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  4. RNF40 Antibody

RNF40 Antibody

  • 货号：
    CSB-PA777302
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA777302(RNF40 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA777302(RNF40 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
    • Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Hela cells, 231 cells, A172 cells, Primary antibody: CSB-PA777302(RNF40 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
  • 其他：

产品详情

  • Uniprot No.：
    O75150
  • 基因名：
    RNF40
  • 别名：
    95 kDa retinoblastoma associated protein antibody; 95 kDa retinoblastoma protein binding protein antibody; 95 kDa retinoblastoma-associated protein antibody; BRE 1B antibody; BRE1 B antibody; BRE1 E3 ubiquitin ligase homolog B antibody; BRE1-B antibody; BRE1B antibody; BRE1B_HUMAN antibody; DKFZp686K191 antibody; E3 ubiquitin protein ligase BRE1B antibody; E3 ubiquitin-protein ligase BRE1B antibody; KIAA0661 antibody; MGC13051 antibody; Rb associated protein antibody; RBP 95 antibody; RBP95 antibody; RING finger protein 40 antibody; Ring finger protein 40 E3 ubiquitin protein ligase antibody; RNF 40 antibody; Rnf40 antibody; STARING antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Fusion protein of Human RNF40
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    WB 1:200-1:1000
    IHC 1:25-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.
  • 基因功能参考文献：
    1. the RNF20/40 complex, a major ubiquitin ligase catalysing histone H2B monoubiquitination, interacts with the motor protein Eg5 during mitosis and participates in spindle assembly. PMID: 27557628
    2. The s also show that the RING domains of RNF20 and RNF40 can form a stable heterodimer that is active. PMID: 27569044
    3. Manipulation of key H2Bub1 E3 ubiquitin ligases, RNF20, RNF40 and BRCA1, in ovarian cancer cell line models modulated H2Bub1 levels, indicative of the role of these RING finger ligases in monoubiquitination of H2Bub1 in vitro PMID: 27798111
    4. We show that Bre1 (human BRE1A/B (RNF20/40) and mouse Bre1a/b (Rnf20/40)) acts as an important suppressor of chromosomal instability PMID: 22354749
    5. our results suggest that RNF20 and RNF40, either via ubiquitylation of H2B or other targets, are coupled to the proliferation of prostate cancer cells. PMID: 22155569
    6. RNF40 cooperates with SUPT16H to induce dynamic changes in chromatin structure during DNA double-strand break repair. PMID: 22031019
    7. the observed defects in the radiation response of Bre1a/b-deficient cells PMID: 20738173
    8. Formation of trimeric complex UbcH6 and RNF20/40 with PAF stimulates histone 2B monoubiquitination activity in vitro PMID: 16307923
    9. Functional Analysis of the rat counterpart PMID: 12121982

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    BRE1 family
  • 组织特异性：
    Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine.
  • 数据库链接：

    HGNC: 16867

    OMIM: 607700

    KEGG: hsa:9810

    STRING: 9606.ENSP00000325677

    UniGene: Hs.65238