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PARD3 Antibody

  • 货号：
    CSB-PA819481LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA819481LA01HU at dilution of 1:100
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, A375 whole cell lysate, K562 whole cell lysate
      All lanes: PARD3 antibody at 3µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 152, 142, 147, 139, 114, 109, 110, 150 kDa
      Observed band size: 152 kDa
    • IHC image of CSB-PA819481LA01HU diluted at 1:600 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of A549 cells with CSB-PA819481LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PARD3 Polyclonal antibody
  • Uniprot No.：
    Q8TEW0
  • 基因名：
  • 别名：
    agouti signaling protein antibody; ASIP antibody; Atypical PKC isotype specific interacting protein antibody; Atypical PKC isotype-specific-interacting protein antibody; Baz antibody; bazooka antibody; Coagulation factor II receptor-like 2 antibody; CTCL tumor antigen se2-5 antibody; F2rl2 antibody; PAR-3 antibody; par-3 family cell polarity regulator alpha antibody; par-3 family cell polarity regulator antibody; PAR3 alpha antibody; par3 antibody; PAR3-alpha antibody; PARD-3 antibody; Pard3 antibody; PARD3_HUMAN antibody; PARD3A antibody; Partitioning defective 3 homolog antibody; Partitioning-defective 3 homolog antibody; Partitioning-defective protein 3; C. elegans; homolog of antibody; SE2-5L16 antibody; SE2-5LT1 antibody; SE2-5T2 antibody; Thrombin receptor-like 2 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Partitioning defective 3 homolog protein (1068-1356AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，PARD3 Antibody (CSB-PA819481LA01HU)，的标记方式是Non-conjugated。对于PARD3 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA819481LB01HU PARD3 Antibody, HRP conjugated ELISA
    FITC CSB-PA819481LC01HU PARD3 Antibody, FITC conjugated
    Biotin CSB-PA819481LD01HU PARD3 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:100-1:1000
    IF 1:50-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Involved in Schwann cell peripheral myelination. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
  • 基因功能参考文献：
    1. Studies indicate that the PAR3-PAR6-aPKC complex are important for the establishment of neuronal polarity [Review]. PMID: 29696344
    2. that this heterozygotic microdeletion showed no tissue specificity and led to defective expression of PARD3 PMID: 28623428
    3. The s identified a tumor-suppressive property function of Par3 in human cervical cancer and the lost Par3 expression may be a marker of poor prognosis. PMID: 29953780
    4. We first demonstrate that Hook2 is essential for the polarized Golgi re-orientation towards the migration front. Depletion of Hook2 results in a decrease of PAR6alpha at the centrosome during cell migration, while overexpression of Hook2 in cells induced the formation of aggresomes with the recruitment of PAR6alpha, aPKC and PAR3 PMID: 27624926
    5. elevated expression of Par3 promotes PCa metastasis via KIBRA sequestration-mediated inactivation of the Hippo pathway to upregulate expression of pro-metastatic genes. PMID: 29017577
    6. reduced expression of PKCzeta/Pard3/Pard6 contributes to non-small-cell lung cancer epithelial-mesenchymal transition, invasion, and chemoresistance. PMID: 28652146
    7. PARD3 might play a tumor suppressor role in esophageal squamous cell carcinoma. PMID: 28526815
    8. Loss of Par3 promotes metastatic behavior in lung adenocarcinoma cells through 14-3-3zeta protein. PMID: 27588399
    9. Studies suggest that rare deleterious variants of PARD3 in the aPKC-binding region contribute to human cranial neural tube defect (NTD). PMID: 27925688
    10. These data highlight the importance of the carboxy-terminal motif of the E6 protein and downregulation of PAR3 in tumorigenic transformation of human cervical keratinocytes. PMID: 28440909
    11. Results suggest that Par3 expression is likely involved in ovarian cancer progression, especially in peritoneal metastasis, probably through modulating IL-6 /STAT3 signaling. PMID: 27855669
    12. reduced keratinocytes Par3 function fosters a permissive P-cadherin-dependent niche for melanocytes transformation, invasion, and metastasis. PMID: 28096290
    13. These results demonstrate the importance of Par3 and SNAIL in development of KSHV-induced B-cells cancers PMID: 27463802
    14. PAR-3 protein expression is frequently lost in primary esophageal squamous cell carcinoma and loss of the PAR-3 protein is associated with aggressive clinicopathological features. PMID: 28188749
    15. Taken together, these results suggest that the Par3 regulates invasion and metastasis in pancreatic cancers by controlling tight junction assembly via Tiam1. PMID: 26084985
    16. Knockdown of the polarity protein Par3 reversed the effects of Galpha13 knockdown on cell-cell adhesion and proteolytic invasion in three-dimensional collagen. PMID: 26589794
    17. Par-3 plays an important role in the modulation of intestinal barrier function by regulating delivery of occludin as well as suppression of MLC phosphorylation. PMID: 25820151
    18. Shp2 promotes metastasis of prostate cancer by attenuating the PAR3/PAR6/aPKC polarity protein complex and enhancing epithelial-to-mesenchymal transition PMID: 26050620
    19. study indicated a potential molecular basis for cell growth-promoting function of PARD3 by modulating the Hippo pathway signaling in response to cell contact and cell polarity signals PMID: 26116754
    20. PAR3-mutant proteins exhibit a relative reduction in the ability to mediate formation of tight junctions and actin-based protrusions and activate STAT3 at cell confluence. PMID: 25833829
    21. PAR3 and aPKC control the organization of the Golgi through CLASP2 phosphorylation. PMID: 25518939
    22. Data show that increased partitioning defective 3 (Par-3) expression is associated with distant metastasis and poor survival rates in patients with hepatocellular carcinoma (HCC). PMID: 23322019
    23. A cytoplasmic expression of PAR-3 is therefore implicated in worse clinical and pathological cancer features in clear cell renal cell carcinoma and could be useful to identify patients with high-risk tumors. PMID: 24856572
    24. The PAR polarity complex of PARD3, PARD6, and an atypical protein kinase C (aPKC) regulate several aspects of neuronal migration.[review] PMID: 24243103
    25. Our results suggest that PAR-3 has a role in the clinical aggressiveness of cell Renal Cell Carcinoma PMID: 24136590
    26. Data indicate that both tumor focality and Par3/Par6/atypical protein kinase C (APKC) expression were significantly associated with tumor recurrence. PMID: 21549621
    27. Suggest that loss of Par3 promotes metastatic behaviour of ErbB2-induced breast tumour epithelial cells by decreasing cell-cell cohesion. PMID: 23263278
    28. Par3 is identified as a regulator of signaling pathways relevant to invasive breast cancer. PMID: 23153534
    29. A VE-cadherin-PAR3-alpha-catenin complex regulates the Golgi localization and activity of cytosolic phospholipase A(2)alpha in endothelial cells.( PMID: 22398721
    30. genetic association studies in a Chinese Han population: Data suggest that 6 SNPs in PARD3 (rs2496720, rs2252655, rs3851068, rs118153230, rs10827337, rs12218196) are associated with neural tube defects/anencephaly in this population. PMID: 22447895
    31. The scaffolding adaptor GAB1 interacts with two polarity proteins, PAR1 and PAR3. PMID: 22883624
    32. Changes in cell polarity proteins Par-3 and PP-1 are associated with altered expression and assembly of tight junction proteins claudin-2, -3, -5 and -7 and ZO-1, causing paracellular leakage in active coeliac disease. PMID: 21865402
    33. there was no association of MAGI2 and PARD3 with IBD. PMID: 21515326
    34. Factors-derived from preeclapsia placentas not only interrupt junction protein VE-cadherin distribution, but also perturb polarity protein PARD-3 expression and distribution in vascular endothelium PMID: 20959641
    35. Data show that DDR1 coordinates the Par3/Par6 cell-polarity complex through its carboxy terminus, binding PDZ domains in Par3 and Par6. PMID: 21170030
    36. Par3 controls epithelial spindle orientation by aPKC-mediated phosphorylation of apical Pins. PMID: 20933426
    37. The results suggest that Par3 directly interacts with FAK and PI3-kinase, enhancing their activities for polarized cell migration. PMID: 20191563
    38. Data show that Aurora A interacts directly with the atypical protein kinase C binding domain of Par3 and phosphorylates it at serine 962. PMID: 19812038
    39. findings demonstrated that G-protein-activated phospholipase C-beta interacts with cell polarity proteins Par3 and Par6 to form protein complexes and to mediate downstream signal transduction PMID: 15782111
    40. fine-tuning mechanism of Par3 in epithelial tight junction assembly controlled by the EGF receptor-SFK signaling pathway. PMID: 17053785
    41. We provide evidence for the existence of a distinct PAR protein complex in endothelial cells. Both PAR-3 and PAR-6 associate directly with the adherens junction protein vascular endothelial cadherin (VE-cadherin). PMID: 17057644
    42. Par3 is a novel component of the DNA-PK complex that implicates an unexpected link of cell polarity to double-strand DNA break repair. PMID: 17287830
    43. Because Numb interacts with the aPKC binding partner PAR-3, we propose a model in which polarized Numb phosphorylation contributes to cell migration by directing integrin endocytosis to the leading edge PMID: 17609107
    44. Two polarity proteins, Par3 and Par6, are also required for EC lumen and tube formation, as they establish EC polarity through their association with Cdc42 and atypical PKC. PMID: 18319301
    45. identified 10 potential novel binding proteins of PDZ domains of Par3 in Jurkat cells PMID: 18685789
    46. Results suggest that deletion and reduced expression of PARD3 may be a novel mechanism that drives the progression of ESCC. PMID: 19503097
    47. Cell polarity factor Par3 binds SPTLC1 and modulates monocyte serine palmitoyltransferase activity and chemotaxis PMID: 19592499
    48. Pard-3 and AS3 genes are mutationally inactivated in prostate cancer cells. PMID: 19737411

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  • 相关疾病：
    Neural tube defects (NTD)
  • 亚细胞定位：
    Cytoplasm. Endomembrane system. Cell junction. Cell junction, tight junction. Cell junction, adherens junction. Cell membrane. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    PAR3 family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 16051

    OMIM: 182940

    KEGG: hsa:56288

    STRING: 9606.ENSP00000363921

    UniGene: Hs.131489