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  4. Phospho-HSPB1 (Ser15) Antibody

Phospho-HSPB1 (Ser15) Antibody

  • 货号：
    CSB-PA072898
  • 规格：
    ￥2454
  • 图片：
    • Western blot analysis of extracts from Hela cells untreated or treated with UV using HSP27(Phospho-Ser15) Antibody.
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27(Phospho-Ser15) Antibody(left) or the same antibody preincubated with blocking peptide(right).
    • Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using HSP27(Phospho-Ser15) Antibody.
    • Western blot analysis of extracts from Hela cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using HSP27 (Phospho-Ser15) Antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) HSPB1 Polyclonal antibody
  • Uniprot No.：
    P04792
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Peptide sequence around phosphorylation site of serine15 (G-P-S(p)-W-D) derived from Human HSP27.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC,IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
    IHC 1:50-1:100
    IF 1:100-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.
  • 基因功能参考文献：
    1. Data suggest that both the small heat shock protein HspB1/Hsp27 and the constitutive chaperone Hsc70/HspA8 interact with tau to prevent tau-fibril/amyloid formation. HspB1 delays tau-fibril formation by weakly interacting with early species in aggregation process. (HspB1 = heat shock protein family B small member 1; Hsc70 = heat shock protein family A Hsp70) PMID: 29298892
    2. HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis. PMID: 29650953
    3. Results show that HSP27 expression is significantly increased in metastatic prostate cancer and represents a strong marker predicting survival, along with ALDH6A1 and prohibitin. PMID: 30396985
    4. High HSP27 expression is associated with breast cancer. PMID: 30106436
    5. our data suggest that overexpression of peroxiredoxin-2, annexin A2, and heat shock protein beta-1 was correlated with tumor invasion, metastasis, and poor prognosis, and therefore, these proteins may serve as potential diagnostic and therapeutic biomarkers. PMID: 29332450
    6. ETAS down-regulated HSP27 and pHSP27 (serine 78) in KLM1-R cells, but, HSP70 and GRP78 levels were not altered. This study suggests the potential therapeutic benefit of ETAS in enhancing anticancer effects by its combination with gemcitabine for patients with pancreatic cancer PMID: 29936456
    7. Silencing of HSP27 increases curcumin resistance by reducing apoptosis and reactive oxidative stress production in colon cancer. PMID: 30056019
    8. The expression of type I and III collagen in the TGF-beta1induced transition of A549 cells to myofibroblasts was significantly downregulated by transfection of A549 cells with HSP27 plasmid Dinterfering sequence and optimal ratio of Lipofectamine(R) 2000 and HSP27 plasmid. PMID: 29568951
    9. It was suggested that the HSP27 expression in EUS-FNA samples was useful for predicting GEM sensitivity. PMID: 29695571
    10. High hsp27 expression is associated with leukemia. PMID: 29328466
    11. p-Hsp27 is a novel biomarker of glioma and might have important clinical value for further classification of patients with wild-type IDH1 and normal ATRX expression and for evaluating prognosis. PMID: 29550762
    12. Dimer-monomer equilibrium of human HSP27 is influenced by the in-cell macromolecular crowding environment and is controlled by fatty acids and heat. PMID: 29635040
    13. HSP27 expression promotes vascular smooth muscle cell viability, suppresses cell apoptosis, and confers protection against oxidative stress in Thoracic aortic dissection. PMID: 29209372
    14. Hsp27 and P38MAPK could be used as prognostic factors in Esophageal squamous cell carcinoma. PMID: 29099815
    15. Addition of apatorsen to chemotherapy does not improve outcomes in unselected patients with metastatic pancreatic cancer in the first-line setting, although a trend toward prolonged PFS and OS in patients with high baseline serum Hsp27 suggests this therapy may warrant further evaluation in this subgroup PMID: 28935773
    16. High HSP27 expression is associated with non-small cell lung cancer metastasis. PMID: 28656293
    17. Knockdown of HSP27 blocked TGF-beta-induced cisplatin resistance. PMID: 28848138
    18. The expression of HSP27 was approximately 2-fold higher in apical periodontitis. Next, an increased expression of HSP27 was detected in epithelial cells PMID: 28673495
    19. Findings indicate the heat-shock protein 27 (Hsp27) pathway as a therapeutic target for the management of conditions featuring dysregulated interleukin 1 beta (IL-1beta) production. PMID: 27976724
    20. Increased protein level of HSP27 through SUMO2/3-mediated SUMOylation plays crucial roles in the progression of primary hepatocellular carcinoma. PMID: 28665748
    21. When the correlations of the markers with the response to neoadjuvant chemotherapy were examined, only high pre-chemotherapy levels of cytoplasmic HSPB1/p correlated with a poor clinical and pathological response to neoadjuvant cisplatin chemotherapy (p = 0.056) suggesting that this marker could be useful opening its study in a larger number of cases. PMID: 28608263
    22. Exposure to cetuximab and various concentration of AG490, an inhibitor of JAK2, STAT3 and HSP27 protein levels, except in the KRAS G12V mutant line, SW620...cetuximab may promote SN38 sensitivity via suppression of HSP27, through blocking the JAK/STAT signaling pathway, and shows synergistic effects when combined with SN38 in wild-type RAS CRC cells. PMID: 28656305
    23. we concluded that HSP27-silenced placenta-derived multipotent cells differentiated into neurons possessing the characteristics of functional glutamatergic neurons. PMID: 27444754
    24. Data show that Hsp27increases degradation rate of ubiquitinated MST1 and therefore interrupts the Hippo pathway kinase cascade. Consequently YAP and TAZ are less phosphorylated, free to translocate into the nucleus promoting a malignant phenotype. These findings underscore the central importance of Hsp27 in regulating multiple signaling pathways that promote tumor aggressiveness. PMID: 27555231
    25. upregulation of Hsp27 is a common phenomenon shared between pregnancies in patients with preterm prelabor rupture of membranes and spontaneous preterm labor with intact membranes PMID: 28497897
    26. HSP27 is an independent predictor of prognosis in chronic HF PMID: 28844461
    27. Hsp27 may up-regulate the expression of ABCA1 and promotes cholesterol efflux through activation of the PI3K/PKCzeta/Sp1 signal pathway in THP-1 macrophage-derived foam cells PMID: 28610841
    28. This study reports solution-state nuclear magnetic resonance spectroscopy investigations of the conformation and dynamics of the disordered and flexible C-terminal region of human HSP27. These data indicate a potential role for cis-trans proline isomerization in regulating the oligomerization. PMID: 28547731
    29. Overexpression of both HSPB5 and Hsp27 significantly reduced the intracellular aggregation of alpha-synuclein. PMID: 28337642
    30. HspB1 structural organization displays dynamic and complex rearrangements in response to changes in the cellular environment or when the cell physiology is modified. [review] PMID: 28144778
    31. Glutathione-S-transferase - HspB1 fusion protein prevents more aggregation of malate dehydrogenase compared to glutathione-S-transferase -HspB5 fusion protein and wild type HspB1. PMID: 28130664
    32. The data suggest that oncogene-addicted cells require the small heat-shock protein of 27 kDa (HSP27) for survival and that HSP27 might interfere with the effectiveness of targeted agents. PMID: 28182330
    33. Data indocate six cytostatic drugs which inhibit heat shock 27 kDa protein (HSP27) and tackle drug resistance by computational drug repositioning approach. PMID: 27626687
    34. Different from C-M-T phenotype in hereditary neuropathies caused by mutations in the HSPB1 gene. PMID: 27816334
    35. found knock down of HSPB1 further increased the proportion of apoptotic cells in hyperthermic treated melanoma cells when compared with either single agent alone, and both agents leaded to cell cycle arrest at G0/G1 or G2/M phases PMID: 27626679
    36. Data suggest that altered dimerization of HSP27 represents a good strategy for anticancer therapy in HSP27-overexpressing cancer cells. PMID: 27449291
    37. Apatorsen ( 2'-methoxyethyl-modified antisense oligonucleotide that inhibits Hsp27 expression)was tolerated at the highest dose evaluated (1000 mg). Single-agent activity was suggested by changes in tumor markers, circulating tumor cells , and stable measurable disease PMID: 27022067
    38. this results show the central role of Hsp27 in RACK1 pseudosubstrate or LPS-induced cell activation of primary leukocytes PMID: 27178349
    39. High p-Hsp27 expression is associated with cisplatin resistance in lung cancer. PMID: 26872057
    40. Low p-Hsp27 expression is associated with pancreatic cancer. PMID: 26895107
    41. The up-regulation of Hsp27 by E2 is mediated by ERalpha/Sp1. PMID: 27179757
    42. Study report a novel interaction between mutant HSPB1-P182L and the RNA binding protein PCBP1, leading to a reduction in its translational repression activity. Identifying PCBP1 mRNA targets revealed a marked prevalence for an RNA recognition motif, preferably seen in their 5' and 3'UTRs. Findings further support a role for mutant HSPB1 in neurodegenerative diseases. PMID: 28077174
    43. Hsp 70 and Hsp 27 were expressed in middle ear effusions PMID: 28964286
    44. High Hsp27 expression is associated with Thyroid Tumors. PMID: 26970173
    45. Bradykinin stimulates myofibroblast migration through protein kinase D-mediated activation of COX-2 and Hsp27. PMID: 28032559
    46. Data suggest that HSPB6 forms hetero-oligomers with HSPB1 under the following rules: (1) highly conserved motif RLFDQXFG is necessary for subunit exchange among oligomers, (2) a site about 20 residues downstream of this motif determines size of resultant hetero-oligomers, and (3) a region in the N-terminal domain that is unique to HSPB6 dictates preferential formation of heterodimers. (HSP = heat shock protein) PMID: 28487364
    47. TNF-alpha stimulates colonic myofibroblast migration through P38 MAPK-mediated activation of COX-2 and Hsp27. PMID: 27451881
    48. The potency of the sHsps to suppress aggregation of model substrates is correlated with their ability to form stable substrate complexes; especially HspB1 and HspB5, but also B3, bind tightly to a variety of proteins PMID: 27909051
    49. This work examines the molecular mechanism by which two canonical sHsps, alphaB-crystallin (alphaB-c) and Hsp27, interact with aggregation-prone alpha-syn to prevent its aggregation in vitro Both sHsps are very effective inhibitors of alpha-syn aggregation PMID: 27587396
    50. This study highlights a novel relation between serum pHSP27 and BMD in Indian women however, these findings need to be confirmed in larger studies PMID: 27241641

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  • 相关疾病：
    Charcot-Marie-Tooth disease 2F (CMT2F); Neuronopathy, distal hereditary motor, 2B (HMN2B)
  • 亚细胞定位：
    Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    Small heat shock protein (HSP20) family
  • 组织特异性：
    Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal f
  • 数据库链接：

    HGNC: 5246

    OMIM: 602195

    KEGG: hsa:3315

    STRING: 9606.ENSP00000248553

    UniGene: Hs.520973