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  4. Phospho-HIRA (T555) Antibody

Phospho-HIRA (T555) Antibody

  • 货号：
    CSB-PA060029
  • 规格：
    ￥880
  • 图片：
    • Immunohistochemistry of paraffin-embedded human brain using CSB-PA060029 at dilution of 1:100
    • Immunofluorescent analysis of Hela cells using CSB-PA060029 at dilution of 1:200 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 其他：

产品详情

  • Uniprot No.：
    P54198
  • 基因名：
    HIRA
  • 别名：
    DGCR1 antibody; DiGeorge critical region gene 1 antibody; HIR antibody; HIR histone cell cycle regulation defective homolog A antibody; HIRA antibody; HIRA protein antibody; HIRA_HUMAN antibody; Histone cell cycle regulator antibody; Protein HIRA antibody; TUP 1 antibody; TUP1 like enhancer of split protein 1 antibody; TUP1-like enhancer of split protein 1 antibody; TUPLE1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from Human HIRA around the phosphorylation site of T555.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    IHC, IF, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:100-1:300
    IF 1:200-1:1000
    ELISA 1:10000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
  • 基因功能参考文献：
    1. These data show that HIRA phosphorylation limits the expression of myogenic genes, while the dephosphorylation of HIRA is required for proficient H3.3 deposition and gene activation, demonstrating that the phosphorylation switch is exploited to modulate HIRA/H3.3-mediated muscle gene regulation during myogenesis. PMID: 27515126
    2. Data show that histone chaperone HIRA co-localizes with viral genomes, binds to incoming viral and deposits histone H3.3 onto these. PMID: 28981850
    3. Chromatin reassembly during double-strand break repair was dependent on the HIRA histone chaperone that is specific to the replication-independent histone variant H3.3 and on CAF-1 that is specific to the replication-dependent canonical histones H3.1/H3.2. PMID: 27269284
    4. RPA, best known for its role in DNA replication and repair, recruits HIRA to promoters and enhancers and regulates deposition of newly synthesized H3.3 to these regulatory elements for gene regulation. PMID: 28107649
    5. H3.Y discriminates between HIRA and DAXX chaperone complexes and reveals unexpected insights into human DAXX-H3.3-H4 binding and deposition requirements. PMID: 28334823
    6. PHB has an unexpected nuclear role in human embryonic stem cells that is required for self-renewal and that it acts with HIRA in chromatin organization to link epigenetic organization to a metabolic circuit. PMID: 27939217
    7. The abnormal lower expression of the HIRA gene in the myocardium may participate in the pathogenesis of Tetralogy of Fallot. PMID: 27748330
    8. These results support a model in which OGT modifies HIRA to regulate HIRA-H3.3 complex formation and H3.3 nucleosome assembly and reveal the mechanism by which OGT functions in cellular senescence. PMID: 27217568
    9. HIRA controls a specialized, dynamic H4K16ac-decorated chromatin landscape in senescent cells and enforces tumor suppression. PMID: 25512559
    10. Mechanistic studies reveal that HIRA accumulates at sites of UVC irradiation upon detection of DNA damage prior to repair and deposits newly synthesized H3.3 histones. This local action of HIRA depends on ubiquitylation events associated with damage recognition. PMID: 24074863
    11. HIRA is required for deposition of histone H3.3 at its binding sites. PMID: 23602572
    12. NHRD domain of UBN1 as being an essential region for HIRA interaction and chromatin organization by the HUCA complex PMID: 22401310
    13. HIRA plays a unique, ASF1a-independent role, which is required for the localization of HP1 PMID: 21347226
    14. Data show that, like HIRA, UBN1, and ASF1a, CABIN1 is involved in heterochromatinization of the genome of senescent human cells. PMID: 21807893
    15. phosphorylation of histone H4 Ser 47 catalyzed by the PAK2 kinase, promotes nucleosome assembly of H3.3-H4 and inhibits nucleosome assembly of H3.1-H4 by increasing the binding affinity of HIRA to H3.3-H4 and reducing association of CAF-1 with H3.1-H4 PMID: 21724829
    16. the N-terminal half of HIRA should contribute positively to the growth rate via up-regulation of a set of cell cycle-related genes, whereas the C-terminal half down-regulated another set of them without exhibiting any effect on the cell growth PMID: 16024922
    17. The N- and C-terminal regions of ASF1a and ASF1b determine the different affinities of these two proteins for HIRA, by contacting regions outside the HIRA B domain. CAF-1 also uses B domain-like motifs for binding to ASF1a, thereby competing with HIRA. PMID: 16980972
    18. Hpc2-related domain of UBN1, UBN2, and Hpc2p is an evolutionarily conserved HIRA/Hir-binding domain, which directly interacts with the N-terminal WD repeats of HIRA/Hir. PMID: 19029251
    19. FISH analysis of metaphases revealed a duplication of TUPLE1 probe on one chromosome 22q (Fig. 1). PMID: 19046189

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  • 亚细胞定位：
    Nucleus. Nucleus, PML body. Note=Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.
  • 蛋白家族：
    WD repeat HIR1 family
  • 组织特异性：
    Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.
  • 数据库链接：

    HGNC: 4916

    OMIM: 600237

    KEGG: hsa:7290

    STRING: 9606.ENSP00000263208

    UniGene: Hs.474206