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POLDIP3 Antibody

  • 货号：
    CSB-PA871610LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate
      All lanes: POLDIP3 antibody at 3.2μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 47, 43 kDa
      Observed band size: 43 kDa
    • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA871610LA01HU at dilution of 1:100
    • Immunofluorescence staining of Hela cells with CSB-PA871610LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunoprecipitating POLDIP3 in Jurkat whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA871610LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA871610LA01HU (8μg) + Jurkat whole cell lysate (500μg)
      Lane 3: Jurkat whole cell lysate (10μg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) POLDIP3 Polyclonal antibody
  • Uniprot No.：
    Q9BY77
  • 基因名：
    POLDIP3
  • 别名：
    46 kDa DNA polymerase delta interaction protein antibody; DNA polymerase delta interacting protein 3 antibody; KIAA1649 antibody; P46 antibody; PDIP3_HUMAN antibody; PDIP46 antibody; POLDIP3 antibody; Polymerase (DNA-directed), delta interacting protein 3 antibody; Polymerase delta interacting protein 46 antibody; Polymerase delta-interacting protein 3 antibody; RNA-binding protein P46 antibody; S6K1 Aly/REF-like target antibody; SKAR antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Polymerase delta-interacting protein 3 protein (100-221AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，POLDIP3 Antibody (CSB-PA871610LA01HU)，的标记方式是Non-conjugated。对于POLDIP3 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA871610LB01HU POLDIP3 Antibody, HRP conjugated ELISA
    FITC CSB-PA871610LC01HU POLDIP3 Antibody, FITC conjugated
    Biotin CSB-PA871610LD01HU POLDIP3 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:20-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Involved in nuclear mRNA export; probably mediated by association with the TREX complex.
  • 基因功能参考文献：
    1. Altered expression of PDIP46 or its mutation may affect Pol delta functions in vivo, and thereby be a nexus for altered genomic stability. PMID: 26819372
    2. hnRNP-U inhibits TDP-43-mediated alterations in splicing of POLDIP3 mRNA. PMID: 25378556
    3. PDIP3 and ZC11A associate with the human TREX complex in an ATP-dependent manner and function in mRNA export. PMID: 22928037
    4. these findings establish critical and essential roles for SKAR in the regulation of mRNA translation of IFN-sensitive genes and induction of IFN-alpha biological responses. PMID: 25049393
    5. Alteration of POLDIP3 splicing associated with loss of function of TDP-43 in tissues affected with amyotrophic lateral sclerosis (ALS) . PMID: 22900096
    6. SKAR is a specific target of S6K1 and regulates cell growth. PMID: 15341740
    7. This study showed that PDIP46 aka SKAR, a protein possessing one RNA recognition motif (RRM) and being a protein partner of both the p50 subunit of DNA polymerase delta and p70 ribosomal protein S6 kinase 1 (S6K1). PMID: 16984396
    8. SKAR-mediated recruitment of activated S6K1 to newly processed mRNPs serves as a conduit between mTOR checkpoint signaling and the pioneer round of translation when cells exist in conditions supportive of protein synthesis. PMID: 18423201

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  • 亚细胞定位：
    Nucleus. Nucleus speckle. Cytoplasm. Note=Nucleocytoplasmic shuttling protein.
  • 数据库链接：

    HGNC: 23782

    OMIM: 611520

    KEGG: hsa:84271

    STRING: 9606.ENSP00000252115

    UniGene: Hs.505802