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  4. Phospho-BLNK (Tyr84) Antibody

Phospho-BLNK (Tyr84) Antibody

  • 货号：
    CSB-PA246025
  • 规格：
    ￥2454
  • 图片：
    • Western blot analysis of extracts from K562 cells, treated with starved (24hours), using BLNK (Phospho-Tyr84) antibody. The lane on the right is treated with the synthesized peptide.
    • Immunohistochemistry analysis of paraffin-embedded human brain tissue using BLNK (Phospho-Tyr84) antibody. The picture on the right is treated with the synthesized peptide.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) BLNK Polyclonal antibody
  • Uniprot No.：
    Q8WV28
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Peptide sequence around phosphorylation site of tyrosine 84 (E-M-Y(p)-V-M) derived from Human BLNK.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.
  • 基因功能参考文献：
    1. vesicular signaling scaffolds are required for B cell activation indicates that vesicles may deliver preassembled signaling cargo to sites of BCR activation. PMID: 25140054
    2. early Ca(2+) fluxing provides feed-forward signal amplification by promoting anchoring of the PLCgamma2 C2 domain to phospho-SLP65. PMID: 24166973
    3. up-regulation of BLNK is associated with RUNX1 mutations in cytogenetically normal acute myeloid leukemia. PMID: 22689681
    4. Live cell imaging and co-immunoprecipitation experiments confirmed that both SLP65 and CIN85 are both required for the onset and progression phases of B-cell antigen receptor signal transduction. PMID: 21822214
    5. 16 of the 34 childhood pre-B acute lymphoblastic leukaemia samples that were tested showed a complete loss or drastic reduction of SLP-65 expression PMID: 12761551
    6. The BLNK protein is present in the majority of mediastinal B cell lymphomas. PMID: 15744341
    7. In B cells SLP-65 exists in a 180 kDa complex as well as in monomeric form. PMID: 16356554
    8. V(H) gene rearrangement represents a frequent feature in B-lymphoid malignancy, which can be attributed to SLP65 deficiency in many cases. PMID: 16636677
    9. Syk is required to link phosphorylated SLP-65 to Ca(2+) mobilization. PMID: 17681949
    10. Plasmacytoid dendritic cells express a signalosome consisting of Lyn, Syk, Btk, Slp65 (Blnk) and PLCgamma2. Triggering CD303 leads to tyrosine phosphorylation of Syk, Slp65, PLCgamma2 & cytoskeletal proteins. PMID: 18022864
    11. BLNK prevents aneuploidy by inhibiting cytokinesis. PMID: 19018766
    12. BLNK recruits active H-Ras to the BCR complex, which is essential for sustained surface expression of BCR in the form of the cap and for the signal leading to functional ERK activation PMID: 19218240
    13. Data show that SLP-65 phosphorylation acts upstream for signal initiation and also downstream during selective processing of the B cell receptor signal. PMID: 19372136
    14. Identifies BLNK as a potential target for the protein-tyrosine phosphatase SHP-1. PMID: 15588985

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  • 相关疾病：
    Agammaglobulinemia 4, autosomal recessive (AGM4)
  • 亚细胞定位：
    Cytoplasm. Cell membrane. Note=BCR activation results in the translocation to membrane fraction.
  • 组织特异性：
    Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
  • 数据库链接：

    HGNC: 14211

    OMIM: 604515

    KEGG: hsa:29760

    STRING: 9606.ENSP00000224337

    UniGene: Hs.665244