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  4. PHLPP1 Antibody

PHLPP1 Antibody

  • 货号：
    CSB-PA555844
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA555844(PHLPP1 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    O60346
  • 基因名：
  • 别名：
    hSCOP antibody; KIAA0606 antibody; PH domain and leucine rich repeat protein phosphatase antibody; PH domain leucine rich repeat containing protein phosphatase antibody; PH domain leucine rich repeat protein phosphatase antibody; PH domain leucine-rich repeat-containing protein phosphatase 1 antibody; PH domain-containing family E member 1 antibody; PHLP1_HUMAN antibody; PHLPP antibody; Phlpp1 antibody; Pleckstrin homology domain containing family E (with leucine rich repeats) member 1 antibody; Pleckstrin homology domain containing family E protein 1 antibody; Pleckstrin homology domain-containing family E member 1 antibody; PLEKHE 1 protein antibody; PLEKHE1 antibody; SCN circadian oscillatory protein antibody; SCOP antibody; Suprachiasmatic nucleus circadian oscillatory protein antibody; Suprachiasmatic nucleus circadian protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Fusion protein of Human PHLPP1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    IHC 1:25-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA. Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylates RAF1 inhibiting its kinase activity. May act as a negative regulator of K-Ras signaling in membrane rafts. Involved in the hippocampus-dependent long-term memory formation. Involved in circadian control by regulating the consolidation of circadian periodicity after resetting. Involved in development and function of regulatory T-cells.
  • 基因功能参考文献：
    1. Data suggest a tumor suppressor role of Xist in inhibiting AKT activation via regulation of non-X-chromosome gene PHLPP1 expression. PMID: 27248326
    2. Low PHLPP expression is associated with gallbladder cancer. PMID: 28212545
    3. nickel exposure results in DNMT3b induction and MEG3 promoter hypermethylation and expression inhibition, further reduces its binding to c-Jun and in turn increasing c-Jun inhibition of PHLPP1 transcription, leading to the Akt/p70S6K/S6 axis activation, and HIF-1alpha protein translation, as well as malignant transformation of human bronchial epithelial cells. PMID: 28263966
    4. Studies indicate that two PHLPP isozymes, PHLPP1 and PHLPP2, were identified in a search for phosphatases that dephosphorylate Akt, and thus suppress growth factor signaling. PMID: 27913677
    5. During LPS induced macrophage inflammatory response, PHLPP levels in macrophages are depleted through the inhibition of SP1 dependent transcriptional regulation. PMID: 28322791
    6. Abnormal PHLPP, FoxO3a, and RAD51 protein expressions may be involved in the development of high- and low-grade ovarian serous adenocarcinomas, suggesting common molecular pathways. PMID: 28139510
    7. this study shows that PHLPP1 played a suppression role in inflammatory response of glioma PMID: 26971226
    8. Increased PHLPP expression is associated with pancreatic cancer. PMID: 26760962
    9. our results suggest that PHLPP1 plays a crucial role in sacral chordoma PMID: 26823799
    10. high levels of PHLPP might reflect a less aggressive lung adenocarcinoma phenotype and predict better survival in patients with lung adenocarcinoma. PMID: 26463718
    11. PHLPP1, along with miR-522, promoted tumor cell growth in glioblastoma cells. PMID: 25776496
    12. Suppression of PHLPP1 by DNA methylation contributes to melanoma development and progression. PMID: 24121273
    13. PHLPP1 is a binding protein for Mst1 and it modulates the Hippo pathway by dephosphorylating Mst1 at the inhibitory Thr(387) of Mst1. PMID: 24393845
    14. our results reveal WDR48 and USP12 as novel PHLPP1 regulators and potential suppressors of tumor cell survival. PMID: 24145035
    15. The overall survival time and relapse-free survival time in PHLPP1-positive gastric cancer patients were significantly longer than in PHLPP1-negative patients. PMID: 24037758
    16. A hypoxia-induced decrease of PHLPP expression is attenuated by knocking down HIF1alpha but not HIF2alpha. PMID: 24061475
    17. Data indicate that binding affinities for the PH domains of Akt and PHLPP1 were greater than for other PH domain-containing proteins, which may underlie the preferential recruitment of these proteins to membranes containing tocopherols. PMID: 23512990
    18. we found that SGT1 was able to regulate the stability of PHLPP1, which is the direct phosphatase for Akt ser473 phosphorylation PMID: 23440515
    19. USP46-mediated stabilization of PHLPP and the subsequent inhibition of Akt. PMID: 22391563
    20. AG490 time-dependently down-regulated the protein expression of PHLPP and induced apoptosis of K562 cells PMID: 22931649
    21. Studies suggest that the effects of PHLPP1 deletion on prostate carcinogenesis may be explained by the strong dependence of prostate cancer on phosphoinositide 3-kinase (PI3K)/Akt signalling. PMID: 22340730
    22. Loss of PHLPP1 is associated with chronic lymphocytic leukemia. PMID: 22237780
    23. a novel USP1-PHLPP1-Akt signaling axis, and decreased USP1 level in lung cancer cells may play an important role in lung cancer progress. PMID: 22426999
    24. PHLPP1 has tumor suppressive activity and might represent a therapeutic or diagnostic tool for pancreatic ductal adenocarcinoma. PMID: 22044669
    25. show for the first time a significant disruption of all three members of the PTEN-NHERF1-PHLPP1 tumor suppressor network in high-grade tumors, correlating with Akt activation and patient's abysmal survival PMID: 21804599
    26. PHLPP as a novel tumor suppressor. PMID: 22144674
    27. Knockdown of Scribble (Scrib) results in redistribution of PHLPP1 from the membrane to the cytoplasm and an increase in Akt phosphorylation. PMID: 21701506
    28. codeletion of PTEN and PHLPP1 in patient samples is highly restricted to metastatic disease and tightly correlated to deletion of TP53 and PHLPP2 PMID: 21840483
    29. Increased abundance of PHLPP-1, production of which is regulated by insulin, may represent a new molecular defect in insulin-resistant states such as obesity. PMID: 21461637
    30. the cellular localization of beta-TrCP1 is altered in glioblastoma, resulting in dysregulation of PHLPP1 and other substrates such as beta-catenin. PMID: 21454620
    31. a compensatory feedback regulation in which the activation of Akt is inhibited by up-regulation of PHLPP through mTOR, and this mTOR-dependent expression of PHLPP subsequently determines the rapamycin sensitivity of cancer cells. PMID: 21177869
    32. Reduced expression of the tumor suppressor PHLPP1 enhances the antiapoptotic B-cell receptor signal in chronic lymphocytic leukemia B-cells. PMID: 20861921
    33. Aberrantly expressed UCH-L1 boosts signaling through the Akt pathway by downregulating the antagonistic phosphatase PHLPP1, an event that requires its de-ubiquitinase activity PMID: 20574456
    34. In the striatum, reduced levels of PHLPP1 can contribute to maintain high levels of activated Akt that may delay cell death and allow the recovery of neuronal viability after mutant huntingtin silencing PMID: 19745829
    35. The PHLPP1 dephosphorylate Mst1 on the T387 inhibitory site, which activate Mst1 and its downstream effectors p38 and JNK to induce apoptosis. PMID: 20513427
    36. Mechanism to selectively terminate Akt-signaling pathways through the differential inactivation of specific Akt isoforms by specific PHLPP isoforms. PMID: 17386267
    37. PHLPP controls the cellular levels of PKC by specifically dephosphorylating the hydrophobic motif, thus destabilizing the enzyme and promoting its degradation. PMID: 18162466
    38. Study focuses on the function of PHLPP1 and PHLPP2 in modulating signaling by Akt and PKC. PMID: 18511290
    39. Loss of PHLPP expression is associated with colon cancer. PMID: 19079341
    40. demonstrate a key role for beta-TrCP in controlling the level of PHLPP1, and activation of Akt negatively regulates this degradation process. PMID: 19797085

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  • 亚细胞定位：
    Cytoplasm. Membrane; Peripheral membrane protein. Nucleus. Note=In colorectal cancer tissue, expression is concentrated at the lateral membrane of epithelial cells.; [Isoform 2]: Cell membrane.
  • 组织特异性：
    In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 78% of tested tumors (at pro
  • 数据库链接：

    HGNC: 20610

    OMIM: 609396

    KEGG: hsa:23239

    STRING: 9606.ENSP00000262719

    UniGene: Hs.465337