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RHAG Antibody

  • 货号：
    CSB-PA005318
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of K562 cells using CD241 Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    Q02094
  • 基因名：
    RHAG
  • 别名：
    RHAG antibody; RH50 antibody; Ammonium transporter Rh type A antibody; Erythrocyte membrane glycoprotein Rh50 antibody; Erythrocyte plasma membrane 50 kDa glycoprotein antibody; Rh50A antibody; Rhesus blood group family type A glycoprotein antibody; Rh family type A glycoprotein antibody; Rh type A glycoprotein antibody; Rhesus blood group-associated ammonia channel antibody; Rhesus blood group-associated glycoprotein antibody; CD antigen CD241 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from the N-terminal region of Human CD241.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    ELISA 1:20000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Associated with rhesus blood group antigen expression. May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane. Involved in ammonia transport across the erythrocyte membrane. Seems to act in monovalent cation transport.
  • 基因功能参考文献：
    1. A novel nucleotide deletion in RHAG allele identified in a Chinese Rhnull individual. PMID: 29266289
    2. Case Report: complex RHAG genotype including a novel de novo mutation associated with overhydrated stomatocytosis. PMID: 29559519
    3. These results indicated that the 540C>A nonsense mutation in RHAG gene caused the regulator type of Rhnull phenotype in a Chinese individual. PMID: 28063760
    4. novel allele in a Brazilian pregnant woman encoding the Rhnull phenotype due to a change in RHAG exon2 c.310C>T, which leads to a premature stop codon (Gln104Stop). PMID: 26175207
    5. We characterized ammonia and ammonium (NH3/NH4 (+)) transport by the rhesus-associated (Rh) glycoproteins RhAG, Rhbg, and Rhcg expressed in Xenopus oocytes. We used ion-selective microelectrodes and two-electrode voltage clamp to measure changes in intracellular pH, surface pH, and whole cell currents induced by NH3/NH4 (+) and methyl amine/ammonium (MA/MA(+)). PMID: 26354748
    6. A new Rh null allele (RHAG*01N.13) of the regulator type found in a consanguineous French-speaking quebecers'family. PMID: 25296744
    7. RhAG, RhBG and RhCG-exhibit significant permeability to NH3 and show for the first time that RhBG and RhCG can conduct CO2. PMID: 24077989
    8. Data from differentiating cultured erythroid precursor cells suggest that RhAG knockdown abolishes Rh blood group expression (RhoD [ras homolog family member D]; ICAM4 [intercellular adhesion molecule 4]; CD47 Rh-related antigen) in erythroid cells. PMID: 23417980
    9. Substitution of GPB with Gp.Mur significantly reduced the expression of Rh antigen and RhAG on the Mi.III(+/+) erythrocyte membrane PMID: 21883272
    10. Results provide new insights into the functional impact of the Phe65Ser mutation in RhAG. PMID: 22012326
    11. that the 672C>A missense mutation in the RHAG gene could result in Rh(null) of the regulator type, and the single-amino-acid change (Ser to Arg) might be critical for assembly of the Rh antigen complex within the membrane PMID: 21682734
    12. The results provide new insight into RhAG stomatocytosis mutant F65S as a combined loss-of-function/gain-of-function mutation for methylammonium/methylammonium+ transport PMID: 21849667
    13. identification as a mammalian ammonium transporter PMID: 11861637
    14. Cell-surface expression of RhD blood group polypeptide is posttranscriptionally regulated by the RhAG glycoprotein. PMID: 12130520
    15. interactions of CD47 and RhAG and the Rh proteins with one another and with the cytoskeleton of intact erythrocytes PMID: 12393442
    16. RhAG functions as a NH(4)(+)/H(+) exchanger; ammonium transport is coupled to the H(+) gradient PMID: 14966114
    17. RhAG facilitates CH(3)NH(2)/NH(3) movement across the RBC membrane and represents a potential example of a gas channel in mammalian cells. PMID: 15572441
    18. The combination of these polymorphisms could not be found in any control individuals, suggesting that they might be involved in genetic predisposition to migraine in this family. PMID: 16378686
    19. RhAG-mediated transport is an electroneutral process that is driven by the NH4+ concentration and the transmembrane H+ gradient, effectively exchanging NH4+ for H+ in a process that results in transport of net NH3. PMID: 16563829
    20. RhAG expression enhanced the ammonium-induced initial alkalinization (related to NH3 influx)& secondary acidification (related to NH4+ influx). Sub-millimolar NH4+ concentrations induced inward currents in voltage-clamped RhAG-expressing cells. PMID: 16564724
    21. Review. RhAG plays a major role in the NH3 conductance of erythrocytes, but probably not in CO2 transport. PMID: 16574458
    22. Rh protein, presumably the Rh-associated glycoprotein RhAG, possesses a gas channel that allows passage of CO2 in addition to NH3 PMID: 17712059
    23. Reduced amounts of Rh-associated glycoprotein is associated with overhydrated hereditary stomatocytosis. PMID: 18931342
    24. gas channels exhibit selectivity for CO(2) vs. NH(3) permeability, demonstrating the sequence AQP4 congruent with AQP5 > AQP1 > AmtB > RhAG. PMID: 19273840

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  • 相关疾病：
    Regulator type Rh-null hemolytic anemia (RHN); Overhydrated hereditary stomatocytosis (OHST)
  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ammonium transporter (TC 2.A.49) family, Rh subfamily
  • 组织特异性：
    Erythrocytes.
  • 数据库链接：

    HGNC: 10006

    OMIM: 180297

    KEGG: hsa:6005

    STRING: 9606.ENSP00000360217

    UniGene: Hs.120950