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RBM5 Antibody

  • 货号：
    CSB-PA239401
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from HUVEC cells, using RBM5 antibody.
    • Immunohistochemistry analysis of paraffin-embedded human brain tissue using RBM5 antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) RBM5 Polyclonal antibody
  • Uniprot No.：
    P52756
  • 基因名：
    RBM5
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from internal of Human RBM5.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate apoptosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.
  • 基因功能参考文献：
    1. RNA binding motif protein 10 (RBM10) negatively regulates its own mRNA and protein expression and that of RNA binding motif protein 5 (RBM5) by promoting alternative splicing-coupled nonsense-mediated mRNA decay (AS-NMD). PMID: 28586478
    2. results provide evidence that RBM10 expression, in RBM5-null tumors, may contribute to tumor growth and metastasis. Measurement of both RBM10 and RBM5 expression in clinical samples may therefore hold prognostic and/or potentially predictive value PMID: 28662214
    3. the mechanism of action of RBM5-AS1 in the WNT pathway via physical interactions with beta-catenin, helping organize transcriptional complexes that sustain colon cancer-initiating cells function. PMID: 27520449
    4. Low RBM5 expression was significantly associated with gliomas. PMID: 28061901
    5. RNA-binding motif 5 silencing reduced the messenger RNA and protein expression of the p53 target gene p21. Our results suggest that RNA-binding motif 5 downregulation is involved in gastric cancer progression and that RNA-binding motif 5 behaves as a tumor suppressor gene in gastric cancer PMID: 28347247
    6. Results show that RBM5 is downregulated in smoke induced tumor; its overexpression inhibits tumor growth through cell cycle arrest and induction of apoptosis suggesting the importance of RBM5 in the pathogenesis of smoking-related cancer. PMID: 26782095
    7. ICG-001 had no apparent effect on the RBM5 levels. PMID: 25738917
    8. RBM5 knockdown in human neuronal cells decreases caspase activation by staurosporine. PMID: 25586139
    9. Loss of RBM5 expression is associated with lung adenocarcinoma. PMID: 23721095
    10. expression of RBM5 protein was significantly decreased in cancerous prostatic tissues compared to the normal tissues PMID: 23158838
    11. RBM5 can inhibit the growth of lung cancer cells and induce apoptosis both in vitro and in vivo. PMID: 22866867
    12. Expression of RBM5 mRNA and protein was negatively correlated with expression of EGFR and KRAS mRNA and protein in NSCLC tissues. PMID: 22537942
    13. RBM5 promotes exon 4 skipping of AID pre-mRNA by competing with the binding of U2AF65 to the polypyrimidine tract. PMID: 23017209
    14. RBM5 may serve as a biomarker with the ability to predict a response to cisplatin PMID: 22609235
    15. The results suggest that RBM5 expression is not directly regulated by EGFR in non-smoker related lung adenocarinomas. PMID: 22882865
    16. NMR data analysis of RBM5 RRM2 reveals several features of protein-RNA interfaces. The most striking observation is the presence of two preferred target RNA sequences, the sequences [5'-(CUCUUC)-3'] and [5'-(GAGAAG)-3']. PMID: 22839758
    17. Data suggest that G-patch domain of RBM5 is indispensable for its ability to interact with DHX15; RBM5 stimulates helicase activity of DHX15 in a G-patch domain-dependent manner. PMID: 22569250
    18. alters expression of genes involved in metastasis PMID: 20338664
    19. p53 transactivation is involved in the antiproliferative activity of the putative tumor suppressor RBM5. PMID: 20309933
    20. Over-expression of the splice variant LUCA15 shortened the cell cycle and inhibited CD95-mediated apoptosis in CEM-C7 T-cells. PMID: 12581154
    21. LUCA-15/RMB5 sensitises Jurkat cells to apoptosis initiated by anti-Fas antibody, TNF-alpha, or TRAIL. PMID: 15192330
    22. Results provide the first evidence of an apoptotic modulatory role for LUCA-15 in MCF-7 breast carcinoma cell line, which is a non-T cell line. PMID: 15338470
    23. Results suggest that LUCA-15 plays a central role in regulating cell fate consistent with its tumor suppressor activity. PMID: 16546166
    24. H37 was transfected into A549 non-small cell lung cancer cells. A549/H37 cells show significant growth inhibition compared with the vector controls by in vitro and in vivo cell proliferation assays. PMID: 16585163
    25. Results suggest that reversible phosphorylation of RBM5 is a mechanism capable of regulating RBM5 participation in modulating apoptosis, and perhaps tumour suppression. PMID: 16927403
    26. analysis of Hedgehog modulator properties after functional coupling of Smoothened to G15 PMID: 16945339
    27. specific allele types at C1138T and C2185T single nucleotide polymorphisms positions are correlated with different histological subtypes of non-small cell lung cancer PMID: 17606309
    28. In breast tumour tissue, chimeric expression was associated with elevated levels of RBM5 mRNA, and increased tumour size. PMID: 17908320
    29. suggest some of the possible, further- alternative means of the H37/RMB5 gene expression loss in tumor, including defects in transcription and post-transcriptional/translational modifications as well as mechanisms related to haploinsufficiency PMID: 18038152
    30. RBM5 binds to casp-2 pre-mRNA at a U/C-rich sequence immediately upstream of the previously identified In100 splicing repressor element PMID: 18840686
    31. RBM5 inhibits the transition between prespliceosomal complexes assembled around exon 6 to mature spliceosomes assembled on the flanking introns and promotes sequence-specific pairing of the distal splice sites. PMID: 18851835
    32. LUST is a novel, functional, non-coding RNA that plays a role in determining the apoptotic fate of a cell by regulating the expression of RBM5 splice variants. PMID: 19559772

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    RBM5/RBM10 family
  • 组织特异性：
    Isoform 5 is widely expressed in normal tissues and is expressed at increased levels in T-leukemic cell lines.
  • 数据库链接：

    HGNC: 9902

    OMIM: 606884

    KEGG: hsa:10181

    STRING: 9606.ENSP00000343054

    UniGene: Hs.439480