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Phospho-MAP2K2 (Thr394) Antibody

  • 货号：
    CSB-PA062510
  • 规格：
    ￥2454
  • 图片：
    • Western blot analysis of extracts from HepG2 and Hela cells untreated or treated with UV using MEK-2(Phospho-Thr394) Antibody.
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2(Phospho-Thr394) Antibody(left) or the same antibody preincubated with blocking peptide(right).
    • Immunofluorescence staining of methanol-fixed Hela cells using MEK-2(Phospho-Thr394) Antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) MAP2K2 Polyclonal antibody
  • Uniprot No.：
    P36507
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Peptide sequence around phosphorylation site of threonine 394 (P-G-T(p)-P-T) derived from Human MEK-2.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC,IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
    IHC 1:50-1:100
    IF 1:100-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation.
  • 基因功能参考文献：
    1. Data show that SAM and SH3 domain containing 1 protein (SASH1) binds with mitogen-activated protein kinase kinase 2 (MAP2K2), and SASH1 mutations promote binding between SASH1 and MAP2K2. PMID: 28382689
    2. findings demonstrate the interaction of tRNA with MEK2 in pancreatic cancer cells and suggest that tRNA may impact MEK2 activity in cancer cells PMID: 27301426
    3. MEK2 was essential for the phosphorylation of MKK3/MKK6 and p38 MAPK that directly impacted on cyclin D1 expression. PMID: 27181679
    4. High MEK2 expression is associated with inflammation. PMID: 28178421
    5. there were significant decreases in intercellular adhesion molecules 1 (ICAM1), ezrin (EZR), mitogen-activated protein kinase kinase 2 (MAP2K2), and nitric oxide synthase 3 (NOS3) gene expressions in metabolic syndrome patients. PMID: 26956845
    6. The patient showed a paternally inherited 16p13.11 microduplication and a de novo 19p13.3 microdeletion involving the mitogen-activated protein kinase kinase 2 gene (MAP2K2), in which mutations cause the cardio-facio-cutaneous (CFC) syndrome PMID: 27751966
    7. We report on a fourth familial case with transmission of CFC syndrome from father to son due to a novel heterozygous sequence change c.376A>G (p.N126D) in exon 3 of MEK2 gene. PMID: 25487361
    8. MK2 attenuates dendritic cell-mediated Th1 differentiation and autoimmune encephalomyelitis. PMID: 26078274
    9. Our cohort of seven individuals with MEK2 deletions has overlapping features associated with RASopathies. PMID: 23379592
    10. Both ACE inhibition and MEK1/2 inhibition have beneficial effects on left ventricular function in Lmna(H222P/H222P) mice and both drugs together have a synergistic benefit when initiated after the onset of left ventricular dysfunction. PMID: 25218145
    11. we report familial patients with multiple cafe au lait spots and Noonan syndrome-like facial features who carried mutations in MAP2K2. PMID: 24311457
    12. osteosarcoma patients whose tumors expressed pMEK2 had a poorer clinical outcome than those whose tumors did not. PMID: 22935974
    13. MEK2 regulates ribonucleotide reductase activity through functional interaction with ribonucleotide reductase small subunit p53R2. PMID: 22895183
    14. E-cadherin is necessary for localization of DLG1 but not phosphorylated MEK2 to the midbody ring during cytokinesis. PMID: 22185284
    15. Mycobacterium tuberculosis lipomannan blocks TNF biosynthesis by regulating macrophage MAPK-activated protein kinase 2 (MK2) and microRNA miR-125b. PMID: 21969554
    16. hDlg acts as a MEK2-specific scaffold protein for the ERK signaling pathway. PMID: 21615688
    17. Data report the full-length structure of MEK2 obtained by homology modeling and molecular dynamics simulations. PMID: 21509657
    18. In the absence of other MKK, MEK2 is sufficient for SK-MEL-28 cell proliferation and anchorage-dependent growth. PMID: 21365009
    19. ETS1 is probably mediating high CIP2A expression in human cancers with increased EGFR-MEK1/2-ERK pathway activity PMID: 21445343
    20. familial inheritance of cardiofaciocutaneous syndrome with MEK2 mutation PMID: 21178588
    21. MEK1 and MEK2 play a part in the induction of the proinflammatory cytokine. PMID: 20837746
    22. This first reported case of a vertically transmitted functional Cardio-facio-cutaneous syndrome MEK mutation. PMID: 20358587
    23. MEK2 activity ad dual-phosphorylation were undetectable in expanding and self-renewing hematopoietic progenitors (HP). Adding IL-3, inducing maturation and cell death in HP, led to sustained high levels of MEK2 activity and dual-phosphorylation. PMID: 12032872
    24. MK2 phosphorylates TSC2, which creates a 14-3-3 binding site and thus regulates the cellular function of the TSC2 tumor suppressor protein PMID: 12582162
    25. MAPK activated protein kinase-2 mediates both ERK- and p38 MAPK-dependent neutrophil responses. PMID: 14499342
    26. HuR and MK2 in regulating the expression of uPA and uPAR genes at the posttranscriptional level PMID: 14517288
    27. Results suggest a physiological link between beta-dystroglycan and mitogen-activated protein kinase kinase 2 (MEK2), and localize MEK with dystroglycan in membrane ruffles. PMID: 15071496
    28. The ability of constitutively-active human MEK2 to stimulate ERK phosphorylation and to induce the neoplastic transformation of NIH 3T3 cells required the integrity of the D-site was found. PMID: 15979847
    29. These data suggest a role for mitochondrially generated reactive oxygen species and Ca(2+) in the redox cell signaling path-ways, leading to ERK activation and adaptation of the pathological stress mediated by oxidized lipids such as lysoPC. PMID: 16651638
    30. 3 novel mutations for MEK2 (L46_E55del, K61T, A62P) were identified in 15 patients with cardio-facio-cutaneous syndrome. PMID: 17704260
    31. Spectrum of MEK2 gene mutations in cardio-facio-cutaneous syndrome and genotype-phenotype correlations PMID: 19156172
    32. MEK2 interacts with ERK1. This interaction is mediated via a conserved N-terminal docking site in MEK2. PMID: 11134045

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  • 相关疾病：
    Cardiofaciocutaneous syndrome 4 (CFC4)
  • 亚细胞定位：
    Cytoplasm. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
  • 数据库链接：

    HGNC: 6842

    OMIM: 601263

    KEGG: hsa:5605

    STRING: 9606.ENSP00000262948

    UniGene: Hs.465627