RPOD Antibody

1. Counter-intuitively, intermolecular epistasis can alleviate the constraints of individual components, thereby increasing phenotypic variation that selection could act on and facilitating adaptive evolution. PMID: 29130883
2. In contrast to NusG, which freely binds to RNA polymerase, RfaH exists in a structurally distinct autoinhibitory state in which the RNA polymerase-binding site is buried at the interface between two RfaH domains. PMID: 28605514
3. Here the s establish that the primary sigma factor in Escherichia coli, sigma(70), can function as an elongation factor in vivo by loading directly onto the transcription elongation complex in trans. PMID: 26371553
4. These data suggested that template strand single-stranded DNA competes with lipiarmycin for binding to RNA polymerase and that sigma(70) regions 1.2 and 3.2 attenuate lipiarmycin action by promoting DNA duplex opening. PMID: 26724534
5. The sigma70-subunit of E. coli RNA polymerase (a small protein, being a part of RNA holoenzyme, and responsible for initiation of transcription of constitutive genes) is modeled at different ionic strengths PMID: 26841497
6. Data suggest that RNAP sigma 70 interactions with DNA immediately downstream of the transcription bubble are particularly important for initiation of transcription. PMID: 25311862
7. s conclude that bacteriophage interruption of mlrA and RpoS mutations provide major obstacles limiting curli expression and biofilm formation in most serotype O157 : H7 strains. PMID: 23744902
8. where at least three regions of the 6S RNA must interact with Esigma(70) in a cooperative manner so as to ensure effective pRNA-dependent release. PMID: 24681966
9. The study presents the initial steps of the 6S RNA - RNA polymerase complex decay. PMID: 23667906
10. X-ray crystal structure of Escherichia coli RNA polymerase sigma70 holoenzyme PMID: 23389035
11. Deliberately slowing the intrinsic 6S RNA release rate by nucleotide feeding experiments reveals that sigma70 ejection occurs abruptly once a pRNA length of 9 nucleotides is reached. PMID: 23118417
12. study found that although Rob makes fewer interactions with sigma(70) R4 than SoxS, the 2 proteins make the same position-dependent interactions; Rob occludes sigma(70) R4 from binding the -35 hexamer, just as does SoxS; thus, the C-terminal domain does not substantially alter the way Rob interacts with sigma(70) R4 at class II promoters PMID: 22465792
13. Data indicate that region 1.1 and, to a lesser extent, region 1.2 of sigma(70) subunit determine higher promoter complex stability of RNA polymerase (RNAP). PMID: 22605342
14. changes in the structure of region 2 of the sigma(70) subunit have stronger effects on transcription pausing than on promoter recognition, likely by weakening the interactions of the sigma subunit with the core RNAP during transcription elongation. PMID: 22098235
15. The strength of the -15 element in sigma(70) promoter inversely correlates with the strengths of the -35 element and -10 element. PMID: 21908667
16. sigma70 drives yjbEFGH transcription and it also acts to repress its induction. PMID: 21396442
17. Mutation of sigma(70) residue R451, which is highly conserved, results in reduced growth rate, consistent with a central role in promoter recognition. PMID: 21398630
18. His180 substitutions influence several steps of transcription initiation, including core binding, promoter DNA recognition and open complex formation. PMID: 21451247
19. Genetic evidence for a novel interaction between transcriptional activator soxs and region 4 of the sigma(70) subunit of RNA polymerase at class ii SoxS-dependent promoters in Escherichia coli PMID: 21195716
20. SoxS is now the first example of an E. coli transcriptional activator that uses a single positive control surface to make specific protein-protein contacts with two different subunits of RNAP. PMID: 20595001
21. Phenotypically, cells overproducing sigma(70) favours growth and reproduction at the expense of motility and damage protection. PMID: 19574956
22. in vivo association of sigma(70) and NusA with elongating RNA polymerase is regulated by growth conditions PMID: 15596728
23. analysis of promoter DNA melting by Thermus aquaticus and Escherichia coli RNA polymerases PMID: 15731103
24. amino acid substitutions in region 4 of sigma(70) that have specific effects on the sigma(70) region 4/beta-flap RNA polymerase interaction, either weakening or strengthening it PMID: 15761057
25. Establishment of downstream contacts leads to remodeling of upstream interactions between sigma70 and the -10 promoter element that might facilitate promoter escape and sigma release PMID: 15978618
26. the recognition of the -35 region involves similar amino acid residues in regions 4.2 of E. coli sigma32 and sigma70 PMID: 16166539
27. the core of promoter melting activity of RNA polymerase is localized to a very small subset of all promoter-polymerase contacts PMID: 16169843
28. an important role of sigma region 3.2 in the binding of initiating substrates in RNA polymerase active center and in the process of promoter clearance PMID: 16690607
29. Sigma region 1.2 acts as a core RNA polymerase-dependent allosteric switch that modulates non-template DNA strand recognition by sigma region 2 during transcription initiation and elongation. PMID: 17268549
30. sigma(70) NCR/beta' interaction facilitates promoter escape and hinders early elongation pausing, in contrast to the sigma(2)/beta' coiled-coil interaction, which has opposite effects. PMID: 17332752
31. experiments support a model in which the role of Rsd is primarily to sequester sigma(70), thereby increasing the levels of RNA polymerase containing the alternative sigma(38) factor PMID: 17351046
32. The 2.6 A-resolution X-ray crystal structure of Rsd bound to sigma(70) domain 4 (sigma(70)(4)), the primary determinant for Rsd binding within sigma(70), was determined. PMID: 17681541
33. the T429 of Sigma 70 acts as a competitor for the hydrogen bonding that stabilizes the highly conserved - 11A-T base pairs of the promoter DNA, thus facilitating initiation of strand separation at this particular position in the - 10 region. PMID: 18155246
34. The tyrosine at residue 430 in region 2.3 of sigma(70) was shown to be involved in quenching the fluorescence of a 2-AP substituted at -11, presumably through a stacking interaction. PMID: 18976666
35. Residues in sigma70 region 4.2, the C-terminus of the second helix, contribute significantly to 6S RNA binding but not to binding of a minimal promoter. PMID: 19538447

显示更多

收起更多

KEGG: ecj:JW3039

STRING: 316385.ECDH10B_3242