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PHACTR1 Antibody

  • 货号：
    CSB-PA017880LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, Rat brain tissue, Mouse brain tissue
      All lanes: PHACTR1 antibody at 6µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 67, 55 kDa
      Observed band size: 67 kDa
    • IHC image of CSB-PA017880LA01HU diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA017880LA01HU diluted at 1:400 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of A549 cells with CSB-PA017880LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PHACTR1 Polyclonal antibody
  • Uniprot No.：
    Q9C0D0
  • 基因名：
    PHACTR1
  • 别名：
    PHACTR1 antibody; KIAA1733 antibody; RPEL1 antibody; Phosphatase and actin regulator 1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human Phosphatase and actin regulator 1 protein (263-387AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，PHACTR1 Antibody (CSB-PA017880LA01HU)，的标记方式是Non-conjugated。对于PHACTR1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA017880LB01HU PHACTR1 Antibody, HRP conjugated ELISA
    FITC CSB-PA017880LC01HU PHACTR1 Antibody, FITC conjugated
    Biotin CSB-PA017880LD01HU PHACTR1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Binds actin monomers (G actin) and plays a role in multiple processes including the regulation of actin cytoskeleton dynamics, actin stress fibers formation, cell motility and survival, formation of tubules by endothelial cells, and regulation of PPP1CA activity. Involved in the regulation of cortical neuron migration and dendrite arborization.
  • 基因功能参考文献：
    1. our study suggests that several PHACTR1 and SLC22A3 gene polymorphisms may exert a protective effect against the CAD in the Chinese Han male population. PMID: 27893421
    2. PHACTR1 is a key atherosclerosis candidate gene since it is regulated by atherogenic stimuli in macrophages and endothelial cells PMID: 27187934
    3. Stimulation of Slack K(+) channels alters mass at the plasma membrane by triggering dissociation of Phactr-1. PMID: 27545877
    4. CRISPR-edited stem cell-derived endothelial cells demonstrate rs9349379, a common SNP in the 3 intron of the PHACTR1 gene, regulates expression of endothelin 1 (EDN1), a gene located 600 kb upstream of PHACTR1. The known physiologic effects of EDN1 on the vasculature may explain the pattern of risk for the five associated vascular diseases. PMID: 28753427
    5. To evaluate the role of two polymorphisms (rs2026458 and rs9349379) of the PHACTR1 gene in the susceptibility to the risk of developing premature coronary artery disease (CAD) in the Mexican population. Results suggest that the PHACTR1 rs9349379 polymorphism plays an important role in the risk of developing premature CAD in the Mexican population. PMID: 27517945
    6. the expression of PHACTR1 by genotypes in primary human fibroblasts showed higher expression in rs9349379-A carriers . Phactr1 knockdown in zebrafish resulted in dilated vessels indicating subtle impaired vascular development PMID: 27792790
    7. There was no significant association between the presence of risk alleles of rs12526453 and coronary heart disease in an Iranian population. PMID: 28287809
    8. our result highlighted the pivotal role of phactr-1 protein in the pathogenesis of atherosclerosis. PMID: 26362351
    9. The rs12526453 CC homozygotes (previously associated with increased risk of myocardial infarction) showed, in 2 independent samples, better long-term survival. PMID: 26086777
    10. GWAS analysis of 1,393 cervical artery dissection cases and 14,416 controls showed that the rs9349379[G] allele was associated with lower risk. This was confirmed independent follow-up samples. PMID: 25420145
    11. a novel signaling route whereby TGF-beta silences the expression of miR-584, resulting in enhanced PHACTR1 expression, and further leading to actin rearrangement and breast cancer cell migration. PMID: 23479725
    12. 3 loci from related cardiovascular genomewide studies were significant: PHACTR1 in large-vessel disease (P=2.63e(-6)), PITX2 in cardioembolic stroke (P=4.78e(-8)), and ZFHX3 in cardioembolic stroke (P=5.50e(-7)). PMID: 23042660
    13. Results from this GWAS support a central role of PHACTR1 in CAD susceptibility irrespective of lifestyle and ethnic divergences. PMID: 22745674
    14. Phactr-1 is a key component in the angiogenic process PMID: 21939755
    15. Findings suggest that PHACTR-1 is likely to be a key regulator of endothelial cell function properties. PMID: 21798305
    16. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 20738937
    17. Observational study of gene-disease association. (HuGE Navigator) PMID: 20971364
    18. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
    19. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 19198609

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  • 亚细胞定位：
    Cytoplasm. Cell junction, synapse. Nucleus.
  • 蛋白家族：
    Phosphatase and actin regulator family
  • 组织特异性：
    Detected in umbilical vein endothelial cells.
  • 数据库链接：

    HGNC: 20990

    OMIM: 608723

    KEGG: hsa:221692

    STRING: 9606.ENSP00000329880

    UniGene: Hs.436996