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  4. Phospho-WASF1 (Tyr125) Antibody

Phospho-WASF1 (Tyr125) Antibody

  • 货号：
    CSB-PA565197
  • 规格：
    ￥2454
  • 图片：
    • Western blot analysis of extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins), using WAVE1 (Phospho-Tyr125) antibody. The lane on the right is treated with the synthesized peptide.
    • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using WAVE1 (Phospho-Tyr125) antibody. The picture on the right is treated with the synthesized peptide.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) WASF1 Polyclonal antibody
  • Uniprot No.：
    Q92558
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Peptide sequence around phosphorylation site of tyrosine 125 (E-T-Y(p)-D-V) derived from Human WAVE1.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes. Also involved in the regulation of mitochondrial dynamics.
  • 基因功能参考文献：
    1. Results from a study on gene expression variability markers in early-stage human embryos shows that WASF1 is a putative expression variability marker for the 3-day, 8-cell embryo stage. PMID: 26288249
    2. Results suggested that WAVE1 is a critical pro-autophagic protein capable of enhancing cell survival and regulating chemoresistance in leukemia cells potentially through the Beclin1/Bcl-2 and Beclin1/PI3K- complex-dependent pathways. PMID: 27035872
    3. Results implicate a contributory role of WAVE1 and -3 to the metastatic phenotype of PC-3 cells through their interaction with the ARP2/3 complex. PMID: 26977009
    4. we propose that WASF1 status defines a subtype of androgen deprivation therapy -resistant prostate cancer patients PMID: 25906751
    5. a role for ARF6 in linking EGF-receptor signaling to Rac1 recruitment and activation at the plasma membrane to promote breast cancer cell directed migration PMID: 25799492
    6. A decrease in amounts of WASF1 mRNA was also observed in human Alzheimer's disease brains, suggesting clinical relevance of the negative feedback circuit involved in homeostatic regulation of Abeta production PMID: 26280122
    7. D620N mutation in VPS35 restricts WASH complex recruitment to endosomes, and reveals a novel role for the WASH complex in autophagosome formation. PMID: 24819384
    8. WAVE1 has unique activities independent of Arp2/3 complex that can govern both the growth rates and architectures of actin filament networks. Elongation inhibitory effects of WAVE1 were mapped to its WH2 ("V") domain. PMID: 25473116
    9. The WAVE complex is the main activator of the Arp2/3 complex for actin filament nucleation and assembly in the lamellipodia of moving cells. PMID: 25355952
    10. WAVE1 might promote the proliferative and invasive malignant behaviors through the activation of the PI3K/AKT and p38MAPK signaling pathways in epithelial ovarian cancer. PMID: 23680521
    11. Scar/WAVE regulatory complex and N-WASP play opposing roles in 3D epithelial cell migration PMID: 23273897
    12. mRNAs encoding structural and regulatory components of the WAVE complex are localized to the leading edge of the cell, suggesting that localized protein synthesis plays a pivotal role in controlling cell spreading and migration. PMID: 23452202
    13. Study finds that WAVE1 overexpression is associated with an unfavorable prognosis. WAVE1 is an independent prognostic factor for EOC, which suggests that it is a novel and crucial predictor for EOC metastasis. PMID: 22721732
    14. mDia1 and WAVE2 are important Src homology 3 domain partners of IRSp53 in forming filopodia. PMID: 22179776
    15. Arf GTPases may be central components in WAVE signalling, acting directly, alongside Rac1. PMID: 21844371
    16. WAVE1 might be involved in the migration and invasion of K562 cells through regulation of the expression level of MMP-2. PMID: 19731823
    17. Higher levels of WAVE1 in the bone marrow indicate an unfavorable prognosis in children with AML. PMID: 20426950
    18. Dock3 induces axonal outgrowth by stimulating membrane recruitment of the WAVE complex PMID: 20368433
    19. WAVE1 regulates Bcl-2 localization and phosphorylation in leukemia cells. PMID: 19890377
    20. Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck: we propose that Rac1 and Nck cause dissociation of the WAVE1 complex, which releases active WAVE1-HSPC300 and leads to actin nucleation. PMID: 12181570
    21. WAVE1 may act as a scaffold to recruit the NADPH oxidase to a complex involved with both cytoskeletal regulation and downstream JNK activation PMID: 12855698
    22. the 3 WAVE isoforms exhibit common and distinct features and may potentially be involved in the regulation of actin cytoskeleton in platelets PMID: 15280206
    23. Dictyostelium discoideum has been used to remove genetically SCAR complex members to ascertain their specific roles. PMID: 15506982
    24. WAVE-1 expression was associated with megakaryocytic differentiation; WAVE-1 and WAVE-2 moved from a detergent-soluble cytosolic fraction to insoluble cytoskeleton fraction after platelet aggregation PMID: 15670045
    25. NESH (Abi-3), like Abi-1 and Abi-2, is a component of the Abi/WAVE complex, but likely plays a different role in the regulation of c-Abl. PMID: 17101133
    26. the Hem-1/Nap1 component of the Scar/WAVE complex localizes to propagating waves that appear to organize the leading edge of a motile neutrophil PMID: 17696648
    27. WAVE1 is critical for formation of oligodendrocyte lamellae and myelin sheaths--REVIEW PMID: 17901257
    28. WAVE1 is involved in multi-drug resistance through regulation the level of mdrl and Bcl-2. PMID: 17939402
    29. WAVE1 dephosphorylation and activation are likely associated with mitochondrial redistribution and dendritic spine morphogenesis. PMID: 18287015
    30. Results suggest that WAVE and the Arp2/3 complex jointly orchestrate different types of actin-based plasma membrane protrusions by promoting ruffling and inhibiting mDia2-induced filopodia. PMID: 18516090
    31. The WAVE1 expression increased in children with ALL. WAVE1 may be related to the development of ALL and may be severed as a marker for the evaluation of the severity of ALL in children. PMID: 18947485
    32. WAVE1 and p22phox expression in PBMCs increased and was associated with the disease course in children with acute lymphocytic leukemia (ALL). PMID: 19222940
    33. WAVE accumulation may be involved in Abeta/amyloid precursor protein mediated-tangle modification PMID: 19497998

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell junction, synapse. Cell junction, focal adhesion.
  • 蛋白家族：
    SCAR/WAVE family
  • 组织特异性：
    Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.
  • 数据库链接：

    HGNC: 12732

    OMIM: 605035

    KEGG: hsa:8936

    STRING: 9606.ENSP00000352425

    UniGene: Hs.75850