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PIWIL1 Antibody

  • 货号：
    CSB-PA571908
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA571908(PIWIL1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA571908(PIWIL1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    Q96J94
  • 基因名：
    PIWIL1
  • 别名：
    HIWI antibody; MIWI antibody; Piwi (Drosophila) like 1 antibody; PIWI antibody; Piwi homolog antibody; Piwi like 1 (drosophila) antibody; Piwi like 1 antibody; Piwi-like protein 1 antibody; PIWIL1 antibody; PIWL1_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Fusion protein of Human PIWIL1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:5000
    IHC 1:25-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Required to sequester RNF8 in the cytoplasm until late spermatogenesis; RNF8 being released upon ubiquitination and degradation of PIWIL1.; May be a negative developmental regulator.
  • 基因功能参考文献：
    1. piRNAs may function in gene silencing and gene modification upstream or downstream of oncogenes in cancer cell lines or cancer tissues. In addition to piRNAs, PIWI proteins can be used as biomarkers for prognosis, diagnosis and clinical evaluation and may be factors in cancer metastasis. PMID: 30226604
    2. Unlike most other Tudor domains TDRD2 preferentially recognizes an unmethylated arginine-rich sequence from PIWIL1. PMID: 29118143
    3. HENMT1 and PIWI proteins are expressed in a germ-cell-specific manner and required for transposon control. PMID: 28676534
    4. A statistically significantly higher level of PIWIL1 and PIWIL2 was found in IDC compared to mastopathy samples (p
    5. Expression of PIWIL1 in the cancer tissue was higher than that in the corresponding adjacent tissue. High PIWIL1 expression suggests a poor prognosis for colorectal cancer patients. PMID: 28634417
    6. PIWIL3 and PIWIL4 genes showed prognostic relevance. 306 gene targets exhibited reciprocal relationship with piRNA expression PMID: 27177224
    7. Findings identify Piwi as a factor in human infertility and reveal its role in regulating the histone-to-protamine exchange during spermiogenesis. PMID: 28552346
    8. In blood serum, piR-823 is up-regulated, but with unsatisfactory analytical performance. Preliminary data indicate the promising diagnostic utility of urinary piR-823 in patients with RCC PMID: 27919963
    9. Overexpression of Hiwi significantly suppressed cell proliferation and cell migration and induced apoptosis in K562 cells. PMID: 25701408
    10. Hiwi upregulation enhanced chemosensitivity of Chronic Myeloid Leukemia cells to daunomycin, suppressed cell proliferation and promoted apoptosis. PMID: 25701955
    11. HIWI promotes the activity of breast cancer cells while depression of HIWI may induce apoptosis of breast cancer cells. PMID: 26847393
    12. Our study reveals a novel mechanism for PIWIL1 in tumorigenesis. PMID: 26317901
    13. PIWIL1 expression emerged as an independent prognostic marker in non-small cell lung cancer. PMID: 25742785
    14. Among patients with high let-7a expression, those with medium HIWI had an increased risk of death compared to those with low HIWI (HR = 2.62, 95% CI: 1.30-5.30). PMID: 25630839
    15. results suggested that Piwil1 caused the loss of PTEN expression through DNMT1-mediated PTEN hypermethylation PMID: 26056945
    16. Results show that HIWI is highly expressed in high-grade squamous intraepithelial lesions and cervical cancer tissues suggesting its participation in the carcinogenesis of cervical cancer. PMID: 25119492
    17. Our results indicate a reciprocal regulation between HIWI, HILI and some CSCs markers in colorectal cancer PMID: 26026091
    18. Evidence of an active PIWI-interacting small non-coding RNAs pathway in BC suggests that these small non-coding RNAs do exert transcriptional and post-transcriptional gene regulatory actions also in cancer cells. PMID: 25313140
    19. Hiwi downregulation mediated by shRNA reduce the proliferation and migration of HCC cells. PMID: 25370791
    20. These findings suggest that HIWI is an oncogene involved in the progression of glioma. PMID: 25269862
    21. Overexpression of hiwi promotes growth of breast cancer cells. PMID: 25292027
    22. IGFBP-5 may be a negative modulator of RASSF1C/ PIWIL1 growth-promoting activities. PMID: 25007054
    23. A important role of HIWI and DPPA2 in tumorigenesis and also in lymph node metastasis of tumor cells. PMID: 24532429
    24. detection of mitochondrial Piwi by colocalization using anti-Piwil1 and mitochondria organelle-specific protein antibodies in cancer cell lines PMID: 24602614
    25. Our study identifies surrogate DNA methylation markers for idiopathic infertility in peripheral blood and suggests that allele-specific DNA methylation differences at regulatory sites of genes are associated with disturbed spermatogenesis. PMID: 24524831
    26. associations were determined between the Piwi-like family member expression levels and clinicopathological parameters of ccRCC, suggesting a potential role for these genes/proteins in ccRCC diagnostics and tumorigenesis PMID: 24509249
    27. Polymorphism in PIWIL1 gene is associated with HBV-related hepatocellular carcinoma PMID: 23868705
    28. Findings suggest the role of RASSF1C/PIWIL1 proteins in initiation and progression of lung cancer. PMID: 22591718
    29. Expression of PIWIL1 was an independent prognostic factor in gastric cancer. PMID: 22670175
    30. Hiwi-associated DNA hyper-methylation with subsequent genetic and epigenetic changes favors a tumorigenic state PMID: 22438986
    31. HIWI may play a key role in hepatocellular carcinoma (HCC) proliferation and metastasis and can be a potential prognostic factor for HCC after curative resection PMID: 21989785
    32. A subgroup of more aggressive tumors can be identified by evaluating Hiwi level in lymph node metastatic cancer. PMID: 22261620
    33. Colorectal cancer patients with positive HIWI expression in adjacent non-cancerous tissue had statistically lower overall survival (OS) and disease free survival (DFS) compared with negative patients. PMID: 21933617
    34. In this review, the current state of knowledge on the structure, biogenesis and function of piRNA and their interactions with Piwi proteins is presented. PMID: 22235650
    35. HIWI expression is significantly higher in HCC tissue than in adjacent normal hepatic tissue. PMID: 21327579
    36. The human piwi-like 1 PAZ domain is a typical OB fold and specifically recognizes the 2nt 3 0 -OH end of the single-stranded RNA and buries the two 3 0 -terminal nucleotides in the hydrophobic cleft. PMID: 21465557
    37. The result of this study suggested that Hiwi may be a critical factor in glioma progression and could be used as a potential molecular marker for pathological diagnosis and prognosis evaluation for malignant gliomas. PMID: 21138738
    38. These results suggest that hiwi plays an important role in the oncogenesis and is a potential target for cancer angiogenesis PMID: 20204292
    39. The expression of HIWI in the cytoplasm of esophageal cancer cells is significantly associated with higher histological grade, clinical stage and poorer clinical outcome PMID: 19995427
    40. specifically expressed in both normal and malignant spermatogenic cells in a maturation stage-dependent pattern, in which it might function in germ cell proliferation PMID: 12037681
    41. results suggest that hiwi may be involved in the development of gastric cancer and is a potential target for cancer therapy PMID: 16287078
    42. High-level expression of Hiwi mRNA identifies soft-tissue sarcoma patients at high risk of tumor-related death. PMID: 16953229
    43. alterations in mRNA expression of Hiwi can increase the risk of tumour-related death in male ductal pancreatic carcinoma patients PMID: 18781170

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  • 相关疾病：
    Defects in PIWIL1 may be a cause of a disorder resulting in the absence of sperm (azoospermia) in the semen, leading to male infertility. Male sterility can be caused by defects in ubiquitination and degradation during late spermatogenesis.
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Argonaute family, Piwi subfamily
  • 组织特异性：
    Expressed in spermatocytes and spermatids. Also detected in prostate cancer (at protein level). Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis.
  • 数据库链接：

    HGNC: 9007

    OMIM: 605571

    KEGG: hsa:9271

    STRING: 9606.ENSP00000245255

    UniGene: Hs.405659