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  4. Phospho-NCOA2 (S736) Antibody

Phospho-NCOA2 (S736) Antibody

  • 货号：
    CSB-PA006558
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    Q15596
  • 基因名：
  • 别名：
    bHLHe75 antibody; Class E basic helix-loop-helix protein 75 antibody; glucocorticoid receptor interacting protein 1 antibody; GRIP 1 antibody; GRIP1 antibody; hTIF2 antibody; MED1 antibody; MGC138808 antibody; NCoA 2 antibody; NCoA-2 antibody; Ncoa2 antibody; NCOA2_HUMAN antibody; Nuclear receptor coactivator 2 antibody; Oncogene FGR antibody; p160 antibody; SRC 2 antibody; src2 antibody; Steroid receptor coactivator 2 antibody; TIF 2 antibody; TIF2 antibody; Transcriptional intermediary factor 2 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from Human GRIP-1 around the phosphorylation site of S736.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as RORA coactivator to specifically modulate G6PC1 expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer.
  • 基因功能参考文献：
    1. Using quantitative biochemical, biophysical, and solution structural methods study shows that ligand and DNA cooperatively recruit the intrinsically disordered steroid receptor coactivator-2 (SRC-2/TIF2/GRIP1/NCoA-2) receptor interaction domain to peroxisome proliferator-activated receptor gamma-retinoid X receptor alpha (PPARgamma-RXRalpha) heterodimer and reveal the binding determinants of the complex. PMID: 28890360
    2. T3 promotes differentiation towards chondrocytes-like cells in our in vitro model, that this differentiation is mediated by steroid receptor co-activator 2 (SRC2) and does not induce hypertrophy PMID: 26869487
    3. Pancreatic involvement occurs in mesenchymal chondrosarcoma harboring the HEY1-NCOA2 gene fusion. PMID: 27544802
    4. Data suggest that steroid receptor coactivators (NCOA1, NCOA2, NCOA3) are over-expressed in a number of hormone-dependent cancers where they promote tumor growth, invasion, metastasis, and chemo-resistance; with their multiple roles in cancer, steroid receptor coactivators are promising targets for development of antineoplastic agents that can interfere with their function. [REVIEW] PMID: 28390937
    5. SRC-2 may exhibit oncogenic or tumor suppressor activity depending on the target genes and nuclear receptors that are expressed in distinct tissues PMID: 28273073
    6. NCOA2ETV4 protein would contain the helixloophelix, PAS_9 and PAS_11, CITED domains, the SRC1 domain of NCOA2 and the ETS DNAbinding domain of ETV4. PMID: 27633981
    7. Altered expression of TIF2 may play a role in adenomyosis development and treatment outcome with levonorgestrel-releasing intrauterine system. PMID: 26040939
    8. evaluating if NCOA2 relative copy-number gain presents prognostic value for prostate cancer PMID: 26799514
    9. Report NcoA2-regulation of the AhR-ARNT-HIF-1a interaction. PMID: 26350169
    10. Data suggest that LRH1/NR5A2 (liver receptor homologue-1) exhibits phospholipid-mediated allosteric control of protein-protein binding interface in interactions with TIF2 and SHP (co-repressor; small heterodimer partner protein). PMID: 26553876
    11. NCOA2 is a novel negative growth regulatory gene repressing the Wnt/beta-catenin pathway in colorectal cancer, where recurrent fusion with LACTB2 contributes to its disruption. PMID: 25823027
    12. Suggest a possible association between NCOA2 rs10504473 polymorphism and obesity in Chinese Han population. PMID: 26261634
    13. miR-137 has a role in targeting p160 steroid receptor coactivators SRC1, SRC2, and SRC3 and inhibits cell proliferation PMID: 26066330
    14. results suggest that CCNC temporarily protects SRC-2 against degradation and this event is involved in the transcriptional regulation of SRC-2 cell cycle target genes. PMID: 25986860
    15. Data suggest that over-stimulating the steroid seceptor coactivators SRC-1, SRC-2, and SRC-3 oncogenic program can be an effective strategy to kill cancer cells. PMID: 26267537
    16. SRC-2 is a prominent metabolic coordinator of cancer metastasis. PMID: 25664849
    17. Because deregulation of endometrial SRC-2 expression has been associated with common gynecological disorders of reproductive-age women, this signaling pathway, involving SRC-2 and PFKFB3. PMID: 24204309
    18. the PAX3-NCOA2 fusion gene has a dual role in the tumorigenesis of rhabdomyosarcoma PMID: 24213582
    19. increased levels of SRC-2 impairs murine endometrial function PMID: 24905738
    20. NCOA1 plays a necessary role in E2-induced CXCL12 expression and NCOA2 is required for P4 to inhibit the E2-induced CXCL12 production in normal and ectopic endometrium. PMID: 24586072
    21. Transcription factor 23 (Tcf23), a basic-helix-loop-helix transcription factor, is a new progesterone-induced target gene that requires SRC-2 for full induction. PMID: 24571987
    22. The combination of NCOA2 FISH and Stat6 IHC proved effective for the differential diagnosis of soft-tissue angiofibroma, even when using small biopsy specimens. PMID: 24856853
    23. MOZ-TIF2/BRPF1 complex upregulates HOX genes mediated by MOZ-dependent histone acetylation, leading to the development of leukemia. PMID: 24258712
    24. Data show the networks of interactions that connect retinoid X receptor alpha (RXRalpha) agonists to coactivator GRIP1 binding. PMID: 24187139
    25. Downregulation of steroid receptor coactivator-2 modulates estrogen-responsive genes and stimulates proliferation of mcf-7 breast cancer cells. PMID: 23936147
    26. HBO1, but not SRC-2, expression was reduced in testes of patients with androgen insensitivity syndromes compared to normal testes. PMID: 23707616
    27. The identification of HEY1-NCOA2 can be used as an auxiliary diagnostic tool to differentiate meningeal hemangiopericytoma from mesenchymal chondrosarcoma. PMID: 24124145
    28. Recurrent NCOA2 gene rearrangements is associated with congenital/infantile spindle cell rhabdomyosarcoma PMID: 23463663
    29. Transactivation of glucocorticoid receptor-interacting protein 1 transactivation was stimulated to a similar degree by promyelocytic leukemia protein. PMID: 23542129
    30. cAMP response element-binding protein (CREB) downregulates GRIP1 and is necessary for the PKA-stimulated degradation of GRIP1, which leads to changes in the expression of a subset of genes regulated by estrogen receptor-alpha in MCF-7 breast cancer cells. PMID: 23462962
    31. the cellular accumulation of IRF-1 may represent a potential molecular mechanism mediating altered cellular response to GC through the depletion of GRIP-1 from the GR transcriptional regulatory complexes PMID: 19805480
    32. dissect the GRIP1:Suv4-20h1 interaction in vitro and in vivo and examine its potential involvement in hormone-dependent transcriptional regulation by GR PMID: 19074285
    33. The initial stimulation of GRIP1 coactivator function is followed by an increased turnover and subsequent degradation of GRIP1 protein. PMID: 18499756
    34. CoCoA uses different combinations of functional domains in its synergistic coactivator function with beta-catenin or GRIP1 PMID: 16344550
    35. modulation of nuclear receptor interaction domain by covalent attachment of SUMO-1 PMID: 12060666
    36. The current study adds further support for the use of HEY1-NCOA2 fusion as a valid diagnostic marker for Mesenchymal chondrosarcoma PMID: 23252872
    37. Overexpression of SRC-1 (NCOA1) and TIF-2 (NCOA2) increases estrogen-induced gene expression. PMID: 12403846
    38. Binding of the N-terminal region of TIF2 to the AF1 domain of the glucocorticoid receptor changes both its conformation and transcriptional activity. PMID: 23132854
    39. The crystal structure of RXRA-ligand binding domain:9-cis-retinoic acid:GRIP1 is reported at 2.05 A. PMID: 21049972
    40. findings suggest that TIF2 is a novel binding partner for nuclear EGFR and is involved in regulating its target gene expression. PMID: 22581837
    41. a substantial subset of soft tissue angiofibroma is characterized by a t(5;8)(p15;q13), resulting in the expression of in-frame AHRR/NCOA2 and NCOA2/AHRR fusion transcripts. PMID: 22337624
    42. Univariate survival analysis revealed that high tif2 expression was associated with worse prognosis in astrocytic brain tumors PMID: 21735116
    43. The novel HEY1-NCOA2 fusion appears to be the defining and diagnostic gene fusion in mesenchymal chondrosarcomas PMID: 22034177
    44. Genetic aberrations and dysregulation in expression of p160/SRC coactivators and the ANCCA in breast cancer, prostate cancer, and other nonhormone-responsive cancers, are reviewed. PMID: 20374707
    45. Treatment of THP-1 cells with coenzyme Q10 significantly decreased expression of NCOA2. PMID: 21370964
    46. we detected NCOA2 mutations neither in prostate cancers nor in other cancers PMID: 21492233
    47. Distinctive functions of p160 steroid receptor coactivators in proliferation of an estrogen-independent, tamoxifen-resistant breast cancer cell line. PMID: 21059860
    48. SRC-2 and SRC-3 concomitantly promote human adipocyte differentiation by attenuating phospho-PPARgamma-Serine114 and modulating PPARgamma cellular heterogeneity. PMID: 21220509
    49. the hepatic AMPK-SRC-2 axis as an energy rheostat PMID: 21195347
    50. MOZ-TIF2 oncogenic fusion protein suppresses transcription by nuclear receptors and p53 PMID: 15657427

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  • 相关疾病：
    Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation.
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    SRC/p160 nuclear receptor coactivator family
  • 数据库链接：

    HGNC: 7669

    OMIM: 601993

    KEGG: hsa:10499

    STRING: 9606.ENSP00000399968

    UniGene: Hs.446678