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  4. Phospho-HNRNPK (Ser216) Antibody

Phospho-HNRNPK (Ser216) Antibody

  • 货号：
    CSB-PA049174
  • 规格：
    ￥2454
  • 图片：
    • Western blot analysis of extracts from JurKat cells, using hnRNP K (Phospho-Ser216) antibody. The lane on the right is treated with the synthesized peptide.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) HNRNPK Polyclonal antibody
  • Uniprot No.：
    P61978
  • 基因名：
    HNRNPK
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Peptide sequence around phosphorylation site of serine 216 (S-E-S(p)-P-I) derived from Human hnRNP K.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.
  • 基因功能参考文献：
    1. Data show that heterogeneous nuclear ribonucleoprotein K (hnRNP K) and influenza virus NS1A binding protein (NS1-BP) regulate host splicing events and that viral infection causes mis-splicing of some of these transcripts. PMID: 29921878
    2. Study findings underscore the biological significance of MRPL33-L and hnRNPK in the tumor formation and identifies hnRNPK as a critical splicing regulator of MRPL33 pre-mRNA in cancer cells. PMID: 28869607
    3. hnRNPK regulates PLK1 expression by competing with the PLK1-targeting miRNAs, miR-149-3p and miR-193b-5p. PMID: 28708135
    4. we discovered that hnRNPK plays an important role in bladder cancer, suggesting that it is a potential prognostic marker and a promising target for treating bladder cancer. PMID: 27862976
    5. The hnRNPK positively regulates the level of prostate tumor overexpressed 1-antisense 1 which harbors five binding sites for miR-1207-5p. The knockdown of hnRNPK or PTOV1-AS1 increased the enrichment of heme oxygenase-1 mRNA in miR-1207-5p-mediated miRNA-induced silencing complex and thus suppressed the expression of heme oxygenase-1. PMID: 28228215
    6. High HNRNPK expression is associated with pancreatic cancer. PMID: 28423622
    7. Down-regulation of DAB2IP correlated negatively with hnRNPK and MMP2 expressions in CRC tissues. In conclusion, our study elucidates a novel mechanism of the DAB2IP/hnRNPK/MMP2 axis in the regulation of CRC invasion and metastasis, which may be a potential therapeutic target. PMID: 28335083
    8. Tumor cells bearing a p53 mutation showed increased damage levels and delayed repair. Knockdown of hnRNPK applied simultaneously with irradiation reduced colony-forming ability and survival of tumor cells. Taken together, our data shows that hnRNPK is a relevant modifier of DNA damage repair and tumor cell survival. We therefore recommend further studies to evaluate the potential of hnRNPK as a drug target for improvement PMID: 28426877
    9. Data suggest that hnRNPK plays role in heat shock response of cells by regulating HSF1; hnRNPK inhibits HSF1 activity, resulting in reduced expression of HSP27 and HSP70 mRNAs; hnRNPK also down-regulates binding of HSF1 to heat shock response element. (hnRNPK = heterogeneous-nuclear ribonucleoprotein K; HSF1 = heat shock transcription factor 1; HSP = heat-shock protein) PMID: 28592492
    10. KRAS-mutant colorectal carcinoma shows intrinsic radioresistance along with rapid upregulation of hnRNP K in response to ionizing radiation that can effectively be targeted by MEK inhibition. PMID: 27793696
    11. Nujiangexathone A, a novel compound from Garcinia nujiangensis, down-regulates hnRNPK levels in cervical tumor cells, inducing cell cycle arrest. PMID: 27424288
    12. Our study also suggests that loss of function variants in HNRNPK should be considered as a molecular basis for patients with Kabuki-like syndrome PMID: 26954065
    13. these findings support a critical role for hnRNP K in the regulation of autophagy in drug-resistant leukemia cells, and this RNP is therefore a potential target of clinical drug-resistance treatment. PMID: 27155326
    14. hnRNP K is a multifunctional protein that can regulate both oncogenic and tumor suppressive pathways through a plethora of chromatin-, DNA-, RNA-, and protein-mediated activates, suggesting its aberrant expression may have broad-reaching cellular impacts. (Review) PMID: 27049467
    15. These results establish the role of hnRNP K and PCPB1 in the translational control of morphine-induced MOR expression in human neuroblastoma (NMB) cells as well as cells stably expressing MOR (NMB1). PMID: 27292014
    16. HnRNP K is a promising tissue biomarker for diagnosing gastric cancer. PMID: 27278897
    17. CASC11 can target heterogeneous ribonucleoprotein K (hnRNP-K) to activate WNT/beta-catenin signaling in colorectal cancer cells to promote tumor growth and metastasis. PMID: 27012187
    18. Data show that heterogeneous nuclear ribonucleoprotein K (hnRNPK) stabilized of cellular FLICE-inhibitory protein (c-FLIP) protein through inhibition of glycogen synthase kinase 3 beta (GSK3beta) Ser9 phosphorylation during the TNF-related aoptosis-inducing ligand (TRAIL)-induced apoptosis. PMID: 26972480
    19. HhnRNP-K-mediated regulation of NMHC IIA mRNA translation contributes to the control of enucleation in erythropoiesis. PMID: 26823606
    20. hnRNP K binds miR-122, a mature liver-specific microRNA required for Hepatitis C virus replication. PMID: 26330540
    21. The s identified two new human proteins that interact with Ehrlichia chaffeensis EtpE-C: CD147 and heterogeneous nuclear ribonucleoprotein K (hnRNP-K). PMID: 26530384
    22. Data show that proto-oncogene protein c-myc is upregulated by sumoylated heterogeneous nuclear ribonucleoprotein K (hnRNP K) at the translational level in Burkitt's lymphoma cells. PMID: 26317903
    23. hnRNP K may be a key molecule involved in cell motility in RCC cells PMID: 26713736
    24. It describes the identification of heterogeneous nuclear ribonucleoprotein K (hnRNPK) as one of the composite element binding factors(CEBF) that acts as transactivator of PXR promoter. PMID: 26586566
    25. RTVP-1 regulates glioma cell spreading, migration and invasion and that these effects are mediated via interaction with N-WASP and by interfering with the inhibitory effect of hnRNPK on the function of this protein. PMID: 26305187
    26. we investigate the role of hnRNP K in the radioresistance of malignant melanoma cells PMID: 26136337
    27. These results indicate that dengue virus type 2 and Junin virus induce hnRNP K cytoplasmic translocation to favor viral multiplication. PMID: 25865411
    28. Data implicate hnRNPK in the development of hematological disorders and suggest hnRNPK acts as a tumor suppressor. PMID: 26412324
    29. Inhibition of CDK2 phosphorylation blocked phosphorylation of hnRNP K, preventing its incorporation into stress granules (SGs). Due to interaction between hnRNP K with TDP-43, the loss of hnRNP K from SGs prevented accumulation of TDP-43. PMID: 25410660
    30. HNRNPK might determine efficiency of Hepatitis C virus particle production by limiting the availability of viral RNA for incorporation into virions. PMID: 25569684
    31. Data indicate that twenty proteins were identified as binding partners of the primary activating element in the heterogeneous nuclear ribonucleoprotein K (hnRNP K) promoter. PMID: 25497182
    32. hnRNP K interacts with EV71 5' UTR, which is required for efficient synthesis of viral RNA.[review] PMID: 26164948
    33. hnRNPK is potentially implicated in the radiogenic response of HNSCC. PMID: 25281771
    34. hnRNP K plays an important role in the mitotic process in colon cancer cells.hnRNP K upregulates NUF2 and promotes the tumorigenicity of colon cancer cells. PMID: 25701787
    35. These findings functionally integrate K17, hnRNP K, and gene expression along with RSK and CXCR3 signaling in a keratinocyte-autonomous axis and provide a potential basis for their implication in tumorigenesis PMID: 25713416
    36. HnRNP K can induce MMP12 expression and enzyme activity through activating MMP12 promoter, which promotes cell migration and invasion in nasopharyngeal carcinoma cells. PMID: 24885469
    37. Results indicate that the interaction between the AR and hnRNP K has an important role in the progression of prostate cancer. PMID: 24626777
    38. hnRNP K and PU.1 act synergistically during granulocytic differentiation, hnRNP K seems to have a negative effect on PU.1 activity during monocytic maturation PMID: 25005557
    39. CNBP overexpression caused increase of cell death and suppression of cell metastasis through its induction of G-quadruplex formation in the promoter of hnRNP K resulting in hnRNP K down-regulation PMID: 24594223
    40. Data show that SET accumulation up-regulated hnRNPK mRNA and total/phosphorylated protein, promoted hnRNPK nuclear location, and reduced Bcl-x mRNA levels. PMID: 24508256
    41. NS1-BP-hnRNPK complex is a key mediator of influenza A virus gene expression. PMID: 23825951
    42. These studies demonstrate hnRNP K to be a multifunctional protein that supports vesicular stomatitis virus infection via its role(s) in suppressing apoptosis of infected cells. PMID: 23843646
    43. hnRNPK may play a role in recruitment of XRN2 to gene loci thus regulating coupling 3'-end pre-mRNA processing to transcription termination. PMID: 23857582
    44. heterogeneous nuclear ribonucleoprotein K (hnRNPK), a protein known to integrate multiple signal transduction pathways with gene expression, as a SERT distal polyadenylation element binding protein PMID: 23798440
    45. Prolonged downregulation of hnRNP K using small interfering RNA significantly decreased cell viability and increased apoptosis in HCC cell lines in a p53-independent manner. PMID: 23455382
    46. Caspase-3 cleaves hnRNP K in erythroid differentiation. PMID: 23519117
    47. HnRNP K controlled expression of IE2 protein of Human cytomegalovirus during viral replication. PMID: 23099853
    48. hnRNP-K regulates extracellular matrix, cell motility, and angiogenesis pathways. Involvement of the selected genes (Cck, Mmp-3, Ptgs2, and Ctgf) and pathways was validated by gene-specific expression analysis PMID: 23564449
    49. ATM-dependent phosphorylation of heterogeneous nuclear ribonucleoprotein K promotes p53 transcriptional activation in response to DNA damage. PMID: 23343766
    50. SUMO modification plays a crucial role in the control of hnRNP-K's function as a p53 co-activator in response to DNA damage by UV PMID: 23092970

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  • 相关疾病：
    Au-Kline syndrome (AUKS)
  • 亚细胞定位：
    Cytoplasm. Nucleus, nucleoplasm. Cell projection, podosome. Note=Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036). In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702).
  • 数据库链接：

    HGNC: 5044

    OMIM: 600712

    KEGG: hsa:3190

    STRING: 9606.ENSP00000365439

    UniGene: Hs.522257