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PPP2R2B Antibody

  • 货号：
    CSB-PA018565LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA018565LA01HU diluted at 1:600 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of SH-SY5Y cells with CSB-PA018565LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • IHC image of CSB-PA018565LA01HU diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PPP2R2B Polyclonal antibody
  • Uniprot No.：
    Q00005
  • 基因名：
  • 别名：
    2ABB_HUMAN antibody; B55 beta antibody; Beta isoform of regulatory subunit B55 protein phosphatase 2 antibody; MGC24888 antibody; PP2A B55beta antibody; PP2A PR55B antibody; PP2A subunit B B beta isoform antibody; PP2A subunit B B55 beta isoform antibody; PP2A subunit B isoform B55 beta antibody; PP2A subunit B isoform B55-beta antibody; PP2A subunit B isoform beta antibody; PP2A subunit B isoform PR55 beta antibody; PP2A subunit B isoform PR55-beta antibody; PP2A subunit B isoform R2 beta antibody; PP2A subunit B isoform R2-beta antibody; PP2A subunit B PR55 beta isoform antibody; PP2A subunit B R2 beta isoform antibody; Ppp2r2b antibody; PR2AB beta antibody; PR2AB55 beta antibody; PR2APR55 beta antibody; PR52B antibody; PR55 beta antibody; Protein phosphatase 2 (formerly 2A) regulatory subunit B (PR 52) beta isoform antibody; Protein phosphatase 2 (formerly 2A) regulatory subunit B beta isoform antibody; Protein phosphatase 2 regulatory subunit B antibody; Protein phosphatase 2 regulatory subunit B beta isoform antibody; SCA 12 antibody; SCA12 antibody; Serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B beta isoform antibody; Serine/threonine protein phosphatase 2A neuronal isoform antibody; Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform protein (1-200AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，PPP2R2B Antibody (CSB-PA018565LA01HU)，的标记方式是Non-conjugated。对于PPP2R2B Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA018565LB01HU PPP2R2B Antibody, HRP conjugated ELISA
    FITC CSB-PA018565LC01HU PPP2R2B Antibody, FITC conjugated
    Biotin CSB-PA018565LD01HU PPP2R2B Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:500-1:1000
    IF 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity. Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance.
  • 基因功能参考文献：
    1. Dephosphorylation and total levels of beta-catenin were dependent on PR55beta in an adenoid cystic carcinoma tumor cell line. PMID: 29275362
    2. New pathogenic threshold of PPP2R2B CAG-43 to be pathogenic for SCA12 diagnosis. PMID: 27864267
    3. CAG triplet repeat expansion has been found in untranslated region of SCA12 gene in Indian spinocerebellar ataxia 12 patients. PMID: 27350687
    4. Thus, Fcp1 coordinates Cdk1 and Gwl inactivation to derepress PP2A-B55, generating a dephosphorylation switch that drives mitosis progression. PMID: 26653855
    5. investigated CAG copies within PPP2R2B in 29 patients with spinocerebellar ataxia who are excluded from the most common subtypes; 3 patients carried 53, 46 and 54 CAG repeats respectively, while the other 26 cases harbored CAG repeats less than 30 PMID: 25634432
    6. IER5 functions as a positive feedback regulator of HSF1 and that this process involves PP2A/B55 and HSF1 dephosphorylation. PMID: 25816751
    7. Bcl-2 phosphorylation at Ser70 by selective tyrosine nitration of PP2A-B56delta stabilizes its antiapoptotic activity PMID: 25082878
    8. Inactivation of protein-phosphatase 2A causing hyperphosphorylation of paraprotein targets in monoclonal gammopathy of undetermined significance, multiple myeloma and Waldenstrom's macroglobulinemia is due to an exchange of its regulatory subunits PMID: 24676687
    9. We demonstrate that PP2A/B55 is required for Gwl dephosphorylation at the essential Cdk site Thr194. Ensa/ARPP19 dephosphorylation is mediated by the RNA PMID: 24391510
    10. PP2A-B55beta promotes cyclin E1 overexpression by antagonizing its degradation and its inhibition could represent a therapeutic mechanism for abrogating cyclin E1 function in cancers PMID: 24509904
    11. Bod1 regulates protein phosphatase 2A at mitotic kinetochores. PMID: 24157919
    12. Differential autophagic neuronal cell death under stress is determined by the cytoplasmic and mitochondrial-specific PPP2R2B isoforms. PMID: 23381641
    13. Single nucleotide polymorphisms in PPP2R2B is associated with drug response in breast cancer. PMID: 23034890
    14. Data indicate that PPP2R2B gene product PP2A/Bbeta2 activates the mitochondrial fission enzyme dynamin-related protein 1 (Drp1) by dephosphorylating Ser656. PMID: 22583914
    15. This study suggested that PPP2R2B CAG expansion mutation might lead increase induction of Spinocerebellar ataxia type 12. PMID: 21827912
    16. defective production of PP2A Bbeta upon IL-2 deprivation results in apoptosis resistance and longer survival of autoreactive T cells, in a subset of SLE patients PMID: 21746932
    17. oxidative stress induced by mitochondrial dysfunction causes elevated expression of ppp2r2b and plays a causal role in SCA12; and reduction of ROS is a potential therapeutic intervention for this neuropathy PMID: 21471219
    18. results indicate that the A variant of the rs319217 SNP of the PPP2R2B gene is a marker of better prognosis in breast cancer PMID: 20669227
    19. CAG repeat lengths in the PPP2R2B gene may be potential genetic markers for Alzheimer disease susceptibility in the Japanese population. PMID: 21029765
    20. PPP2R2B, encoding the B55beta regulatory subunit of the PP2A complex, is epigenetically inactivated by DNA hypermethylation in colorectal cancer. PMID: 21075311
    21. Autosomal dominant cerebellar ataxia mapping to 5q31-q33.1 has no CAG repeat expansion or other mutations of the PPP2R2B gene. PMID: 20937954
    22. Quantitative methylation analysis identified ABCB1, FOXC1, PPP2R2B and PTEN as novel genes to be methylated in ductal carcinoma in situ. PMID: 20056007
    23. The CAG repeat in SCA12 functions as a cis element to up-regulate PPP2R2B expression. PMID: 20533062
    24. We investigated methylation patterns in the promoter regions of ABCB1, ATM, BRCA1, CDH3, CDKN2A, CXCR4, ESR1, FBXW7, FOXC1, GSTP1, IGF2, HMLH1, PPP2R2B, and PTEN75 in well-described pre-treatment samples from locally advanced breast cancer PMID: 20338046
    25. Cells with ectopically expressed PPP2R2B were shown predisposed to autophagy and oxidative stress induced cell death. PMID: 20017961
    26. a novel ATM-dependent mechanism is regulating association of B55 subunits with nuclear PP2A in response to IR PMID: 11723136
    27. Cyclin G recruits PP2A in order to modulate the phosphorylation of Mdm2 and thereby to regulate both Mdm2 and p53. PMID: 11983168
    28. Phosphorylation of bestrophin is regulated by PP2A. PMID: 12058047
    29. SCA12 triplet expansion rate in Italian ataxia patients PMID: 12140678
    30. Analysis of CTA/CTG and CAG polymorphism in a Polish control group. PMID: 14960773
    31. phospholipase D suppresses protein phosphatase 2A and is involved in the mTOR survival pathway in the transformation of human cells PMID: 16109716
    32. PP2A plays a positive rather than a negative role in the regulation of IKKbeta PMID: 16126728
    33. analysis of a founder for SCA12 in the Indian population PMID: 16138911
    34. We therefore propose that PP2A plays a crucial role in the maintenance of cell-cell adhesion through recruitment of IQGAP1 to the Rac-bound E-cadherin-catenins complex. PMID: 16245300
    35. The tumor suppressor PP2A is functionally inactivated in blast crisis CML through the inhibitory activity of the BCR/ABL-regulated SET protein. PMID: 16286244
    36. PP2A-mediated dephosphorylation of HSP27 and tau correlated with PP2A-induced preservation of endothelial cell cytoskeleton PMID: 16475161
    37. Cyclin G2 may modulate the cell cycle and cellular division processes through modulation of PP2A and centrosomal associated activities. PMID: 17123511
    38. The small t antigen (ST) of DNA tumor virus SV40 inhibits PP2A phosphatase activity through its N-terminal J domain. PMID: 17529992
    39. results suggest that the PPP2R2B gene CAG repeat polymorphism may be functional and may, in part, play a role in conferring susceptibility to Alzheimer disease and essential tremor in Taiwan PMID: 18484086
    40. Adenovirus E4orf4 protein downregulates MYC expression through interaction with the PP2A-B55 subunit PMID: 18653458
    41. Results suggest that Parkinsonism (PD) and multiple system atrophy (MSA) are not associated with spinocerebellar ataxia 12 (SCA12) and it is not necessary to screen SCA12 in PD and MSA patients. PMID: 18973067
    42. Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 18519826

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  • 相关疾病：
    Spinocerebellar ataxia 12 (SCA12)
  • 亚细胞定位：
    [Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Membrane.; [Isoform 2]: Cytoplasm. Mitochondrion. Mitochondrion outer membrane.
  • 蛋白家族：
    Phosphatase 2A regulatory subunit B family
  • 组织特异性：
    Brain.
  • 数据库链接：

    HGNC: 9305

    OMIM: 604325

    KEGG: hsa:5521

    STRING: 9606.ENSP00000336591

    UniGene: Hs.627618