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  4. Phospho-SYK (Y348) Antibody

Phospho-SYK (Y348) Antibody

  • 货号：
    CSB-PA000697
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of 3T3 cells using Phospho-Syk (Y348) Polyclonal Antibody
    • Western Blot analysis of NIH-3T3 cells using Phospho-Syk (Y348) Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    P43405
  • 基因名：
  • 别名：
    EC 2.7.10.2 antibody; kinase Syk antibody; KSYK antibody; KSYK_HUMAN antibody; p72-Syk antibody; p72syk antibody; Spleen tyrosine kinase antibody; Syk antibody; Tyrosine protein kinase SYK antibody; Tyrosine-protein kinase SYK antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat,Monkey
  • 免疫原：
    Synthesized peptide derived from Human Syk around the phosphorylation site of Y348.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:10000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

引用文献

产品评价

靶点详情

  • 功能：
    Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR plays also a role in T-cell receptor signaling. Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade. Required for the stimulation of neutrophil phagocytosis by IL15. Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Involved in interleukin-3/IL3-mediated signaling pathway in basophils. Also functions downstream of receptors mediating cell adhesion. Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Plays also a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen. Together with CEACAM20, enhances production of the cytokine CXCL8/IL-8 via the NFKB pathway and may thus have a role in the intestinal immune response.
  • 基因功能参考文献：
    1. In this article, the s have shown that Natural killer cells can reduce their functional role through downregulation of Syk and Zap70 kinases. PMID: 29263215
    2. DFNA5 variant is associated with tobacco- and HPV-mediated oral oncogenesis. PMID: 30091681
    3. This study identifies a novel signaling involving SYK/c-MYC/MALAT1 as a promising therapeutic target for the treatment of Ewing sarcoma. PMID: 28336564
    4. Comparison of the distributions of tau pTyr18 and double-phosphorylated Syk in the transgenic mouse brain and human hippocampus showed that the phosphorylation of tyrosine 18 in tau already occurs at an early stage of tauopathy and increases with the progression of neurodegeneration. Syk appears unlikely to be a major kinase that phosphorylates tyrosine 18 of tau at the early stage of tauopathy. PMID: 28919467
    5. Results show that Syk activation occurs following Abeta deposition and the formation of tau pathological species. As Syk activation also promotes Abeta formation and tau hyperphosphorylation, results suggest that Alzheimer's disease pathological lesions may be self-propagating via a Syk dependent mechanism highlighting Syk as an attractive therapeutic target for the treatment of Alzheimer's disease. PMID: 28877763
    6. study reports that neutrophils constitutively express various Src family isoforms as well as Syk, and that inhibition of these protein tyrosine kinases selectively hinders inflammatory cytokine generation by acting posttranscriptionally PMID: 28512645
    7. expressed by immune cells and keratinocytes in skin lesions of cutaneous lupus erythematosus patients PMID: 26910509
    8. Cholesterol crystals activate specific cell signalling pathways which drive the production of inflammatory cytokines and degradative enzymes known to contribute to disease initiation and progression and are dependent on activation of Syk and PI3K PMID: 27356299
    9. Syk-induced signals in bone marrow stromal cell lines are mediated by phospholipase C gamma1 (PLCgamma1) in osteogenesis and PLCgamma2 in adipogenesis. PMID: 28786489
    10. AKT and 14-3-3 proteins down-regulate the activity of several BCR-associated components, including BTK, BLNK and SYK and also inhibit SYK's interaction with Importin 7 PMID: 27381982
    11. Expression of SYK and its splice variants was significantly lower in tumors with MSI, and in KRAS wild type, BRAF mutant and PTEN mutant tumors PMID: 28957395
    12. combined use of butyrate and highly specific Syk inhibitor BAY61-3606 does not enhance differentiation and apoptosis of colonocytes. Instead, BAY completely abolishes butyrate-induced differentiation and apoptosis in a Syk- and ERK1/2-dependent manner. PMID: 27293079
    13. Study demonstrates that redundant complexes formed by the mutant SYK(Y3F) protein allow it to maintain signaling to receptor-proximal interaction partners, resulting in proper initiation of BCR-proximal signals. PMID: 28760774
    14. The study identified the criteria for the design of binders that specifically address either the Syk or the Zap-70 Tandem Src Homology 2 Domains, tSH2. While Syk tSH2 has a rather broad substrate scope, ZAP-70 tSH2 required a proximal arrangement of the phosphotyrosine ligands in defined strand orientation. PMID: 28767218
    15. SYK mediates the actions of EPO and GM-CSF and coordinates with TGF-beta in erythropoiesis. PMID: 28131718
    16. study demonstrates that high SYK expression is a common feature of CD21(low) B cells independent of the underlying disorder, and that this high expression is sufficient to drive constitutive phosphorylation of SYK and its immediate targets Bruton's tyrosine kinase and phospholipase Cgamma2 PMID: 28468967
    17. IgG (mAb)-opsonized, inactivated Francisella tularensis LVS enhances macrophage and dendritic cell IL-1beta responses in a TLR2- and FcgammaR-dependent fashion. FcgammaR-mediated Syk activation leads to NLRP3 inflammasome-dependent IL-1beta production in macrophages. PMID: 27365531
    18. High SYK expression is associated with lymphangioleiomyomatosis. PMID: 28202529
    19. NKp65 utilizes a hemi-immunoreceptor tyrosine-based activation motif -like motif for cellular activation that requires Syk, although Syk appears not to be recruited to NKp65. PMID: 28082678
    20. Syk is a key regulator of Hoxa9/Meis1-driven acute myeloid leukemia. PMID: 28399410
    21. Data show that spleen tyrosine kinase (SYK) and proto-oncogene protein c-akt (AKT1) proteins were increased in the cytoplasm of the cells forming Mallory-Denk bodies. PMID: 28089901
    22. B cell receptor signaling component, SYK, caused PAX5 tyrosine phosphorylation in vitro and in cells. Transcriptional repression on the BLIMP1 promoter by PAX5 was attenuated by this phosphorylation. PMID: 27181361
    23. Pharmacological inhibitors of SYK activity significantly reduced the engulfment of oxLDL microbeads in the presence of serum factors, but had little effect on IgG phagocytosis. PMID: 27510553
    24. our data strongly suggest that AQCA-mediated suppression of inflammatory responses could be managed by a direct interference of signaling cascades including IRAK and Syk, linked to the activation of NF-kappaB and AP-1. PMID: 27338330
    25. a potential link between the upregulation of Syk and VEGF-C expression and lung adenocarcinoma. PMID: 27461624
    26. The lack of Syk mRNA expression in lung cancer play an important role in angiogenesis. PMID: 27461628
    27. Overall novel mutations in SYK gene and in silico analysis revealed that these mutations are crucial and might be responsible for altered expression of SYK. PMID: 26889814
    28. this study shows that SYK increased MUC5AC expression via ERK2 and p38 MAPK signaling pathways in airway epithelial cells PMID: 26980390
    29. SYK, LYN and PTPN6 were markedly elevated in atherosclerotic plaques of carotid atherosclerosis patients. PMID: 26742467
    30. Syk inhibitor suppressed the phosphorylation of band 3 also preventing serine phosphorylation changes and hemolysis. PMID: 27034738
    31. SYK expression was down-regulated in laryngeal squamous cell carcinoma, which was closely correlated with cancer growth and lymph node metastasis. SYK up-regulation was able to inhibit the invasion and metastasis of LSCC. PMID: 26884848
    32. TNF activates Mule by inducing the dissociation of Mule from its inhibitor ARF. Inhibition of Mule phosphorylation by silencing Syk prevents this, thereby inhibiting Mule E3 ligase activity and TNF-induced JNK activation and cell death. PMID: 26212014
    33. Study establishes P-SYK as a critical biomarker in AML that identifies tumors sensitive to SYK inhibition, identifies an at-risk patient population, and allows for the monitoring of target inhibition during treatment. PMID: 26315286
    34. LMPs are important pro-apoptotic regulators for Rb cells through reduction of SYK expression. PMID: 26404525
    35. Data suggest that immune complexes (ICs) of FCGR3A-SYK play critical role in activation of CD4+ T-cells and thus in modulation of adaptive immunity; such ICs are present in blood from patients with systemic lupus erythematosus. PMID: 26582197
    36. Syk activation is prominent in infiltrating myeloid cells in human rapidly progressive glomerulonephritis PMID: 26251216
    37. Results suggest that the activated Syk-mediated TRAF6 pathway leads to aberrant activation of B cells in SLE. PMID: 25432781
    38. this study shows that the treatment of cells with inducers of stress granule formation leads to the recruitment of Syk to these protein-RNA complexes PMID: 26429917
    39. The differential requirements of ZAP70 and SYK during thymic development. PMID: 26187144
    40. these data support the translation of approaches targeting Syk with fostamatinib to the clinic for patients with relapsed and possibly even newly diagnosed Waldenstrom macroglobulinemia PMID: 25748087
    41. Syk is constitutively associated with TLR4. PMID: 25896065
    42. The SYK -803 A>T genotypes TA and TT are independent risk factors for colorectal cancer development in Han Chinese in Southern China PMID: 25921550
    43. Data indicate that MINCLE receptor is able to mediate the response to trehalose-6,6-dimycolate (TDM) dependent on SYK kinase and CARD9 protein. PMID: 26202982
    44. find that both Syk and LMP2A have an effect on ITGbeta4 cell surface expression PMID: 25531330
    45. Demonstrate role for CBL in the control of AXL/SYK/LYN network mediating resistance to nilotinib treatment in chronic myeloid leukemia cells. PMID: 25965880
    46. SYK is downstream of CYR61 and contributes to CYR61-mediated mitoxantrone resistance. The CYR61-SYK pathway represents a potential target for reducing stroma-induced chemoresistance PMID: 25974135
    47. Results show that paclitaxel-resistant cells expressed higher level of phospho-SYK targeting tubulins and microtubule-associated proteins. PMID: 26096845
    48. An inflammatory Syk-ERK signaling axis requires DENV immune complexes, because DENV-2 in the presence of serotype-matched anti-DENV-2 mAb, but not anti-DENV-1 mAb, activates Syk, ERK, and IL-1beta secretion. PMID: 26032420
    49. PRT-060318 had a significant, albeit partial, inhibitory effect, thus suggesting that ROS induce NET formation in part via activation of Syk. PMID: 25277753
    50. The results reveal a Syk-dependent pathway of CpG-induced B cell stimulation, which is initiated at the plasma membrane and seems to be an upstream requirement for endosomal TLR9-driven B cell proliferation and differentiation. PMID: 25543269

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  • 亚细胞定位：
    Cell membrane. Cytoplasm, cytosol.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, SYK/ZAP-70 subfamily
  • 组织特异性：
    Widely expressed in hematopoietic cells (at protein level). Expressed in neutrophils (at protein level). Within the B-cell compartment, expressed from pro- and pre-B cells to plasma cells.
  • 数据库链接：

    HGNC: 11491

    OMIM: 600085

    KEGG: hsa:6850

    STRING: 9606.ENSP00000364898

    UniGene: Hs.371720