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RHBDD3 Antibody

  • 货号：
    CSB-PA27742A0Rb
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: PC-3 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate
      All lanes: RHBDD3 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 41 kDa
      Observed band size: 41 kDa
    • IHC image of CSB-PA27742A0Rb diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of U251 cells with CSB-PA27742A0Rb at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) RHBDD3 Polyclonal antibody
  • Uniprot No.：
    Q9Y3P4
  • 基因名：
    RHBDD3
  • 别名：
    C22orf3 antibody; HS984G1A antibody; Pituitary tumor apoptosis antibody; PTAG antibody; RHBD3_HUMAN antibody; RHBDD3 antibody; Rhomboid domain containing 3 antibody; Rhomboid domain-containing protein 3 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Peptide sequence from Human Rhomboid domain-containing protein 3 protein (304-323AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，RHBDD3 Antibody (CSB-PA27742A0Rb)，的标记方式是Non-conjugated。对于RHBDD3 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA27742B0Rb RHBDD3 Antibody, HRP conjugated ELISA
    FITC CSB-PA27742C0Rb RHBDD3 Antibody, FITC conjugated
    Biotin CSB-PA27742D0Rb RHBDD3 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 基因功能参考文献：
    1. RHBDD3 is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
    2. isolation of a novel differentially methylated chromosome 22 CpG island-associated gene PTAG PMID: 15105437
    3. Loss of PTAG contributes to a blunted apoptotic response and is likely to predispose cells toward malignant transformation and resistance to chemotherapeutic interventions. PMID: 17117413
    4. the EWSR1 promoter functions in a bidirectional manner, thereby regulating also RHBDD3 PMID: 19212622
  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 数据库链接：

    HGNC: 1308

    KEGG: hsa:25807

    STRING: 9606.ENSP00000216085

    UniGene: Hs.106730