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  4. Phospho-TBC1D4 (T642) Antibody

Phospho-TBC1D4 (T642) Antibody

  • 货号：
    CSB-PA080083
  • 规格：
    ￥880
  • 图片：
    • Western blot analysis of L929 using p-TBC1D4 (T642) antibody.
  • 其他：

产品详情

  • Uniprot No.：
    O60343
  • 基因名：
    TBC1D4
  • 别名：
    Acrg embryonic lethality (mouse) minimal region ortholog antibody; Acrg embryonic lethality minimal region ortholog antibody; Acrg embryonic lethality mouse minimal region ortholog antibody; Akt substrate of 160 kDa antibody; AS 160 antibody; AS160 antibody; BUB2 antibody; CDC16 antibody; KIAA0603 antibody; NIDDM5 antibody; TBC (Tre 2 BUB2 CDC16) domain containing protein antibody; TBC Tre 2 BUB2 CDC16 domain containing protein antibody; TBC1 D4 antibody; TBC1 domain family member 4 antibody; Tbc1d4 antibody; TBCD4_HUMAN antibody; Tre-2 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from Human TBC1D4 around the phosphorylation site of T642.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, IF, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:100-1:300
    IF 1:200-1:1000
    ELISA 1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
  • 基因功能参考文献：
    1. C-terminal region (CTR) region is largely alpha-helical and mediates TBC1D4 RabGAP dimerization PMID: 28545963
    2. Disruption of TBC1D4 is common among North American Inuit, resulting in exclusively elevated postprandial glucose. This leads to underdiagnosis of type 2 diabetes, unless an OGTT is performed. PMID: 27561922
    3. AS160 regulates glucose-independent eukaryotic cell proliferation through p21-dependent control of the cell cycle. PMID: 27152871
    4. AS160 and TBC1D1 phosphorylations were evident 30 min after exercise. PMID: 24876356
    5. homozygous carriers of a nonsense p.Arg684Ter variant have markedly higher concentrations of plasma glucose and serum insulin 2 hours after an oral glucose load compared with individuals with other genotypes PMID: 25043022
    6. Findings suggest that a dampening of insulin-induced phosphorylation of AS160 on specific sites in skeletal muscle contributes to the insulin resistance evident in a sedentary aging population PMID: 23801578
    7. insulin resistance in muscles from healthy individuals is associated with suppression of site-specific phosphorylation of AS160 PMID: 22028408
    8. AS160 interacts with the large cytoplasmic NP domain of the alpha-subunit of the Na(+),K(+)-ATPase. AMP-stimulated protein kinase (AMPK) and AS160 participate in a common pathway to modulate the cell surface expression of the Na(+),K(+)-ATPase. PMID: 20943949
    9. Crystal structures of human TBC1D1 and TBC1D4 (AS160) RabGTPase-activating protein (RabGAP) domains reveal critical elements for GLUT4 translocation. PMID: 21454505
    10. AS160 phosphotyrosine-binding domain constructs inhibit insulin-stimulated GLUT4 vesicle fusion with the plasma membrane PMID: 21454690
    11. Impaired insulin-induced site-specific TBC1D4 phosphorylation may contribute to skeletal muscle insulin resistance in type 2 diabetes. PMID: 20938636
    12. results show AS160 phosphorylation level is frequently increased in breast cancer; results implicate a possible role of AS160 in breast tumorigenesis and suggest that p-AS160 might be useful as a marker and a potential novel treatment target PMID: 20574165
    13. WNK1 promotes cell surface expression of glucose transporter GLUT1 by regulating a Tre-2/USP6-BUB2-Cdc16 domain family member 4 (TBC1D4)-Rab8A complex PMID: 20937822
    14. S711 is a novel TBC1D4 phosphorylation site regulated by AMPK in skeletal muscle. PMID: 19923418
    15. KIAA0603 is likely to be a Rab GAP that participates in the regulation of activated T cells in atopic dermatitis PMID: 15304337
    16. In this study, we determined the expression of and in vivo insulin action on AS160 in skeletal muscle of normal and type 2 diabetic patients. PMID: 15919790
    17. results indicate that AS160 is a Rab GAP, and suggest novel Rabs that may participate in GLUT4 translocation PMID: 15971998
    18. AS160 is phosphorylated in a time-dependent manner during moderate-intensity exercise. PMID: 17077344
    19. Regulation of AS160 and interaction with 14-3-3 in skeletal muscle are influenced by resistance exercise and insulin but do not fully explain the effect of resistance exercise on whole-body insulin action. PMID: 17369524
    20. Effects of endurance exercise training on insulin signaling and AS160 in human skeletal muscle PMID: 17513702
    21. AS160 is a common target of insulin, IGF-1, EGF, PMA and AICAR, these stimuli induce distinctive patterns of phosphorylation and 14-3-3 binding, mediated by at least four protein kinases. PMID: 17617058
    22. Impaired insulin signaling through Akt and AS160 in part explains insulin resistance at the molecular level in skeletal muscle in polycystic ovary syndrome. PMID: 17977950
    23. AS160, previously recognized as a key player in insulin signaling in skeletal muscle and adipose tissue, is also a major effector of protein kinase B/Akt signaling in the beta-cell. PMID: 18276765
    24. The transcript variant 2 of AS160 in contrast to full-length AS160 is a novel regulator of glucose transport that positively influences glucose-uptake rates. PMID: 18771725
    25. muscle TBC1D4 phosphorylation across the leg is increased during recovery following resistance exercise PMID: 18845784
    26. Specific phosphorylation of TBC1D4 in human skeletal muscle in response to physiological exercise-induced hyperinsulinemia. PMID: 19252894
    27. Prematurely truncated TBC1D4 protein tended to increase basal cell membrane GLUT4 levels (P = 0.053) and significantly reduced insulin-stimulated GLUT4 cell membrane translocation (P < 0.05). PMID: 19470471

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  • 相关疾病：
    Diabetes mellitus, non-insulin-dependent, 5 (NIDDM5)
  • 亚细胞定位：
    Cytoplasm. Note=Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
  • 组织特异性：
    Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland,
  • 数据库链接：

    HGNC: 19165

    OMIM: 612465

    KEGG: hsa:9882

    STRING: 9606.ENSP00000366863

    UniGene: Hs.210891