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  4. Phospho-ITGA4 (S1027) Antibody

Phospho-ITGA4 (S1027) Antibody

  • 货号：
    CSB-PA011259
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    P13612
  • 基因名：
  • 别名：
    269C wild type antibody; Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody; CD49 antigen like family member D antibody; CD49 antigen-like family member D antibody; CD49d antibody; IA4 antibody; Integrin alpha 4 antibody; Integrin alpha 4 subunit antibody; Integrin alpha IV antibody; Integrin alpha-4 antibody; Integrin alpha-IV antibody; Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody; ITA4_HUMAN antibody; ITGA4 antibody; MGC90518 antibody; OTTHUMP00000205320 antibody; Very late activation protein 4 receptor, alpha 4 subunit antibody; VLA 4 subunit alpha antibody; VLA-4 subunit alpha antibody; VLA4 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from Human Integrin α4 around the phosphorylation site of S1027.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    IHC, IF, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:100-1:300
    IF 1:200-1:1000
    ELISA 1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1.
  • 基因功能参考文献：
    1. defines molecularly distinct subsets of articular cartilage that are enriched for osteochondral proteins PMID: 30194383
    2. study suggests a novel association of ITGA4 +3061A/G polymorphism with AD and its possible contribution to the disease pathology. PMID: 29769839
    3. The results of this study demonstrated that aggregation of hWJSCs into spherules alters their expression of ITGA4 and ITGA5. PMID: 29411210
    4. human ADAM11, ADAM23, and ADAM29 selectively support integrin alpha4-dependent cell adhesion. PMID: 28913673
    5. Recombinant human IL33 inhibited trophoblast invasion and adhesion, and decreased adhesion and invasionassociated molecules such as integrin alpha4beta1 and CD62L. PMID: 28765940
    6. study analyzes for the first time the expressions of epithelial-mesenchymal transition marker proteins - periostin, integrin alpha4, fibronectin - in metastatic castration-resistant prostate cancer PMID: 28836852
    7. ITGalpha4beta1 and TLR4 have roles in inducing fibrotic gene expression in response to fibronectin's EDA domain PMID: 28842322
    8. ITGA4 and PLCG1 are regulated by miR-30b in coronary artery endothelial cells from coronary atherosclerosis clinical samples. PMID: 27464494
    9. Low CD49D expression is associated with chronic lymphocytic leukaemia in comparison to small lymphocytic lymphoma. PMID: 29153094
    10. alpha-Integrin expression and function modulates presentation of cell surface calreticulin. PMID: 27310876
    11. AEbeta7 is of key relevance for gut trafficking of inflammatory bowel disease (IBD) CD8(+) T cells and CD4(+) Th9 cells in vivo and mainly retention might account for this effect. These findings indicate that blockade of alphaEbeta7 in addition to alpha4beta7 may be particularly effective in intestinal disorders with expansion of CD8(+) and Th9 cells such as IBD. PMID: 27543429
    12. sPLA2-IIA activates Integrin alphaVbeta3 and Integrin alpha4beta1 in an allosteric manner. (Review) PMID: 27864802
    13. Our results suggest that the analysis of alpha4beta7 integrin expression on circulating lymphocytes cannot be considered a reliable test for gastrointestinal involvement in CVID patients PMID: 28323147
    14. Glycosylation of V1V2 domain of HIV envelope protein determined its binding to alpha4ss7 integrin. PMID: 28577856
    15. confirmed CD49d as an independent negative OS prognosticator in CLL also in comprehensive models comprising the novel recurrent mutations. PMID: 27109509
    16. In colon cancer samples, there was a positive correlation between the expression of integrin alpha4 and VEGF-C. Integrin alpha4 and VEGF-C were significantly associated with the clinicopathological parameters (LMVD, Duke's stage, and lymph node metastasis). patients with high integrin alpha4 or VEGF-C expression had significantly shorter overall survival and tumor-free survival time. PMID: 26917449
    17. Macrophage tissue factor prothrombotic activity is regulated by integrin-alpha4/arf6 trafficking. PMID: 28495929
    18. gut-homing alpha4beta7 CD4(+) T cells and their functional subsets were profoundly depleted during acute HIV-1 infection. PMID: 26277899
    19. CD49d determination may be a useful tool to closely monitor Multiple Sclerosis activity in patients who interrupt Natalizumab PMID: 27496090
    20. Data show tht Rho kinase (ROCK) activity was increased by inhibiting integrin alpha4 with human umbilical vein endothelial cells (HUVECs) and hypoxia. PMID: 26448639
    21. These data showed that Mycobacterium tuberculosis ESAT-6 and ESAT-6/CFP-10 fusion proteins could induce adhesion of macrophages to fibronectin through alpha4beta1 integrin. PMID: 25081983
    22. This review gives an up to date insight into the multiple functional role of VLA-4 in cancer and introduces this integrin as a promising target worthwhile to attract attention in biomedical cancer research. PMID: 25564456
    23. Enhanced susceptibility of activated endocervical CD4+ T cells to HIV may be related to increased CCR5 expression by these cell subsets, but did not appear to be due to direct interaction of integrins a4b7 or a4b1 with HIV envelope. PMID: 25872482
    24. knockdown of PTTG1 increased expression of integrin alpha 4 (ITGA4), ITGA5, and integrin beta 1 (ITGB1); otherwise, RhoA expression was significantly decreased PMID: 26900962
    25. ITGA4 expression enhances metastasis in MYCN low neuroblastoma. PMID: 25973900
    26. Data show that a small molecule inhibitor of the MYC transcription factor can be an effective anticancer agent when delivered using a integrin alphavbeta3 and VLA-4-targeted nanotherapy approach. PMID: 25824336
    27. Collectively, these data suggest a role for alpha4beta7 integrin in HIV infection that is influenced by both viral and host factors including the sequence of the HIV gp120 alpha4beta7 binding motif, the cytokine milieu and bacterial vaginosis in the genital tract. PMID: 26105197
    28. Glioma cells induced the migration of BMSCs by promoting the expression of integrin a4. PMID: 26034878
    29. CD49d and CD26 have independent prognostic value and we suggest its use as a part of routine panel for prognostic stratification of CLL. PMID: 26142332
    30. Hypermethylation of ITGA4 promoter is increased in inflamed colon tissue. PMID: 25902909
    31. Regulatory CD4 T cells (Treg) cells in co-infected patients present phenotypic alterations and might have dysfunction marked by low expression of Foxp3 and increased expression of molecules not frequently seen on Treg cells, such as CD49d. PMID: 26329520
    32. High VLA-4 expression was independently associated with a high probability of complete remission in acute myeloid leukemia patients. PMID: 26155911
    33. Results show that integrin activation is absolutely required for OPN binding to the alpha4 integrin suggesting that OPN has very low affinity for the alpha4 integrin on human leukocytes under physiological conditions. PMID: 25446551
    34. alpha4beta7 serves as an attachment factor for some HIV-1 strains. PMID: 25527342
    35. Selective inhibition of VLA-4 expression on B cells impedes central nervous system accumulation of B cells and reduces susceptibility to experimental autoimmune encephalomyelitis. PMID: 25712734
    36. The carbon monoxide signaling pathway can rapidly down-modulate binding of the VLA-4 -specific ligand. PMID: 25367365
    37. CXCR4 and CD49d are important modulators of prognosis in chronic lymphocytic leukemia patients. PMID: 25349153
    38. Proinflammatory secreted phospholipase A2 type IIA (sPLA-IIA) induces integrin activation through direct binding to a newly identified binding site (site 2) in integrin alphaVbeta3, integrin alphavbeta1, and integrin alpha4beta1. PMID: 25398877
    39. LFA-1 and CR4 alpha chain phosphorylation is needed for chemokine-induced cross-talk to VLA-4 PMID: 25278023
    40. HIV-1 virions and many gp120s lack detectable alpha4beta7 binding activity. PMID: 25008916
    41. lymphocyte trafficking into the CNS under VLA-4 blockade can occur by using the alternative adhesion molecules, PSGL-1 and MCAM, the latter representing an exclusive pathway for TH17 cells to migrate over the blood-brain barrier PMID: 25135296
    42. DNA methylation of integrin alpha4 may be a poor prognostic factor which affects undifferentiated histologic change of breast cancer. PMID: 24756760
    43. Disruption of the hydrophobic contacts induces the active conformation of integrin alpha 4 beta 7. PMID: 24802248
    44. Pharmacological inhibition of the VLA-4/vascular adhesion molecule-1 axis in experimental stroke was ineffective PMID: 24743435
    45. Data indicate that CD47 in cis interactions regulate LFA-1 (integrin alphaLbeta2) and VLA-4 (integrin alpha4beta1) integrin affinity, and this process plays a substantial role in the adhesion of T-cells. PMID: 24006483
    46. VCAM-1/VLA-4-dependent activation of NF-kappaB has a role in mediating chemoresistance in leukemia cells PMID: 24599548
    47. Two conserved disulfide bonds located at integrin alpha4C589-C594 and beta7C494-C526 activated alpha4beta7. PMID: 23986478
    48. Only rotavirus specific CD4 T cells expressed intestinal homing receptors alpha4beta7 and CCR9. PMID: 24606696
    49. Data suggest CD38 (CD38 antigen (p45) protein) and CD49d (alpha4 Integrin; very late antigen-4 alpha) are more than just markers of an aggressive chronic lymphocytic leukemia (CLL) cell type and play functional roles in pathobiology of CLL. [REVIEW] PMID: 24288111
    50. Expression of the VLA-4-stimulating factor sequence may help to predict melanoma prometastatic risk. PMID: 23938462

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Integrin alpha chain family
  • 数据库链接：

    HGNC: 6140

    OMIM: 192975

    KEGG: hsa:3676

    STRING: 9606.ENSP00000380227

    UniGene: Hs.440955