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Raf1 Antibody

  • 货号：
    CSB-PA950764
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extract from mouse brain tissue and C6 cells using Raf-1 Antibody
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Rattus norvegicus (Rat) Raf1 Polyclonal antibody
  • Uniprot No.：
    P11345
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Rat
  • 免疫原：
    Peptide sequence around aa. 641-645(T-S-P-R-L ) derived from Rat Raf-1.
  • 免疫原种属：
    Rattus norvegicus (Rat)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. Phosphorylates TNNT2/cardiac muscle troponin T.
  • 基因功能参考文献：
    1. Wild-type PKGII phosphorylated Raf-1 at Ser-43 in a cGMP-dependent manner, but a PKGII D412A/R415A mutant, which has a low affinity for cGMP, did not. PMID: 28057484
    2. Raf-1 kinase contributes to cerebral vasospasm and early brain injury after subarachnoid hemorrhage by enhancing the activation of the Raf-1/ERK1/2 and Raf-1/NF-kappaB signaling pathways. PMID: 25367879
    3. sorafenib exhibits a regulatory role on the occurrence of LVH following AB in rats by blocking the rise in growth factors PDGF-BB and TGFbeta1, activation of the corresponding c-Raf-ERK1/2 signaling pathway PMID: 24885948
    4. Our results demonstrate that XIAP plays a new role as a negative regulator of neurotrophin-induced neurite outgrowth and neuronal differentiation in developing neurons. PMID: 23928917
    5. Induction of streptozotocin diabetes resulted in decreased level of GLP-1R and increased levels of IRS-1 and Raf-1 in the liver. PMID: 22983684
    6. Data show that a dimeric Raf kinase inhibitor protein (RKIP) mutant displayed a higher affinity to G protein-coupled receptor kinase (GRK) 2, but a lower affinity to Raf1. PMID: 22610096
    7. Protein arginine methyltransferase 5 regulates ERK1/2 signal transduction amplitude and cell fate through CRAF. PMID: 21917714
    8. RAF1 mutants caused hypertrophy signaled via nuclear factor of activated T cells PMID: 21440552
    9. These results indicate the initial robust activation of ERK phosphorylates Raf-1 at Ser289/296/301, resulting in Raf-1inhibition and then prompt inactivation of ERK following sublethal preconditioning ischemia. PMID: 20953701
    10. Raf-1 played a significant role in sustained morphine-mediated paradoxical pain sensitization and antinociceptive tolerance in vivo. PMID: 20718739
    11. Raf-1 kinase plays an important role in the regulation of vascular contractility through regulation of calcium sensitization. PMID: 20110729
    12. the role of Raf-1 in v-Ha-ras transformation PMID: 11933072
    13. Affinity with Raf is sufficient for Ras to efficiently induce rat mammary carcinomas PMID: 12538354
    14. Raf-1 has a role in modulating integrin-stimulated ERK activation when bound to CAMKII PMID: 12954639
    15. Changes in flexibility upon protein-protein complex formation of H-Ras & the Ras-binding domain of C-Raf1 have been investigated using the molecular framework approach FIRST and molecular dynamics simulations of in total approximately 35 ns length. PMID: 15211515
    16. selective modulation of c-Raf-1 but not B-Raf activation by RKIP can limit the dynamic range of the MAPK signaling response to growth factors and may play a critical role in growth and development PMID: 15886202
    17. aldosterone seems to exert mainly MR-induced effects that stimulate c-Raf, MEK1/2, MAPK1/2, the activities of CDK2 and CDK4, and the cell-cycle progression in mesangial cells PMID: 15975997
    18. c-raf.1 expression was located in the late basophilic lesions associated with hepatocellular carcinoma. PMID: 17554210
    19. Our results suggest that ROS production mediates the biphasic activation of ERK through different signaling cascades following post-ischemic reperfusion. Mediation of these signaling pathways involves changes in Raf-1 phosphorylation at different sites. PMID: 18243549
    20. AhR is a master regulator of c-raf and propose cross-talk between AhR and the mitogen-activated protein kinase signaling pathway. PMID: 18708364
    21. These studies identify Raf-dependent cTnT-Thr(206) phosphorylation as a novel mechanism that would link growth factor-dependent signaling pathways to dynamic changes in cardiac contractile function. PMID: 19381846

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  • 亚细胞定位：
    Cytoplasm. Cell membrane. Mitochondrion. Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily
  • 数据库链接：

    KEGG: rno:24703

    STRING: 10116.ENSRNOP00000013831

    UniGene: Rn.33262