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RPL5 Antibody

  • 货号：
    CSB-PA276101
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from HepG2 cells and Jurkat cells, using RPL5 antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) RPL5 Polyclonal antibody
  • Uniprot No.：
    P46777
  • 基因名：
    RPL5
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from internal of Human RPL5.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53.
  • 基因功能参考文献：
    1. RPL5 remains an interesting candidate in multiple myelom(MM )because it is deleted in 20-40% of MM cases PMID: 28428269
    2. Whole exome sequencing in the differential diagnosis of Diamond-Blackfan anemia: Clinical and molecular study of three patients with novel RPL5 and mosaic RPS19 mutations PMID: 28376382
    3. Low RPL5 expression is associated with cancer. PMID: 28147343
    4. Ribosomal proteins L11 and L5 activate TAp73 by overcoming MDM2 inhibition. PMID: 25301064
    5. RPL5 mutation is associated with Diamond Blackfan Anemia. PMID: 25132370
    6. Findings uncover a mechanism by which RPL5 and RPL11 can co-operatively suppress c-Myc expression, allowing a tightly controlled ribosome biogenesis in cells. PMID: 24141778
    7. Unlike other tumor suppressors, RPL5 and RPL11 play essential roles in normal cell proliferation. PMID: 24061479
    8. High frequency of RPL5 gene deletion is associated with Italian Diamond-Blackfan anemia. PMID: 22689679
    9. Oncogenic splicing factor SRSF1 stabilizes the tumor suppressor protein p53 via RPL5, inducing cell senescence. PMID: 23478443
    10. Mutations affect the ribosomal proteins RPL5 and RPL10 in 12 of 122 (9.8%) pediatric T-cell acute lymphoblastic leukemias. PMID: 23263491
    11. disrupted nucleoli may provide a platform for L5- and L11-dependent p53 activation, implying a role for the nucleolus in p53 activation by ribosomal biogenesis stress PMID: 23169665
    12. Data show 1 proband with an RPL5 deletion, 1 patient with an RPL35A deletion, 3 with RPS17 deletions, and 1 with an RPS19 deletion. PMID: 22262766
    13. Data show that all patients with RPS19 and RPL5 mutations had physical abnormalities. PMID: 20378560
    14. An analysis and fine mapping of GFI-EVI5-RPL5-FAM69A locus, genotyping eight Tag-single nucleotide polymorphisms in 732 multiple sclerosis patients and 974 controls from Spain, was performed. PMID: 20087403
    15. Knockdown of L29 or L30 enhanced the interaction of MDM2 with L11 and L5 and markedly inhibited MDM2-mediated p53 ubiquitination, suggesting that direct perturbation of 60 S ribosomal biogenesis activates p53 via L11- and L5-mediated MDM2 suppression. PMID: 20554519
    16. The study reports a high frequency of RPL5 (9.3%) and RPL11 (9.3%) mutations in a Diamond-Blackfan anemia cohort. PMID: 19773262
    17. the presence of multiple NLSs in ribosomal protein L5 appears to allow for efficient nuclear transport via utilisation of multiple, mechanistically different import pathways. PMID: 11824785
    18. the MDM2-L5-L11-L23 complex functions to inhibit MDM2-mediated p53 ubiquitination and thus activates p53 PMID: 15308643
    19. interaction of NVL2 with L5 is ATP-dependent and likely contributes to the nucleolar translocation of NVL2 PMID: 15469983
    20. Cancer-associated missense mutations targeting MDM2's central zinc finger disrupt the interaction of MDM2 with L5 and L11. PMID: 17116689
    21. 5-FU treatment triggers a ribosomal stress response so that ribosomal proteins L5, L11, and L23 are released from ribosome to activate p53 by ablating the MDM2-p53 feedback circuit PMID: 17242401
    22. L11 cooperates with L5, resulting in a robust inhibition of the E3 activity of MDM2, and a stabilization and activation of p53 approaching that achieved by p14(ARF). PMID: 18560357
    23. Susceptibility gene for multiple sclerosis in Australians. PMID: 18650830
    24. RPL5 mutations are associated with cleft palate and abnormal thumbs in Diamond-Blackfan anemia patients. PMID: 19061985
    25. Mutations in RPL5 were identified in eight patients from 6 out of 28 families (21.4%). PMID: 19191325

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  • 相关疾病：
    Diamond-Blackfan anemia 6 (DBA6)
  • 亚细胞定位：
    Cytoplasm. Nucleus, nucleolus.
  • 蛋白家族：
    Universal ribosomal protein uL18 family
  • 数据库链接：

    HGNC: 10360

    OMIM: 603634

    KEGG: hsa:6125

    STRING: 9606.ENSP00000359345

    UniGene: Hs.532359