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PAF1 Antibody

  • 货号：
    CSB-PA017382GA01HU
  • 规格：
    ￥3,900
  • 其他：

产品详情

  • Uniprot No.：
    Q8N7H5
  • 基因名：
    PAF1
  • 别名：
    F23149 1 antibody; FLJ11123 antibody; hPAF1 antibody; PAF 1 antibody; PAF1 antibody; Paf1 RNA polymerase II associated factor homolog antibody; PAF1_HUMAN antibody; Pancreatic differentiation protein 2 antibody; PD 2 antibody; PD2 antibody; PD2 protein antibody; RNA polymerase II associated factor homolog antibody; RNA polymerase II-associated factor 1 homolog antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Human PAF1
  • 免疫原种属：
    Homo sapiens (Human)
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro.
  • 基因功能参考文献：
    1. a subset of enhancers can primarily modulate gene expression by controlling the release of paused Pol II in a PAF1-dependent manner. PMID: 28860207
    2. These results reveal ubiquitin-proteasome system regulation of Paf1 and suggest downregulation of ubiquitin-proteasome system in elevating Paf1's abundance in poorly differentiated cancers. PMID: 29023102
    3. The various functions of Paf1C, such as the regulation of promoter-proximal pausing and development in higher eukaryotes, are complex and context dependent. PMID: 28870425
    4. High PAF1 expression is associated with ovarian cancer. PMID: 28122356
    5. Overexpression of PD2 leads to increased tumorigenicity and metastasis in pancreatic ductal adenocarcinoma. PMID: 26689992
    6. Propose that degradation of MYC limits the accumulation of MYC/PAF1C complexes during transcriptional activation. PMID: 26687678
    7. Mechanistic studies indicated that PAF1C could promote lung cancer cell proliferation through regulating c-MYC transcription. PMID: 26282204
    8. CNOT4 controls the degradation of chromatin-unbound PAF1 via the 26S proteasome. PMID: 25933433
    9. this study found that Pol II-associated factor 1 (PAF1) is a critical regulator of paused Pol II release, that positive transcription elongation factor b (P-TEFb) directly regulates the initial recruitment of PAF1 complex (PAF1C) to genes, and that the subsequent recruitment of CDK12 is dependent on PAF1C. PMID: 26659056
    10. This study reveals an evolutionarily conserved role for PAF1 as a regulator of promoter-proximal pausing by RNA Pol II in all metazoans, including human. PMID: 26279188
    11. PD2 is a novel cancer stem cell (CSC) maintenance protein, loss of which renders the CSCs more susceptible to drug-induced cell death. PMID: 25003666
    12. Together, these results indicate that human adenovirus E1A uses hBre1 to recruit the hPaf1 complex in order to optimally activate viral early transcription by enhancing transcriptional elongation. PMID: 24600005
    13. The results show that the Paf1/Leo1 heterodimer is necessary for its binding to histone H3, the histone octamer, and nucleosome in vitro. PMID: 24038468
    14. E1A changes the function of hBre1 from a ubiquitin ligase involved in substrate selection to a scaffold which recruits hPaf1 as a means to stimulate transcription and transcription-coupled histone modifications. PMID: 23785282
    15. Data indicate that the Plus3 domain of the Rtf1 subunit mediates Paf1C recruitment to genes by binding a repeating domain within the phosphorylated elongation factor Spt5. PMID: 24101474
    16. Data show that activation-induced deaminase (AID) associates with RNA polymerase-associated factor 1 (PAF1) through its N-terminal domain. PMID: 23008333
    17. PAF1c acts as an arginine methyl histone effector that is recruited to promoters and activates a subset of genes, including targets of estrogen signaling. PMID: 22451921
    18. that hPaf1/PD2 in association with MLL1 regulates methylation of H3K4 residues, as well as interacts and regulates nuclear shuttling of chromatin remodeling protein CHD1, facilitating its function in pancreatic cancer cells PMID: 22046413
    19. Interaction between the Polymerase Associated Factor complex and Mixed Lineage Leukemia is critical for gene rearranged leukemogenesis. PMID: 21037944
    20. MLL interacts directly with the polymerase associated factor complex (PAFc) via Paf1 and CTR9. PAFc augments MLL and MLL-AF9 mediated transcriptional activation of Hoxa9 and their interaction is essential for leukemogenesis. PMID: 20541477
    21. Study demonstrates the ability of human PAF1C to facilitate activator p53- and histone acetyltransferase p300-dependent transcription elongation from a recombinant chromatin template in a biochemically defined RNA polymerase II transcription system. PMID: 20178742
    22. hPaf1 and the hPAF complex are key regulators of cell-cycle progression PMID: 19771162
    23. Formation of trimeric complex UbcH6 and RNF20/40 with PAF stimulates histone 2B monoubiquitination activity in vitro PMID: 16307923
    24. Overexpression of PD2/hPaf1 is involved in cancer development. PMID: 16491129

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  • 亚细胞定位：
    Nucleus. Note=Punctuate distribution throughout the nucleus except in nucleoli and the perinuclear chromatin.
  • 蛋白家族：
    PAF1 family
  • 数据库链接：

    HGNC: 25459

    OMIM: 610506

    KEGG: hsa:54623

    STRING: 9606.ENSP00000221265

    UniGene: Hs.466714