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Phospho-ATRIP (Ser224) Antibody

  • 货号：
    CSB-PA832812
  • 规格：
    ￥2454
  • 图片：
    • Western blot analysis of extracts from 292 cells, treated with UV (15mins), using ATRIP (Phospho-Ser224) antibody. The lane on the right is treated with the synthesized peptide.
    • Western blot analysis of extracts from 293 cells (Lane 2) and Hela cells (Lane 3), using ATRIP (Phospho-Ser224) Antibody. The lane on the left is treated with synthesized peptide.
    • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ATRIP (Phospho-Ser224) antibody. The picture on the right is treated with the synthesized peptide.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ATRIP Polyclonal antibody
  • Uniprot No.：
    Q8WXE1
  • 基因名：
    ATRIP
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Peptide sequence around phosphorylation site of serine 224 (H-V-S(p)-P-R) derived from Human ATRIP.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
    IHC 1:50-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
  • 基因功能参考文献：
    1. ATRIP deacetylation by SIRT2 promotes ATR-ATRIP binding to replication protein A-single-stranded DNA to drive ATR activation and thus facilitate recovery from replication stress. PMID: 26854234
    2. ATRIP SUMOylation promotes ATR activation by providing a unique type of protein glue that boosts multiple protein interactions along the ATR pathway PMID: 24990965
    3. Data on crystal structure of BRCA1 binding with phosphopeptides suggest that C-terminal domain of BRCA1 interacts with ATRIP and BAAT1 with preferences for specific side chains; in ATRIP, phospho-Ser239 and Phe242 are the main interacting residues. PMID: 24073851
    4. Data indicate that ATRIP is a direct target gene of HIF-1, and the increased ATRIP then activates the ATR signaling pathway under hypoxia. PMID: 23454212
    5. our analysis exposes an overlapping clinical manifestation between the disorders but allows an expanded spectrum of clinical features for ATR-ATRIP Seckel Syndrome to be defined PMID: 23144622
    6. as an ATR-associated kinase, Nek1 enhances the stability and activity of ATR-ATRIP before DNA damage, priming ATR-ATRIP for a robust DNA damage response PMID: 23345434
    7. ATRIP may function outside the context of the canonical ATR damage signaling pathway during HSV-1 infection to participate in the viral life cycle. PMID: 20861269
    8. data suggest that RPA-coated ssDNA is the critical structure at sites of DNA damage that recruits the ATR-ATRIP complex and facilitates its recognition of substrates for phosphorylation and the initiation of checkpoint signaling PMID: 12791985
    9. there are at least two in vitro ATR-ATRIP DNA binding complexes, one which binds DNA with high affinity in an RPA-dependent manner and a second, which binds DNA with lower affinity in an RPA-independent manner PMID: 14724280
    10. Collectively, our results suggest that the ATR-mediated phosphorylation of ATRIP at Ser-68 and -72 is dispensable for the initial response to DNA damage. PMID: 15451423
    11. N-terminal domain of the ATRIP protein contributes to the cell cycle checkpoint by regulating the intranuclear localization of ATR PMID: 15527801
    12. ATRIP is required for ATR accumulation at intranuclear foci induced by DNA damage. PMID: 15743907
    13. ATRIP oligomerization is essential for the function of the ATM and Rad3-related (ATR)-ATRIP complex, which exists in higher order oligomeric states within cells PMID: 16027118
    14. The expression of dimerization-defective ATRIP diminishes the maintenance of replication forks during treatment with DNA replication inhibitors. PMID: 16176973
    15. support the idea of a multistep model for ATR activation that requires separable localization and activation functions of ATRIP PMID: 17339343
    16. a direct physical interaction between BRCA1 and ATRIP is required for the checkpoint function of ATR PMID: 17616665
    17. ATRIP is a CDK2 substrate, and CDK2-dependent phosphorylation of S224 regulates the ability of ATR-ATRIP to promote cell cycle arrest in response to DNA damage PMID: 17638878

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  • 亚细胞定位：
    Nucleus. Note=Redistributes to discrete nuclear foci upon DNA damage.
  • 蛋白家族：
    ATRIP family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 33499

    OMIM: 606605

    KEGG: hsa:84126

    STRING: 9606.ENSP00000323099

    UniGene: Hs.694840