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  4. Phospho-MAP3K11 (S674) Antibody

Phospho-MAP3K11 (S674) Antibody

  • 货号：
    CSB-PA000754
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of NIH-3T3, cells using Phospho-MLK3 (S674) Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    Q16584
  • 基因名：
  • 别名：
    2610017K16Rik antibody; EC 2.7.11.25 antibody; M3K11_HUMAN antibody; Map3k11 antibody; MEKK11 antibody; MGC17114 antibody; Mitogen-activated protein kinase kinase kinase 11 antibody; Mixed lineage kinase 3 antibody; Mixed lineage protein kinase 3 antibody; Mlk-3 antibody; Mlk3 antibody; Protein tyrosine kinase PTK1 antibody; PTK1 antibody; RHOE antibody; SH3 domain containing proline rich kinase antibody; SPRK antibody; Src-homology 3 domain-containing proline-rich kinase antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from Human MLK3 around the phosphorylation site of S674.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    ELISA 1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
  • 基因功能参考文献：
    1. These results suggest that in the early response to stressful stimuli, MLK4beta-MLK3 binding is important for regulating MLK3 activity and MAPK signalling, and after prolonged periods of stress exposure, MLK4beta and MLK3 proteins decline via CHIP-dependent degradation. PMID: 28757353
    2. In summary, this investigation identifies an EGFR-DOCK180-RAC1-MLK3-JNK signaling axis that drives glioblastoma cell migration and dissemination.IMPLICATIONS: On the basis of these findings, MLK3 emerges as a potential therapeutic target for the treatment of glioblastoma PMID: 28487380
    3. chronic hypoxia can reduce MLK3 expression in a posttranscriptional regulatory manner. PMID: 28555332
    4. Data indicate that BTG2, MAP3K11, RPS6KA1 and PRDM1 as putative targets of microRNA miR-125b. PMID: 27613090
    5. Increased expression of MAP3K11 is associated with esophageal cancer. PMID: 26717044
    6. MLK3 serves as a common upstream kinase of AMPK and JNK and functions as a direct upstream kinase for AMPK independent of LKB1 PMID: 25874865
    7. MLK3 represents a newly recognized integral component of HER2 biology in HER2+ breast tumors. PMID: 26152725
    8. MLK3 is a critical factor controlling the activity of kinase networks that control the cellular responses to different concentrations of reactive oxygen species. PMID: 24894995
    9. Signaling pathways associated with the Pro252His mutation in MLK3 are located in the kinase domain which is an important domain for the regulation of downstream signaling pathways. PMID: 24628919
    10. CHIP modulates MLK3 protein levels in response to Geldanamycin and stress stimuli, and CHIP-dependent regulation of MLK3 is required for suppression of SKOV3 ovarian cancer cell invasion. PMID: 24912674
    11. Data indicate URMC-099 as an orally bioavailable, potent mixed lineage kinase 3 MLK3 inhibitor. PMID: 24044867
    12. NS5A targets MLK3 with multiple downstream consequences for both apoptosis and K(+) homeostasis. PMID: 23857585
    13. Lysine 63-linked ubiquitination modulates mixed lineage kinase-3 interaction with JIP1 scaffold protein in cytokine-induced pancreatic beta cell death PMID: 23172226
    14. High MAP3K11 expression is associated with prostate cancer. PMID: 22761715
    15. Findings suggest that the MLK3-JNK-paxillin signaling axis may represent a potential prognostic marker in breast cancer metastasis. PMID: 22700880
    16. Data suggest that MEKK2 and MLK3 represent untargeted kinases in tumor biology for potential therapeutic development. PMID: 22139075
    17. The findings establish MLK3 as an important regulator of MMP expression and invasion in ovarian cancer cells. PMID: 22652451
    18. These findings collectively suggest that the MLK3-Pin1 signaling cascade plays a critical role in regulating the cell cycle, centrosome numbers, and oncogenesis. PMID: 22566623
    19. Data show that HBx can induce HepG2 cell apoptosis via a novel active MLK3-MKK7-JNKs signaling module to upregulate FasL protein expression. PMID: 22509080
    20. co-expression of MLK3 and beta-catenin resulted in significant G(2)/M arrest. PMID: 21880738
    21. These results establish merlin as a potent inhibitor of mixed lineage kinase 3, extracellular signal-regulated kinase, and c-jun N-terminal kinase activation in cancer. PMID: 20890305
    22. MLK3 is critical for breast cancer cell migration and promotes cell transformation. PMID: 20514022
    23. Studies indicate that the MLK3/JNK1 axis mediates G17-induced gastric cancer cell migration, which can be targeted for designing novel therapeutic strategies for treating gastric malignancies. PMID: 20150185
    24. Novel proteolytic processing of MLK3 may negatively control MLK3 signalling to JNK. PMID: 20158498
    25. MLK3 mutations is associated with the mismatch repair deficiency in gastrointestinal cancer. PMID: 19955118
    26. Estradiol inhibits the proapoptotic function of MLK3 as a mechanism to limit cytotoxic drug-induced death of ER(+) breast cancer cells. PMID: 20145118
    27. MLK3 functions to limit IKK activity, and depleting MLK3 helps protect cells from etoposide-induced cell death through activation of IKK-dependent signaling. PMID: 19782705
    28. MLK3 may be a negative regulator of the growth-promoting and transforming properties of Rac1 PMID: 11713255
    29. Twelve in vivo MLK3 phosphorylation sites have been identified. PMID: 11969422
    30. negative regulation by protein kinase B leads to cell survival PMID: 12458207
    31. ceramide and TNF-alpha are agonists PMID: 12504027
    32. MLK3 is phosphorylated by Akt2, which results in the disassembly of the JNK complex bound to POSH and down-regulation of the JNK signaling pathway PMID: 14504284
    33. MLK3 signaling requires Hsp90/p50cdc37 PMID: 15001580
    34. These observations support a role for mixed lineage kinases, including MLK3, in the TGF-beta1-induced cell death mechanism. PMID: 15069087
    35. MLK3 is required for mitogen activation of B-Raf, ERK and cell proliferation. PMID: 15258589
    36. The MLK3 might be a unique target to selectively inhibit transformed cell proliferation by disrupting mitotic spindle formation resulting in mitotic arrest. PMID: 15923109
    37. Cdc42 induces activation loop phosphorylation and membrane targeting of mixed lineage kinase 3 PMID: 16253996
    38. Merlin and MLK3 can interact in situ and merlin can disrupt the interactions between B-Raf and Raf-1 or those between MLK3 and either B-Raf or Raf-1. PMID: 16537381
    39. JNK-mediated feedback phosphorylation of MLK3 regulates its activation and deactivation states by cycling between Triton-soluble and Triton-insoluble forms PMID: 16687404
    40. a direct link between GSK-3beta and MLK3 activation in a neuronal cell death pathway and identify MLK3 as a direct downstream target of GSK-3beta. PMID: 17711861
    41. p38 MAPK inhibitors SB202190 and SB203580 activated JNK via MLK-3/MKK7 pathway. PMID: 18222647
    42. findings demonstrate a scaffolding role for MLK3 in controlling the extent of Rho activation that modulates cell migration PMID: 18851832
    43. K48-linked ubiquitination directs Mixed lineage kinase 3 for proteosomal degradation while K63-linked ubiquitination is important for Mixed lineage kinase 3 kinase activity. PMID: 19586614

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Location is cell cycle dependent.
  • 蛋白家族：
    Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
  • 组织特异性：
    Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
  • 数据库链接：

    HGNC: 6850

    OMIM: 600050

    KEGG: hsa:4296

    STRING: 9606.ENSP00000309597

    UniGene: Hs.502872