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  4. Phospho-HGS (Y216) Antibody

Phospho-HGS (Y216) Antibody

  • 货号：
    CSB-PA000543
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of A549 cells using Phospho-Hrs (Y216) Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    O14964
  • 基因名：
  • 别名：
    Hepatocyte growth factor regulated tyrosine kinase substrate antibody; Hepatocyte growth factor-regulated tyrosine kinase substrate antibody; HGF-regulated tyrosine kinase substrate antibody; HGNC:4897 antibody; HGS antibody; HGS_HUMAN antibody; Hrs antibody; Human growth factor regulated tyrosine kinase substrate antibody; Protein pp110 antibody; ZFYVE8 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from Human Hrs around the phosphorylation site of Y216.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    ELISA 1:10000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
  • 基因功能参考文献：
    1. Results demonstrate the existence of crosstalk between beta-catenin signaling and HGS in two different types of cancer (hepatoblastoma and colorectal). PMID: 26715116
    2. detailed analysis of the subcellular localization and functional significance of Hrs in macropinocytosis-mediated entry of Kaposi's Sarcoma-Associated Herpesvirus PMID: 26819309
    3. we identified an interaction between EsxH, which is secreted by the Esx-3 TSSS, and human hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs/Hrs), a component of the endosomal sorting complex required for transport (ESCRT). PMID: 24204276
    4. the role for tumoral c-Met expression or sMet/HGF levels as upfront selection criterion or predictive biomarkers deserve further study in this emerging area of therapeutic focus in RCC PMID: 23867513
    5. Hrs is a regulator of endosomal cholesterol trafficking. PMID: 22832105
    6. Hrs tyrosine phosphorylation detected upon EGF-stimulation. PMID: 22800866
    7. These results suggested that HCV secretion from host cells requires Hrs-dependent exosomal pathway in which the viral assembly is also involved. PMID: 22138215
    8. Hrs inhibits HIV-1 production by inhibiting citron kinase-mediated exocytosis. PMID: 21748597
    9. ESCRT-0 component HRS is required for HIV-1 Vpu-mediated BST-2/tetherin down-regulation. PMID: 21304933
    10. Plasma hepatocyte growth factor is associated with periampullary cancer. PMID: 20812348
    11. hSpry2 binds to the endocytic regulatory protein, hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) and blocks intracellular signal propagation. PMID: 17320394
    12. The HRS domain required for merlin binding is narrowed to a region (residues 470-497) containing the predicted coiled-coil domain whereas the major domain responsible for HRS growth suppression is distinct (residues 498-550). PMID: 12444101
    13. HRS inhibits Stat3 activation in schwannoma cells. PMID: 12444102
    14. Overexpression Hgs in T cells yielded a dose-dependent decrease in cotransfected reporter gene expression, indicating an inhibitory function of this molecule. PMID: 12554698
    15. Data suggested that association with Hrs is a prerequisite for signal transducing adaptor molecule function. PMID: 15113837
    16. Hrs regulates the sorting of ligand-stimulated and unstimulated growth factor receptors on early endosomes, and the FYVE domain, which is required for Hrs to reside in a microdomain of early endosomes. PMID: 15212941
    17. the endosome-associated protein hrs is a subunit of a protein complex containing actinin-4, BERP, and myosin V that is necessary for efficient TfR recycling but not for EGFR degradation PMID: 15772161
    18. Hrs plays role in a cargo-specific recycling mechanism, which is critical to controlling functional activity of the largest known family of signaling receptors. PMID: 15944737
    19. role of HRS in up-regulating MAPK, presumably involving interaction with PELP1. PMID: 16352611
    20. Hgs is a novel Smad5 interactor and an inhibitor of bone morphogenetic protein (BMP) signaling. PMID: 16516194
    21. These results indicate that Tsg101 is required for the formation of stable vacuolar domains within the early endosome that develop into multivesicular body (MVBs) and Hrs is required for the accumulation of internal vesicles within MVBs. PMID: 16707569
    22. HGS and GUK1 were significantly over expressed in GH-secreting adenomas, compared with ACTH-secreting adenomas and nonfunctioning tumors, and with PRL-secreting adenomas, respectively. PMID: 16832584
    23. Four proteins (TSG101,Hrs,Aip1/Alix, and Vps4B) of the ESCRT (endosomal sorting complex required for transport) machinery were localized in T cells and macrophages by quantitative electron microscopy. PMID: 17014699
    24. Specific sequence in the beta2-adrenergic receptor cytoplasmic tail confers Hrs dependence on receptor recycling. PMID: 17138565
    25. We conclude that Hrs is a positive regulator of VEGF-R2 and IR signaling and that ectopic expression of Hrs protects both VEGF-R2 and IR from degradation. PMID: 17445799
    26. Targeted disruption of Hrs by small interfering RNA effectively attenuated the proliferation, anchorage-independent growth, tumorigenesis, and metastatic potential of HeLa cells in vitro and in vivo. PMID: 17545595
    27. propose a novel function of Hrs, as a crucial player in the maturation of autophagosomes PMID: 17624298
    28. HRS mediates post-endocytic trafficking of protease-activated receptor 2 and calcitonin receptor-like receptor PMID: 17675298
    29. PELP1 and HRS reallocate to autophagosomes in response to resveratrol treatment, which might be important in the process of autophagy in the cancer cells. PMID: 17804729
    30. These results indicate that Eps15b is an endosomally localized isoform of Eps15 that is present in the Hrs complex via direct Hrs interaction and important for the sorting function of this complex. PMID: 18362181
    31. these results indicate that precise sorting of IL-2Rbeta from early to late endosomes is mediated by Hrs, a known sorting component of the ubiquitin-dependent machinery, in a manner that is independent of UIM-ubiquitin binding PMID: 18445679
    32. Data show that Trak1 interacts with hepatocyte-growth-factor-regulated tyrosine kinase substrate (Hrs), an essential component of the endosomal sorting and trafficking machinery. PMID: 18675823

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  • 亚细胞定位：
    Cytoplasm. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Endosome, multivesicular body membrane; Peripheral membrane protein.
  • 组织特异性：
    Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
  • 数据库链接：

    HGNC: 4897

    OMIM: 604375

    KEGG: hsa:9146

    STRING: 9606.ENSP00000331201

    UniGene: Hs.661056