817
    20. in situ hybridization revealed that RORA and NR1D1 were expressed in human endometrial stromal and epithelial cells PMID: 22024429
    21. REV-ERBalpha plays a dual role in regulation of the activity of the BMAL1/CLOCK heterodimer by regulation of expression of both the BMAL1 and CLOCK genes. PMID: 21479263
    22. Data demonstrate that NPAS2 is also a RORalpha and REV-ERBalpha target gene. PMID: 20817722
    23. Study reports the crystal structure of a nuclear receptor-co-repressor (N-CoR) interaction domain 1 (ID1) peptide bound to truncated human Rev-erbalpha ligand-binding domain. PMID: 20581824
    24. Arf-bp1 and Pam are novel regulators of circadian gene expression that target Rev-erb alpha for degradation PMID: 20534529
    25. Results show that the cells of this aggressive form of breast cancer are genetically preprogrammed to depend on NR1D1 and PBP for the energy production necessary for survival. PMID: 20160030
    26. Review. We propose that the nuclear receptor and core clock component Rev-erb-alpha behaves as a gatekeeper to timely coordinate the circadian metabolic response. PMID: 19696364
    27. While negative, our findings do not exclude an involvement of DGKH and NR1D1 in lithium prophylaxis. PMID: 19818381
    28. novel physiological role for members of the Rev-erb family of nuclear receptors in the regulation of genes involved in triglyceride metabolism and the pathogenesis of atherosclerosis PMID: 12021280
    29. ROR(alpha) and Reverb(alpha) are expressed with a similar tissue distribution and are both induced during the differentiation of rat L6 myoblastic cells PMID: 12114512
    30. hRev-erbalpha plays a key role in hRORalpha1 action PMID: 12377782
    31. Data show that Rev-erb alpha and beta contain a functional nuclear location signal in the DNA-binding domain, and suggest that they control their intracellular localization via a mechanism different from that of other nuclear receptors. PMID: 12683943
    32. Rev-Erbalpha as a target gene of PPARgamma in adipose tissue and demonstrate a role for this nuclear receptor as a promoter of adipocyte differentiation. PMID: 12821652
    33. Overexpression of EAR1 upregulated expression of IL6 and COX2, and increased transactivation by NFKB and nuclear translocation of p65 in A7r5 VSMCs. The expression of EAR1 was upregulated by RORalpha1 but that upregulation was attenuated by EAR1 itself PMID: 15013753
    34. findings show that GSK3beta phosphorylates and stabilizes Rev-erbalpha, a negative component of the circadian clock; control of Rev-erbalpha protein stability is a critical component of the peripheral clock and a biological target of lithium therapy PMID: 16484495
    35. Rev-ErbAalpha is highly expressed in osteoarthritis articular chondrocytes and that its expression is modulated by known cartilage catabolic and anabolic stimuli PMID: 17075855
    36. findings show that rev-erbalpha serves as a heme sensor that coordinates the cellular clock, glucose homeostasis, and energy metabolism PMID: 18006707
    37. heme regulation of REV-ERBs may link the control of metabolism and the mammalian clock PMID: 18037887
    38. Rev-erbalpha protein levels must rise and then fall for adipocyte differentiation to occur. Stable expression of Rev-erb protein prevents induction of PPARgamma2. PMID: 18227153
    39. These data identify Rev-erbalpha as a new LXR target gene, inhibiting LXR-induction of TLR-4 in a negative transcriptional feedback loop, but not cholesterol homeostasis gene expression. PMID: 18511497
    40. This study suggests that NR1D1 does not play a major role in the pathophysiology of mood disorders in the Japanese population. PMID: 18804497
    41. This study indicated a nominal association of the REV-ERBalpha gene with bipolar disorder, suggest a possible role of REV-ERBalpha in the pathogenesis of bipolar disorder. PMID: 19267705
    42. Rev-erbalpha modulates the synthesis of its own ligand in a negative feedback pathway that maintains heme levels and regulates cellular energy metabolism. PMID: 19710360

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Cell projection, dendrite. Cell projection, dendritic spine.
  • 蛋白家族：
    Nuclear hormone receptor family, NR1 subfamily
  • 组织特异性：
    Widely expressed. Expressed at high levels in the liver, adipose tissue, skeletal muscle and brain. Also expressed in endothelial cells (ECs), vascular smooth muscle cells (VSMCs) and macrophages. Expression oscillates diurnally in the suprachiasmatic nuc
  • 数据库链接：

    HGNC: 7962

    OMIM: 602408

    KEGG: hsa:9572

    STRING: 9606.ENSP00000246672

    UniGene: Hs.592130