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  4. Phospho-Pim1 (Tyr309) Antibody

Phospho-Pim1 (Tyr309) Antibody

  • 货号：
    CSB-PA174999
  • 规格：
    ￥2454
  • 图片：
    • Western blot analysis of extracts from HUVEC cells treated with PMA using Pim-1 (Phospho-Tyr309) Antibody.The lane on the right is treated with the antigen-specific peptide.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Mus musculus (Mouse) Pim1 Polyclonal antibody
  • Uniprot No.：
    P06803
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Peptide sequence around phosphorylation site of tyrosine 309(A-A-V(p)-W-S) derived from Human Pim-1.
  • 免疫原种属：
    Mus musculus (Mouse)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, another proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promotes cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B, induces 14-3-3 binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. Also phosphorylates and activates the ATP-binding cassette transporter ABCG2, allowing resistance to drugs through their excretion from cells. Promotes brown adipocyte differentiation.
  • 基因功能参考文献：
    1. Pim-1 is a novel kinase that phosphorylates cTnI primarily at Ser23/24 and Ser150 in cardiomyocytes, which in turn may modulate myofilament function under a variety of physiological and pathophysiological conditions. PMID: 29544221
    2. this study shows that Pim-1 dysregulation has developmental-stage-specific effects on B lymphopoiesis and early myeloid, but not erythroid progenitors PMID: 27287229
    3. Results show that elevated miR-15b as an early response to Dicer depletion silenced Pim-1 kinase resulting in mitochondrial dysfunction. Rescue experiments demonstrated that suppression of miRNA-15b induction in Dicer-deleted adult hearts result in marked amelioration of cardiac dysfunction. PMID: 23840532
    4. Pim-1L protects hepatic ABCA1 from lysosomal degradation by facilitating the physical interaction between ABCA1 and liver X receptor beta and subsequent stabilization of the ABCA1-Pim-1L complex and thereby regulates the circulating level of high-density lipoprotein. PMID: 27765770
    5. Pim1 kinase activity maintains airway epithelial integrity and protects against house dust mite-induced proinflammatory activation of the airway epithelium. PMID: 26453516
    6. Pim kinases prevent premature cardiac aging and maintain a healthy pool of functional mitochondria leading to efficient cellular energetics. PMID: 24916111
    7. Pim-1 kinase activity regulates hepatocyte growth factor-induced tumor cell migration, invasion, and cell scattering. PMID: 24777602
    8. these results identify Pim1 as a novel effector molecule sufficient to drive CD4( ) alphabeta T-cell development and survival in the absence of gammac cytokine receptor signaling. PMID: 23712827
    9. Our data suggest that Pim1 might contribute to progression rather than initiation in prostate neoplasia. PMID: 23565217
    10. Loss of PIM1 is associated with abnormal hematopoietic phenotypes. PMID: 23360755
    11. Pim-1 activity prevents Drp1 compartmentalization to the mitochondria and preserves reticular mitochondrial morphology in response to sI PMID: 23530233
    12. Pim1 kinase deletion and inhibition slows endothelial cell detachment by trypsinization and increases cell adhesion. PMID: 23202547
    13. Telomere elongation consistent with a young phenotype is observed following Pim-1 overexpression. PMID: 22915504
    14. demonstrated that over-expression of miR-16 mimics suppressed Pim-1 expression in FD-FLT3/ITD cells suggesting that increased miR-16 expression contributes to depletion of Pim-1 after FLT3 inhibition PMID: 22970245
    15. a novel regulatory axis involving Pim1 kinase and Runx3 in the control of food-induced allergic reactions through the regulation of T(H)2 and T(H)17 differentiation. PMID: 22944483
    16. Inhibition of Pim-1 kinase abolished desflurane-induced postconditioning & ischemic postconditioning. Pim-1 kinase mediates both processes in mice. PMID: 22385356
    17. Pim-1 kinase is involved in mucosal injury/inflammation. PMID: 22466098
    18. the important role of Pim1 kinase in the full development of allergen-induced airway responses PMID: 22074702
    19. Pim-1 expression correlates with lymphocyte proliferation and activation. PMID: 21974958
    20. Pim-1 kinase is upregulated by STAT3 activation in cardiac Sca-1+ cells and plays a pivotal role in EC differentiation both in vitro and in vivo. PMID: 21600215
    21. Targeting cap-dependent translation blocks converging survival signals by AKT and PIM 1, 2 kinases in lymphoma PMID: 21859846
    22. RAGE activation accounts for many features of Vascular remodeling diseases including VSMC proliferation and resistance to apoptosis by the activation of STAT3/Pim1/NFAT axis. PMID: 21680901
    23. Pim-1 sustains survival of rapidly proliferating cells by antiapoptotic and prometabolic effects that are independent of the mammalian target of rapamycin (mTOR) pathway. PMID: 21048108
    24. Data show that Pim-1 is able to cooperate with Skp2 to signal S phase entry. PMID: 20663873
    25. PDGF(bb)-induced proliferation of vascular smooth muscle cells is partly attributed to transcriptionally upregulated Pim-1. PMID: 19711112
    26. Pim-1 expression is sufficient to induce cytokine independence in murine hematopoietic cells, but is dispensable for BCR-ABL-mediated transformation. PMID: 12135666
    27. Pim-1 interacts with SOCS1 and SOCS3 and potentiates their inhibitory effects on STAT5, most likely via phosphorylation-mediated stabilization of the SOCS proteins PMID: 14764533
    28. Pim-1 is required for differentiation into vascular endothelium and smooth muscle cells. PMID: 14982870
    29. Pim-1 kinase stability is regulated by heat shock proteins and the ubiquitin-proteasome pathway PMID: 15798097
    30. Pim-1 expression may be regulated by progesterone during mammary development and Pim-1 associates with p21 in mammary epithelial cells PMID: 16712793
    31. Pim-1 kinase has a novel role in the control of self-renewal of embryonic stem cells. PMID: 17717068
    32. Pim-1 is a crucial facet of cardioprotection downstream of Akt PMID: 18037896
    33. Pim kinases play a key role in the v-Abl transformation, possibly via participating in modulation of SOCS-1 and via regulating the apoptotic signaling PMID: 18042805
    34. Nucleostemin expression in cardiomyocytes is induced by fibroblast growth factor-2 and accumulates in response to Pim-1 kinase activity. PMID: 18519946
    35. Pim-1 phosphorylates four Ser/Thr residues within the Runt domain and stabilizes RUNX3 protein, alterING the cellular localization of RUNX3 from the nucleus to the cytoplasm PMID: 18767071
    36. ALDH2 may effectively ameliorate alcohol-induced hepatic apoptosis and changes in Akt as well as Pim signalling. PMID: 19215238
    37. Pim-1 plays a distinct role in solid tumor formation PMID: 19528349
    38. PIM1 regulates surface CXCR4 expression in hematopoietic cells lines, and is a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. PMID: 19687226
    39. Pim-1 kinase mediates at least in part desflurane-induced preconditioning and IPC against myocardial infarction in mice PMID: 19934869

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  • 相关疾病：
    Frequently activated by provirus insertion in murine leukemia virus-induced T-cell lymphomas.
  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell membrane. Note=Mainly located in the cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, PIM subfamily
  • 数据库链接：

    STRING: 10090.ENSMUSP00000024811

    UniGene: Mm.405293