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PDE8A Antibody

  • 货号：
    CSB-PA525315LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA525315LA01HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA525315LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PDE8A Polyclonal antibody
  • Uniprot No.：
    O60658
  • 基因名：
    PDE8A
  • 别名：
    5''-cyclic phosphodiesterase 8A antibody; cAMP specific cyclic nucleotide phosphodiesterase 8A antibody; FLJ16150 antibody; High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A antibody; High affinity cAMP-specific and IBMX-insensitive 3'' antibody; HsT19550 antibody; PDE 8A antibody; PDE8A antibody; PDE8A_HUMAN antibody; Phosphodiesterase 8A antibody; Phosphodiesterase8A antibody; Weakly similar to 3'5' cyclic nucleotide phosphodiesterase antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A protein (596-829AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，PDE8A Antibody (CSB-PA525315LA01HU)，的标记方式是Non-conjugated。对于PDE8A Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA525315LB01HU PDE8A Antibody, HRP conjugated ELISA
    FITC CSB-PA525315LC01HU PDE8A Antibody, FITC conjugated
    Biotin CSB-PA525315LD01HU PDE8A Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development. Binding to RAF1 reduces RAF1 'Ser-259' inhibitory-phosphorylation and stimulates RAF1-dependent EGF-activated ERK-signaling. Protects against cell death induced by hydrogen peroxide and staurosporine.
  • 基因功能参考文献：
    1. SNPs within genes SCHIP1 and PDE8A were associated with measures of facial size in both the GWAS and replication cohorts and passed a stringent genomewide significance threshold adjusted for multiple testing of 34 correlated traits. For both SCHIP1 and PDE8A, we demonstrated clear expression in the developing mouse face by both whole-mount in situ hybridization and RNA-seq PMID: 27560698
    2. PDE8A supports HIV-1 replication in macrophages PMID: 25295610
    3. a novel and critical role for phosphodiesterases in moderating local concentrations of cAMP in microdomains and signal resetting PMID: 25229150
    4. PDE8A can bind tightly to Raf-1, regulate Raf-1 phosphorylation on S259, and, in so doing, regulate the cross-talk node whereby cAMP exerts an inhibitory effect on Raf-1 signaling, retarding subsequent ERK phosphorylation and activation. PMID: 23509299
    5. PDE8 activity can be modulated by a kinase PMID: 22673573
    6. Polymorphism in PDE8A affects HIV-1 replication in primary macrophages. PMID: 21920574
    7. Comparison of enzymatic characterization and gene organization of PDE8B and PDE8A. PMID: 12681444
    8. in vitro and in vivo experiments demonstrated that the association with IkappaB greatly enhanced the enzyme activity of PDE8A1 PMID: 15596729
    9. kinetic properties of the highly active PDE8A1 catalytic domain prepared from refolding and its crystal structures in the unliganded and 3-isobutyl-1-methylxanthine (IBMX) bound forms at 1.9 and 2.1 A resolutions PMID: 18983167
    10. The findings exclude a significant role for PDE8A as a PCOS candidate gene, and as a Las major determinant of androgen levels in women. PMID: 19482904

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  • 蛋白家族：
    Cyclic nucleotide phosphodiesterase family, PDE8 subfamily
  • 组织特异性：
    Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.
  • 数据库链接：

    HGNC: 8793

    OMIM: 602972

    KEGG: hsa:5151

    STRING: 9606.ENSP00000311453

    UniGene: Hs.9333