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  4. RPS6KA1 (Ab-352) Antibody

RPS6KA1 (Ab-352) Antibody

  • 货号：
    CSB-PA906196
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from 293 cells using p90RSK(Ab-352) Antibody.
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90RSK(Ab-352) Antibody(left) or the same antibody preincubated with blocking peptide(right).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) RPS6KA1 Polyclonal antibody
  • Uniprot No.：
    Q15418
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Peptide sequence around aa.350~354 (R-D-S-P-G) derived from Human p90RSK.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB. Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration.
  • 基因功能参考文献：
    1. FASN-induced S6 kinase facilitates USP11-eIF4B complex formation for sustained oncogenic translation in diffuse large B-cell lymphoma. PMID: 29483509
    2. Polymorphism in p90Rsk gene is associated with Fetal Alcohol Spectrum Disorders. PMID: 29109170
    3. The results suggested a possible link between tRNALeu overexpression and RSK1/MSK2 activation and ErbB2/ErbB3 signaling, especially in breast cancer. PMID: 28816616
    4. Phosphorylation at Ser732 affects ribosomal S6 kinase 1 (RSK1) C-terminal tail (CTT) binding. PMID: 29083550
    5. RSK1 induced self-ubiquitination and destabilisation of UBE2R1 by phosphorylation but did not phosphorylate FBXO15. PMID: 27786305
    6. Genetic or pharmacologic inhibition of p90RSK in ganetespib-resistant cells restored sensitivity to ganetespib, whereas p90RSK overexpression induced ganetespib resistance in naive cells, validating p90RSK as a mediator of resistance and a novel therapeutic target PMID: 28167505
    7. These results suggest that RSK1 protects P-gp against ubiquitination by reducing UBE2R1 stability. PMID: 27786305
    8. Data suggest that UBR5 down-regulates levels of TRAF3, a key component of Toll-like receptor signaling, via the miRNA pathway; p90RSK is an upstream regulator of UBR5; p90RSK phosphorylates UBR5 as required for translational repression of TRAF3 mRNA. (UBR5 = ubiquitin protein ligase E3 component n-recognin 5 protein; TRAF3 = TNF receptor-associated factor 3; p90RSK = 90 kDa ribosomal protein S6 kinase) PMID: 28559278
    9. Data indicate that BTG2, MAP3K11, RPS6KA1 and PRDM1 as putative targets of microRNA miR-125b. PMID: 27613090
    10. The p90RSK has an essential role in promoting tumor growth and proliferation in non-small cell lung cancer (NSCLC). BID may serve as an alternative cancer treatment in NSCLC. PMID: 27236820
    11. RSK1 binds to EBP50 at its first PDZ domain, and mitogen activated RSK1 phosphorylates EBP50 at T156, an event that is crucial for its nuclear localization PMID: 26862730
    12. Data show that the 90 kDa ribosomal protein S6 kinases RSK1 and RSK2 play a key role in the homing of ovarian cancer cells in metastatic sites by regulating cell adhesion and invasion. PMID: 26625210
    13. RSK1 and 3 but not RSK2 are down-regulated in breast tumour and are associated with disease progression. RSK may be a key component in the progression and metastasis of breast cancer. PMID: 26977024
    14. PKD2 and RSK1 regulate integrin beta4 phosphorylation at threonine 1736 to stabilize keratinocyte cell adhesion and its hemidesmosomes. PMID: 26580203
    15. Results indicate that the phosphorylation of EphA2 at Ser-897 is controlled by RSK and the RSK-EphA2 axis might contribute to cell motility and promote tumour malignant progression. PMID: 26158630
    16. SL0101 and BI-D1870 induce distinct off-target effects in mTORC1-p70S6K signaling, and thus, the functions previously ascribed to RSK1/2 based on these inhibitors should be reassessed. PMID: 25889895
    17. RSK1 was constitutively phosphorylated at Ser-380 in nodular but not superficial spreading melanoma and did not directly correlate with BRAF or MEK activation. RSK1 orchestrated a program of gene expression that promoted cell motility and invasion. PMID: 25579842
    18. p90RSK-mediated SENP2-T368 phosphorylation is a master switch in disturbed-flow-induced signaling. PMID: 25689261
    19. These results suggest a critical role for ORF45-mediated p90 Ribosomal S6 Kinase activation in Kaposi's sarcoma-associated herpesvirus lytic replication. PMID: 25320298
    20. Data suggest that the ribosomal S6 kinase : protein kinase B (AKT) phosphorylation ratio could be useful as a biomarker of target inhibition by RAD001. PMID: 24332215
    21. RSK1 is specifically required for cleavage furrow formation and ingression during cytokinesis. PMID: 24269382
    22. RSK-mediated phosphorylation is required for KIBRA binding to RSK1. PMID: 24269383
    23. Data indicate that the S100B-p90 ribosomal S6 kinase (RSK) complex was found to be Ca2+-dependent, block phosphorylation of RSK at Thr-573, and sequester RSK to the cytosol. PMID: 24627490
    24. RSK1 is a novel regulator of insulin signaling and glucose metabolism and a potential mediator of insulin resistance, notably through the negative phosphorylation of IRS-1 on Ser-1101. PMID: 24036112
    25. Resistance to trastuzumab was observed in tumor cells with elevated MNK1 expression, furthermore, inhibition of RSK1 restored sensitivity to resistant cells. PMID: 22249268
    26. Targeting p90 ribosomal S6 kinase eliminates tumor-initiating cells by inactivating Y-box binding protein-1 in triple-negative breast cancers. PMID: 22674792
    27. results suggest p90 RSK facilitates nuclear Chk1 accumulation through Chk1-Ser-280 phosphorylation and that this pathway plays an important role in the preparation for monitoring genetic stability during cell proliferation. PMID: 22357623
    28. structure indicates that activation of RSK1 involves the removal of alpha-helix from the substrate-binding groove induced by ERK1/2 phosphorylation PMID: 22683790
    29. Data indicate that Plk1 siRNA interference and overexpression increased phosphorylation of RSK1, suggesting that Plk1 inhibits RSK1. PMID: 22427657
    30. melatonin enhances cisplatin-induced apoptosis via the inactivation of ERK/p90RSK/HSP27 cascade PMID: 22050627
    31. Collectively, these results identify a novel locus of apoptosomal regulation wherein MAPK signalling promotes Rsk-catalysed Apaf-1 phosphorylation and consequent binding of 14-3-3varepsilon, resulting in decreased cellular responsiveness to cytochrome c. PMID: 22246185
    32. Type I keratin 17 protein is phosphorylated on serine 44 by p90 ribosomal protein S6 kinase 1 (RSK1) in a growth- and stress-dependent fashion PMID: 22006917
    33. the results highlight a novel role for RSK1/2 and HSP27 phosphoproteins in P. aeruginosa-dependent induction of transcription of the IL-8 gene in human bronchial epithelial cells. PMID: 22031759
    34. Data show that VASP and Mena interact with RSK1. PMID: 21423205
    35. Data show that SH3P2 was phosphorylated on Ser(202) by ribosomal S6 kinase (RSK) in an ERK pathway-dependent manner, and such phosphorylation inhibited the ability of SH3P2 to suppress cell motility. PMID: 21501342
    36. our data provide evidence for a critical role for the activated RSK1 in IFNlambda signaling PMID: 21075852
    37. Data show that genetic variation in RPS6KA1, RPS6KA2, and PRS6KB2 were associated with risk of developing colon cancer while only genetic variation in RPS6KA2 was associated with altering risk of rectal cancer. PMID: 21035469
    38. small molecules such as celecoxib induce DR5 expression through activating ERK/RSK signaling and subsequent Elk1 activation and ATF4-dependent CHOP induction PMID: 21044953
    39. p22(phox)-based Nox oxidases maintain HIF-2alpha protein expression through inactivation of tuberin and downstream activation of ribosomal protein S6 kinase 1/4E-BP1 pathway PMID: 20304964
    40. was found to be activated by lead in a PKC- and MAPK-dependent manner PMID: 11861786
    41. Regulation of an activated S6 kinase 1 variant reveals a novel mammalian target of rapamycin phosphorylation site. PMID: 11914378
    42. TF cytoplasmic domain-independent stimulation of protein synthesis via activation of S6 kinase contributes to FVIIa effects in pathophysiology. PMID: 12019261
    43. activated transiently by stromal cell-derived factor 1 alpha alone or synergistically in combination with other cytokines PMID: 12036856
    44. Mammalian cell size is controlled by mTOR and its downstream targets S6K1 and 4EBP1/eIF4E PMID: 12080086
    45. RSK1 is negatively regulated by 14-3-3beta PMID: 12618428
    46. overexpressed in breast tumors PMID: 15112576
    47. Results suggest that active fibroblast growth factor receptor 1 kinase regulates the functions of nuclear 90-kDa ribosomal S6 kinase. PMID: 15117958
    48. that p90 ribosomal S 6 protein kinase 1 (RSK1) mediates the PGE2-induced phosphorylation of cAMP-response element binding protein PMID: 15615708
    49. monitored 14 previously uncharacterized and six known phosphorylation events after phorbol ester stimulation in the ERK/p90 ribosomal S6 kinase-signaling targets, TSC1 and TSC2, and a protein kinase C-dependent pathway to TSC2 phosphorylation PMID: 15647351
    50. S6 kinase 1 is a novel mammalian target of rapamycin (mTOR)-phosphorylating kinase PMID: 15905173

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, S6 kinase subfamily
  • 数据库链接：

    HGNC: 10430

    OMIM: 601684

    KEGG: hsa:6195

    STRING: 9606.ENSP00000435412

    UniGene: Hs.149957