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PIK3C3 Antibody

  • 货号：
    CSB-PA909570
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA909570(PIK3C3 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using CSB-PA909570(PIK3C3 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    Q8NEB9
  • 基因名：
  • 别名：
    hVps34 antibody; MGC61518 antibody; Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody; Phosphatidylinositol 3 kinase class 3 antibody; Phosphatidylinositol 3 kinase p100 subunit antibody; Phosphatidylinositol 3-kinase catalytic subunit type 3 antibody; Phosphatidylinositol 3-kinase p100 subunit antibody; Phosphoinositide 3 kinase class 3 antibody; Phosphoinositide-3-kinase class 3 antibody; PI3 kinase type 3 antibody; PI3-kinase type 3 antibody; PI3K type 3 antibody; Pik3c3 antibody; PK3C3_HUMAN antibody; PtdIns 3 kinase type 3 antibody; PtdIns-3-kinase type 3 antibody; Vps 34 antibody; Vps34 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthetic peptide of Human PIK3C3
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.
  • 基因功能参考文献：
    1. Vps34 stimulates tumor development mainly through PKC-delta- activation of p62. PMID: 28846113
    2. Here we show that a putative fifth subunit, nuclear receptor binding factor 2 (NRBF2), is a tightly bound component of the class III phosphatidylinositol 3-kinase complex I that profoundly affects its activity and architecture. NRBF2 is a homodimer and drives the dimerization of the larger PI3KC3-C1 complex, with implications for the higher-order organization of the preautophagosomal structure. PMID: 27385829
    3. This study reveals NRBF2 as a critical molecular switch of PtdIns3K and autophagy activation, and its on/off state is precisely controlled by MTORC1 through phosphorylation. PMID: 28059666
    4. Atg38 and its human ortholog NRBF2, accessory components of complex I consisting of Vps15-Vps34-Vps30/Atg6-Atg14 (yeast) and PIK3R4/VPS15-PIK3C3/VPS34-BECN1/Beclin 1-ATG14 (human), were characterized. PMID: 27630019
    5. p300-dependent VPS34 acetylation/deacetylation is the physiological key to VPS34 activation, which controls the initiation of canonical autophagy and of non-canonical autophagy in which the upstream kinases of VPS34 can be bypassed. PMID: 28844862
    6. Low VPS34 expression is associated with cancer. PMID: 28157699
    7. Arf tumor suppressor as a new transcriptional target of nuclear EGFR and highlight Vps34 as an important regulator of the nuclear EGFR/Arf survival pathway. PMID: 26686095
    8. Vps34 has a previously unknown role in regulating Rab7 activity and late endosomal trafficking. PMID: 27793976
    9. High expression of VPS34 promoted GRP78 transcription by modulating ATF6. VPS34 could enhance GRP78 protein stability. PMID: 28038917
    10. These results establish Vps34 as an essential determinant of both short-term and long-term canonical GPCR signaling. PMID: 27821547
    11. Study identifies a key role of Cul3-KLHL20 in autophagy termination by controlling autophagy-dependent turnover of ULK1 and VPS34 complex subunits and reveals the pathophysiological functions of this autophagy termination mechanism. PMID: 26687681
    12. cis-unsaturated fatty acids require neither BECN1 nor PIK3C3 to stimulate the autophagic flux PMID: 25714112
    13. tubulation requires mTOR activity, and we identified two direct mTOR phosphorylation sites on UVRAG (S550 and S571) that activate VPS34. PMID: 26139536
    14. High expression of PI3K core complex genes is associated with poor prognosis in chronic lymphocytic leukemia. PMID: 25840748
    15. reveal a novel function of GABP in the regulation of autophagy via transcriptional activation of the BECN1-PIK3C3 complex PMID: 25046113
    16. Data indicte that Compound 31 constitutes an optimized class III phosphoinositide 3-kinase Vps34 inhibitor that could be used to investigate cancer biology. PMID: 25402320
    17. VPS34-IN1 will provide a useful tool to decipher the kinase-dependent functions of Vps34, with acute changes in SGK3 phosphorylation and subcellular localization being new biomarkers of Vps34 activity. PMID: 25395352
    18. DNA damage regulates Vps34 complexes and its downstream mechanisms, including autophagy and receptor endocytosis, through SCF (Skp1-Cul1-F-box)-mediated ubiquitination and degradation PMID: 25593308
    19. Insulin can spatially regulate VPS34 activity through Src-mediated tyrosine phosphorylation. PMID: 24582588
    20. Ric-8A co-localized with Vps34 at the midbody. PMID: 24466196
    21. A mechanistic link between amino acid starvation and autophagy induction via the direct activation of the autophagy-specific PIK3C3 kinase. PMID: 24013218
    22. NRBF2 regulates macroautophagy as a component of Vps34 Complex I. PMID: 24785657
    23. Via direct interaction with the class III PI-3-kinase (PI3KC3)/Beclin1, DEDD activated autophagy and induced the degradation of Snail and Twist, two master regulators of EMT PMID: 22719072
    24. Results describe PKD as a novel Vps34 kinase that functions as an effecter of autophagy under oxidative stress. PMID: 22095288
    25. conclude that Slamf1 recruits a subset of Vps34-associated proteins, which is involved in membrane fusion and NOX2 regulation PMID: 22493499
    26. Though dispensable for autophagy induction, transgenic Vps34 is a critical regulator of naive T cell homeostasis, modulating interleukin (IL)-7 receptor alpha trafficking, signaling, and recycling. PMID: 22021616
    27. Class III PI-3-kinase activates phospholipase D in an amino acid-sensing mTORC1 pathway PMID: 22024166
    28. Pik3c3 is essential for central nervous system neuronal homeostasis and Pik3c3 deletion; CaMKII-Cre transgenic mouse is a useful model for studying pathological changes in progressive forebrain neurodegeneration. PMID: 20955765
    29. Coimmunoprecipitation assay indicated that hepatitis C virus NS4B formed a complex with human Rab5 and Vps34, supporting the notion that Rab5 and Vps34 are involved in NS4B-induced autophagy. PMID: 21835792
    30. Activation of mTOR by leucine or insulin upregulated hVps34. PMID: 21702994
    31. 14-3-3zeta proteins are shown as a negative regulator of autophagy through regulation of a key component of early stages of the autophagy pathway, such as hVps34. PMID: 20885446
    32. a critical role of the Rubicon RUN domain in PI3KC3 and autophagy regulation PMID: 21062745
    33. A specific sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1 regulates both receptor degradation and cytokinesis, whereas ATG14L, a PI3K-III subunit involved in autophagy, is not required. PMID: 20643123
    34. A PIK3C3 promoter variant (rs3813065/-442 C/T) in an independent multiancestral cohort of 478 systemic lupus erythematosus cases and 522 controls, was examined. PMID: 20671926
    35. Data show that knockdown of Vps34 reduces gossypol-induced autophagy in both MCF-7 human breast adenocarcinoma and HeLa cell lines. PMID: 20529838
    36. A phylogenetic study revealing co-evolution of myotubularins phosphoinositides phosphatases with PI 3-kinase class III complex PMID: 18774718
    37. was expressed in cancer tissues at 11 times the level of that found in normal tissue; findings suggest that activation of the PI3K-AKT signal pathway is associated with oral carcinogenesis PMID: 19887755
    38. Results describe how Mycobacterium tuberculosis toxin lipoarabinomannan causes phagosome maturation arrest, interfering with a calcium/calmodulin phosphatidylinositol (PI)3 kinase hVPS34 cascade. PMID: 12925680
    39. Data identify rab7 as an important regulator of late endosomal VPS34 function and link rab7 to the regulation of phosphatidylinositol 3'-kinase cycling between early and late endosomes. PMID: 14617358
    40. A promoter mutation in a PI regulator affecting the binding of a POU-type transcription factor may be involved in BD and SZ in a subset of patients. PMID: 15121481
    41. hVps34 is a nutrient-regulated lipid kinase that integrates amino acid and glucose inputs to mTOR and S6K1 PMID: 16049009
    42. Amino acids mediate mTOR activation by signaling through class 3 PI3K, hVps34. PMID: 16176982
    43. Results argue against a role for Beclin 1 as an essential chaperone or adaptor for hVps34 in normal vesicular trafficking, and they support the hypothesis that Beclin 1 functions mainly to engage hVps34 in the autophagic pathway. PMID: 16390869
    44. There is a connection between Beclin 1-associated Class III PI3K/Vps34-dependent autophagy, but not VPS, function and the mechanism of Beclin 1 tumor suppressor action in human breast cancer cells. PMID: 16874027
    45. study indicates that hVps34 and its product PI(3)P are involved in endosome to Golgi transport of ricin, and that SNX2 and SNX4 are likely to be effectors in this pathway PMID: 17319803
    46. the lipid kinase activity of Vps34 has a role in resveratrol-induced apoptosis and in the formation of autophagolysosomes PMID: 18048384
    47. our results do support PIK3C3 play a significant role in the etiology of schizophrenia PMID: 18420347
    48. SopB mediates PI(3)P production on the SCV indirectly through recruitment of Rab5 and its effector Vps34. PMID: 18725540
    49. hVps34 activity is regulated through its interactions with hVps15, but is independent of Ca2+/CaM. PMID: 18957027

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  • 亚细胞定位：
    Midbody. Late endosome. Cytoplasmic vesicle, autophagosome.
  • 蛋白家族：
    PI3/PI4-kinase family
  • 组织特异性：
    Ubiquitously expressed, with a highest expression in skeletal muscle.
  • 数据库链接：

    HGNC: 8974

    OMIM: 602609

    KEGG: hsa:5289

    STRING: 9606.ENSP00000262039

    UniGene: Hs.464971