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  4. Phospho-PRKD2 (S876) Antibody

Phospho-PRKD2 (S876) Antibody

  • 货号：
    CSB-PA000648
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of HELA cells using Phospho-PKD2 (S876) Polyclonal Antibody
    • Western Blot analysis of HELA cells using Phospho-PKD2 (S876) Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    Q9BZL6
  • 基因名：
  • 别名：
    DKFZp586E0820 antibody; HSPC187 antibody; KPCD2_HUMAN antibody; nPKC D2 antibody; nPKC-D2 antibody; PKD2 antibody; PRKD 2 antibody; Prkd2 antibody; Protein kinase D2 antibody; Serine/threonine protein kinase D2 antibody; Serine/threonine-protein kinase D2 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from Human PKD2 around the phosphorylation site of S876.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:40000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion. May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. In response to oxidative stress, is phosphorylated at Tyr-438 and Tyr-717 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B. In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77. Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens. In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF-kappa-B-dependent pathway. During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane. Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis. In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN. Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells. Plays a regulatory role in angiogenesis and tumor growth by phosphorylating a downstream mediator CIB1 isoform 2, resulting in vascular endothelial growth factor A (VEGFA) secretion.
  • 基因功能参考文献：
    1. the knockdown of PKD2 reduces cell death and promotes polyploidization induced by PMA. PMA/PKD2-mediated necrosis via PARP cleavage involves both SOD1-dependent and -independent pathways. PMID: 26241492
    2. The results implied that PKD2 silencing might inhibit migration of MCF7/DOX cells without affecting chemoresistance significantly PMID: 25874490
    3. PRKD2 silencing induces glioma cell senescence via p53-dependent and -independent pathways. PMID: 24463355
    4. Both PKD2 and GOLPH3 play important roles in the progression of human gliomas by promoting cell proliferation. PMID: 25218347
    5. PKD2 controls secretion of MMP7 and 9 in an isoform-specific manner PMID: 24336522
    6. PRKD2 mediates BAZF gene expression by VEGF-A stimulation. PMID: 23515950
    7. PRKD2 is a potential target to interfere with glioblastoma cell migration and invasion, two major determinants contributing to recurrence of glioblastoma after multimodality treatment. PMID: 23562655
    8. results suggest that PKD2 is involved in the regulation of PP2A activity in activated T cells through phosphorylation of Ser171 of SET PMID: 23251465
    9. found that AMAP1 had the ability to bind directly to PRKD2 and hence to make a complex with the cytoplasmic tail of the beta1 subunit PMID: 22734003
    10. upregulation of PKD2 expression may determine the behavior of gastric tumor cells, which promotes invasive phenotype and could result in general poor prognosis. PMID: 22217708
    11. Tyrosine phosphorylation of PKD2 is required for IFNalpha-stimulated activation of this kinase as well as for efficient serine phosphorylation and degradation of IFNAR1 and ensuing restriction of the extent of cellular responses to IFNalpha. PMID: 21865166
    12. PKD2 is a common signaling target downstream of various agonist receptors in platelets and G(q)-mediated signals along with calcium and novel PKC isoforms, in particular, PKCdelta activate PKD2 in platelets. PMID: 21736870
    13. Expression and kinase activity of PKD2 are required for the ligand-inducible stimulation of IFNAR1 ubiquitination and endocytosis and for accelerated proteolytic turnover of IFNAR1. PMID: 21173164
    14. PKD2 regulates hypoxia-induced VEGF-A expression/secretion by tumour cells and VEGF-A stimulated blood vessel formation and a essential mediator of tumour cell-endothelial cell communication and a target to inhibit angiogenesis in gastrointestinal cancers PMID: 20732914
    15. mechanism of activation by CCK(B)/gastrin receptor PMID: 12058027
    16. Data show that Bcr-Abl-induced activation of the nuclear factor kappaB cascade in myeloid leukemia cells is largely mediated by tyrosine-phosphorylated PKD2. PMID: 15604256
    17. Critical components of the PKD2 regulatory domain control phorbol ester binding, catalytic activity, and nucleocytoplasmic shuttling and reveal similarities but also striking differences compared to the published data for this domain in PKD1. PMID: 15975900
    18. PKD2 plays an important role in carcinoid tumor progression. PMID: 16899224
    19. PKD2 is involved in the production of IL-8, a potent proinflammatory chemokine, by epithelial cells. PMID: 16928771
    20. The data suggest that PKD2 is involved in IL-2 promoter regulation and cell death depending on its activity upon TCR stimulation in Jurkat cells. PMID: 17077180
    21. Neurotensin induces a striking increase in Hsp27 phosphorylation on Ser-82 in PANC-1 cells through convergent p38 MAPK, PKD, and PKD2 signaling. PMID: 17570131
    22. stimulation of P-selectin surface expression via PKCalpha-dependent PKD2 activation could be an important mechanism in the early onset of AII-initiated endothelial adhesiveness PMID: 17951978
    23. Signal transduction pathway is defined downstream of CCK2 receptor showing that CK1 delta and epsilon phosphorylate PKD2 at 3 sites, resulting in nuclear accumulation of PKD2 and phosphorylation of nuclear PKD2 substrates in human gastric cancer cells. PMID: 17962809
    24. expression and catalytic activity of PKD2 are required for the release of chromogranin A containing secretory vesicles PMID: 18262756
    25. PKD2 plays a pivotal role in endothelial cell proliferation and migration necessary for angiogenesis at least in part through modulation of the expression of vascular endothelial growth factor receptor-2 and fibroblast growth factor receptor-1 PMID: 19001381
    26. Lck regulated the activity of PKD2 by tyrosine phosphorylation, which in turn may have modulated the physiological functions of PKD2 during T cell receptor-induced T cell activation. PMID: 19192391
    27. Lysophosphatidylcholine activates a novel PKD2-mediated signaling pathway that controls monocyte migration. PMID: 19520973
    28. a transient association of PKD1 and PKD2 with NHERF-1 in live cells that is triggered by phorbol ester stimulation and, importantly, differs strikingly from the sustained translocation to plasma membrane. PMID: 19581308

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  • 亚细胞定位：
    Cytoplasm. Cell membrane. Nucleus. Golgi apparatus, trans-Golgi network.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, PKD subfamily
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 17293

    OMIM: 607074

    KEGG: hsa:25865

    STRING: 9606.ENSP00000291281

    UniGene: Hs.466987