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PDK1 Antibody

  • 货号：
    CSB-PA003729
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of Hela cells using PDK1 Polyclonal Antibody
    • Western Blot analysis of HuvEc cells using PDK1 Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    Q15118
  • 基因名：
  • 别名：
    [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody; HGNC:8809 antibody; Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody; PDH kinase 1 antibody; Pdk1 antibody; PDK1_HUMAN antibody; Pyruvate dehydrogenase kinase isoform 1 antibody; Pyruvate dehydrogenase kinase, isoenzyme 1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human PDK1.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    ELISA 1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
  • 基因功能参考文献：
    1. miR-375 and its probable PDK1 target may be utilized for the management of Kidney cancer. PMID: 30098579
    2. The importance of PDK1 in tumor growth and progression.A role of PDK1 in tumor microenvironment.[review] PMID: 28473254
    3. MiR-138 inhibits glycolysis but promotes mitochondrial respiration through directly targetting PDK1, and that contributes to cardiac cells' survival. PMID: 28899927
    4. Study shows higher expression level of PDK1 in non-small cell lung cancer (NSCLC) and its promoter region targeted by miR- 145. PMID: 28661070
    5. These results indicate that the immunohistochemistry analysis of the protein expression of PDK1, PHD3, and HIF-1alpha defines the hypoxic status of Neuroblastoma tumors. PMID: 29117193
    6. The pyruvate dehydrogenase kinases (PDKs) PDK1 and PDK3 are direct targets of KDM4A and E2F1 and modulate the switch between glycolytic metabolism and mitochondrial oxidation. PMID: 27626669
    7. dicumarol potently inhibited the kinase activity of PDK1, shifted the glucose metabolism from aerobic glycolysis to oxidative phosphorylation, generated a higher level of reactive oxygen species (ROS), attenuated the mitochondrial membrane potential (MMP), induced apoptosis, and reduced cell viability in vitro. PMID: 28617852
    8. miR-379 could function as a tumour-suppressing miRNA via targeting PDK1 in osteosarcoma. PMID: 27781416
    9. These results also suggest that inhibition of HIF-1a with 2-MeOE2 sensitizes radioresistant melanoma cells 435R to X-ray irradiation through targeting the glycolysis that is regulated by PDK1 PMID: 28339028
    10. PDK1 is frequently upregulated in primary nasopharyngeal carcinoma and may serve as a prognostic marker. PMID: 28029432
    11. A new function for PDK1 in metabolic reprogramming, which could be used to indicate the prognosis of Non small cell lung cancer and provide targeted therapeutic strategy for clinical treatment. PMID: 27878287
    12. Our results demonstrated that down-regulation of SDHB and up-regulation of PDK1 may be novel biomarkers for predicting advanced tumor progression and unfavorable prognosis in recurrent nasopharyngeal carcinoma patients PMID: 26547584
    13. n the conditon of miR- 128b over-expression, we also observed spontaneous inactivation of the Akt/NF-kappaB signalling, implying PDK1 was a potential regulator of this pathway. In conclusion, our study shed some novel light on miR-128b-PDK1/Akt/NF-kappaB axis onGastric cancer (GC) progression PMID: 26949090
    14. Dichloroacetate, an Iihibitor of PDK1, can reverse the mitochondrial suppression of renal cell carcinoma and decrease HIF transcriptional activity, decreasing tumor growth and angiogenesis. PMID: 26433571
    15. Tergeting PDK1 with dichloroacetophenone inhibited acute myeloid leukemia cell growth via multiple signaling pathways. PMID: 26593251
    16. PDK1 was specifically required for metabolic adaptation to nutrient limitation and hypoxia. PMID: 26365179
    17. High expression of PDK1 is associated with Colon Cancer. PMID: 26439504
    18. both PDK 1 and 2 isoforms are overexpressed in cutaneous melanoma compared to nevi, this expression being associated with the expression of the mTOR pathway effectors and independent of the BRAF mutational status PMID: 25976231
    19. Lin28A and Lin28B enhance, whereas let-7 suppresses, aerobic glycolysis via targeting pyruvate dehydrogenase kinase 1, or PDK1. PMID: 25301052
    20. Follow-up replication analyses in up to an additional 21,345 participants identified three new fasting plasma glucose loci reaching genome-wide significance in or near PDK1-RAPGEF4, KANK1, and IGF1R. PMID: 25187374
    21. NOR1 expression causes apoptosis of tumor cells in hypoxia by altering the expression of PDK1 expression and mitochondrial Bax-Bcl2 balance thus suppress tumor cell adaptation to hypoxia PMID: 24788728
    22. Accordingly, elevated levels of PDK1, PDK3, and PKM2 and reduced PK activity could be observed in iPSCs and human embryonic stem cells in the undifferentiated state PMID: 24123565
    23. High expression of pyruvate dehydrogenase kinase-1 is associated with gastric cancer. PMID: 23135628
    24. post-mortem brain extracts from patients with Alzheimer disease exhibited a decrease in PDK1 expression compared with nondemented patients. PMID: 22948140
    25. Pyruvate dehydrogenase kinase 1 is an important regulator of pyruvate dehydrogenase in clonal pancreatic beta-cells. PMID: 20415663
    26. mRNA levels not changed in skeletal muscle during fasting PMID: 14966024
    27. In summary, PDK activity is decreased after a high-fat diet that is rich in n-3 fatty acids, although PDHa activity was unaltered. PMID: 15591305
    28. HIF-1 also actively suppresses metabolism through the tricarboxylic acid cycle (TCA) by directly trans-activating the gene encoding pyruvate dehydrogenase kinase 1 (PDK1). PMID: 16517405
    29. Distinct structural mechanisms for inhibition of PDK1 by AZD7545, dichloroacetate, and radicol. PMID: 17683942
    30. HIF regulation of PDK-1 has a key role in maintaining lactate production in human cancer and that the investigation of PDK-1 inhibitors should be investigated for antitumour effects PMID: 18542064
    31. Data show that PDK1 and HIF prolyl hydroxylase 3 expressions are lowest in children of chronic mountain sickness fathers at altitude. PMID: 18954447

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  • 亚细胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    PDK/BCKDK protein kinase family
  • 组织特异性：
    Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.
  • 数据库链接：

    HGNC: 8809

    OMIM: 602524

    KEGG: hsa:5163

    STRING: 9606.ENSP00000282077

    UniGene: Hs.470633