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  4. Phospho-Bad (Ser112) Antibody

Phospho-Bad (Ser112) Antibody

  • 货号：
    CSB-PA276323
  • 规格：
    ￥2454
  • 图片：
    • Western blot analysis of extracts from cos7 cells untreated or treated with EGF using BAD(Phospho-Ser112) Antibody
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Phospho-Ser112) Antibody(left) or the same antibody preincubated with blocking peptide(right).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Mus musculus (Mouse) Bad Polyclonal antibody
  • Uniprot No.：
    Q61337
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Peptide sequence around phosphorylation site of serine 112 (H-S-S(p)-Y-P) derived from Mouse BAD.
  • 免疫原种属：
    Mus musculus (Mouse)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
    IHC 1:50-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
  • 基因功能参考文献：
    1. This study shows for the first time that genetic knockout of Bad provides epileptic seizure protection in Kcna1-/- mice, a genetic model of epilepsy with sudden unexplained death. PMID: 29171006
    2. BAD knockout reduced epileptiform activity, and this effect was lost upon knockout or pharmacological inhibition of KATP channels. PMID: 29368690
    3. Bad is dispensable for TNF-mediated cell death. PMID: 25611386
    4. Results suggest that regulation of the proapoptotic activity of BAD plays a key role in the pathogenic mechanisms resulting in primary pigmented nodular adrenocortical disease tumor formation. PMID: 24865460
    5. fasting may increase the uptake beta-hydroxybutyrate by decreasing BAD in the brain during hypoglycemia PMID: 25043191
    6. Results indicate the downstream targets of insulin, cyclin D1, BAD, alpha-MHC, and GATA-4, elucidate a molecular mechanism of insulin in promoting cell proliferation and differentiation. PMID: 24020834
    7. our study suggests that Bad and Bmf co-regulate lymphocyte homeostasis and limit spontaneous transformation by mechanisms that may not exclusively be linked to the induction of lymphocyte apoptosis. PMID: 22430207
    8. Results reveal that IKK inhibits TNFalpha-induced apoptosis through two distinct but cooperative mechanisms: activation of the survival factor NF-kappaB and inactivation of the proapoptotic BH3-only BAD protein. PMID: 23332762
    9. RNAi-mediated silencing of STAT1 in soft tissue sarcoma (STS) cells was sufficient to increase expression of the apoptotic mediators Fas and Bad and to elevate the sensitivity of STS cells to Fas-mediated apoptosis PMID: 22805310
    10. BAD modulates counterregulatory responses to hypoglycemia and protective glucoprivic feeding PMID: 22162752
    11. the regulation of BAD by uremic toxins and report the sensitization of vascular smooth muscle cells to apoptosis upon BAD induction was explored. PMID: 22172950
    12. Tonicity-induced COX-2 expression and PGE2 synthesis in the renal medulla entails phosphorylation and inactivation of the pro-apoptotic protein Bad, thereby counteracting apoptosis in renal medullary epithelial cells. PMID: 21716255
    13. Caspase-3 is activated by the BAD-BAX cascade resulting in long term depression induction in the hippocampus. PMID: 21609830
    14. JNK1 is required for erythropoietin-mediated cell survival through phosphorylation and inactivation of the pro-apoptotic, Bcl-2 homology domain 3 (BH3)-only Bcl-associated death protein (Bad). PMID: 21095239
    15. Bad protein cooperate with bim protein in certain apoptotic responses and in the suppression of g-irradiation-induced thymic lymphoma.(Bad protein) PMID: 20431598
    16. Data show that loss of Bmf reduced the pressure to inactivate p53, whereas Bad deficiency did not, identifying Bmf as a novel component of the p53-independent tumor suppressor pathway triggered by c-Myc. PMID: 19965635
    17. The beta-arrestin 1-dependent ERK1/2 activation engaged by GLP-1 mediates the Ser-112 phosphorylation of Bad. PMID: 19915011
    18. The interaction of Bad with lipid rafts is a dynamic process regulated by IL-4 and involved in the control of apoptosis. PMID: 11907096
    19. activation by therapeutic inhibition of epidermal growth factor receptor and transactivation by insulin-like growth factor receptor PMID: 12011069
    20. Bcl-x(L) and Bcl-w target protein phosphatase 1alpha to Bad PMID: 12115603
    21. phosphorylation at serine 128 by activation of the JNK signaling pathway PMID: 12189144
    22. BAD phosphorylation protects cells from the deleterious effects of apoptotic stimuli and attenuates death pathway signaling by raising the threshold at which mitochondria release cytochrome c to induce cell death PMID: 12431371
    23. Bad apoptotic protein alone or in combination with bax apoptotic protein and the prostatic-specific promoter ARR(2)PB was an effective therapy for experimental prostatic neoplasms. PMID: 12490000
    24. Candida albicans phospholipomannan promotes survival of phagocytosed yeasts through modulation of of this protein's phosphorylation and macrophage apoptosis. PMID: 12551950
    25. HSV-1 US3 protein kinase blocks the caspases that cleave BAD at either residue 56 or 61 predicted to render the protein more proapoptotic or at residue 156, which would inactivate the protein. PMID: 12743316
    26. proapoptotic BAD suppresses tumorigenesis in the lymphocyte lineage PMID: 12876200
    27. combination of proteomics, genetics and physiology indicates an unanticipated role for BAD in integrating pathways of glucose metabolism and apoptosis PMID: 12931191
    28. PP2A dephosphorylation of pSer112 is the key initiating event regulating the activation of BAD during interleukin-3 withdrawal-induced apoptosis PMID: 12944463
    29. BAD is a substrate for pim-2 oncogene proto-oncogene PMID: 12954615
    30. regulation of Bad phosphorylation plays an active role in mediating anti-IgM-induced apoptosis of immature B cells PMID: 14585539
    31. JNK is required for IL-3-mediated cell survival through phosphorylation and inactivation of the proapoptotic Bcl-2 family protein BAD. PMID: 14967141
    32. Data show that the Bcl-2 homology 3 domain-only protein, Bad, is involved in cell death following IL-7 withdrawal from D1 cells, an IL-7-dependent murine thymocyte cell line. PMID: 15123689
    33. mechanisms that regulate the conversion of BAD from an anti-death to a pro-death factor include alternative splicing that produces N-terminally truncated BAD(S)and conversion by caspases into a pro-death fragment that resembles the short splice variant PMID: 15231831
    34. alteration of lipid rafts is an early event in the apoptotic cascade indirectly induced by interleukin-4 deprivation via PP1alpha activation, dephosphorylation of cytoplasmic Bad, and caspase activation PMID: 15634756
    35. Bad phosphorylation is not essential for PKB-mediated survival signaling in hemopoietic cells PMID: 15843895
    36. Pak1-dependent Raf-1 phosphorylation regulates its mitochondrial localization, phosphorylation of BAD, and Bcl-2 association PMID: 15849194
    37. BAD induces apoptosis upon detecting the coincidence of G2/M phase and growth factor deprivation PMID: 15901741
    38. phosphorylation of BAD Serine 128 exerts cell-specific effects on apoptosis PMID: 15907327
    39. All three Pim kinase family members predominantly phosphorylate Bad on Ser112 and in addition are capable of phosphorylating Bad on multiple sites associated with the inhibition of the pro-apoptotic function of Bad in HEK-293 cells PMID: 16403219
    40. cellular cholesterol biosynthesis is critical for the activation and maintenance of the Akt-Bad cell survival cascade in response to growth factors such as insulin. PMID: 16513830
    41. These data establish a connection between calcium overload and mitochondria-mediated death pathways in outer hair cells and also suggest a dual role for BAD. PMID: 16521126
    42. the interaction of BAD with membranes is tied to binding of 14-3-3 protein and activation and membrane translocation of Bcl-XL PMID: 16603546
    43. Study shows, using spectroscopic methods, that the BH3-only proteins Bim, Bad and Bmf are unstructured in the absence of binding partners. PMID: 16645638
    44. Bad was not required for cell death following IL-3 withdrawal, suggesting changes to phosphorylation of Bad play only a minor role in apoptosis. PMID: 16705087
    45. Both gonadotropin releasing hormone andepidermal growth factor (EGF) caused rapid phosphorylation of BAD. PMID: 16741954
    46. The proapoptotic protein Bad is a key player in cell survival decisions, and is regulated post-translationally by several signaling networks. PMID: 17535812
    47. Raf-1 in beta-cells led to a striking loss of Bad phosphorylation at serine 112 and an increase in the protein levels of both Bad and Bax PMID: 18006502
    48. These findings provide genetic proof of the bifunctional activities of BAD in both beta cell survival and insulin secretion. PMID: 18223655
    49. Thr-201 phosphorylation of Bad by JNK1 is required for PFK-1 activation PMID: 18469002
    50. BAD is the only BCL-2 family protein expressed in parietal cells PMID: 18779780

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  • 亚细胞定位：
    Mitochondrion outer membrane. Cytoplasm.
  • 蛋白家族：
    Bcl-2 family
  • 数据库链接：

    KEGG: mmu:12015

    STRING: 10090.ENSMUSP00000025910

    UniGene: Mm.4387