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SF1 (Ab-82) Antibody

  • 货号：
    CSB-PA963117
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from COLO205 cells, using SF1 (Ab-82) antibody.
    • Immunohistochemistry analysis of paraffin-embedded human brain tissue using SF1 (Ab-82) antibody.
    • Immunofluorescence analysis of HeLa cells, using SF1 (Ab-82) antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) SF1 Polyclonal antibody
  • Uniprot No.：
    Q15637
  • 基因名：
    SF1
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized non-phosphopeptide derived from Human SF1 around the phosphorylation site of serine 82 (S-P-S(p)-P-E).
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC,IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:100
    IF 1:100-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.
  • 基因功能参考文献：
    1. SF1 Phosphorylation Enhances Specific Binding to U2AF(65) and Reduces Binding to 3'-Splice-Site RNA PMID: 28002734
    2. A novel function of SF1 in the initial recruitment of the U2 snRNP through direct interactions with two U2 snRNP-associated proteins. PMID: 26420826
    3. Data suggest that post-translational processing of SF1 (phosphorylation of Ser20) down-regulates nuclear import of SF1 via alterations in kinetic interaction of SF1 nuclear localization signal (NLS) with NLS receptor isoforms. PMID: 25043850
    4. We demonstrated that PRPF40B interacts directly with SF1 and associates with U2AF(65 PMID: 25605964
    5. Gomafu indirectly modulates the function of the splicing factors SF1 and Celf3 by sequestering these proteins into separate nuclear bodies. PMID: 25145264
    6. The conserved SPSP motif phosphorylation and the SF1/U2AF interface are essential in vivo. PMID: 23273425
    7. Findings suggest that Zinc finger motif-1 (ZFM1) is an important factor for the stabilization of a contractile SMC phenotype under basal or mildly activating conditions. PMID: 22906951
    8. central ;mystery' domain of SF1 crystals belonged to space group C2 and have most probable solvent contents of 64, 52 or 39% with three, four or five molecules per asymmetric unit, respectively PMID: 21505248
    9. SF1 silencing affected alternative splicing of endogenous transcripts, establishing a previously unexpected role for SF1 and branch site-like sequences in splice site selection. PMID: 21062807
    10. the conformational changes that are induced by assembly of the SF1/U2AF(65)/RNA complex serve to position the pre-mRNA splice site optimally for subsequent stages of splicing. PMID: 21146534
    11. SF3a60, 66, and 120, but not SF1, are essential for pre-mRNA splicing PMID: 15647371
    12. SF1 was essential for the induction of alternative mRNA splicing by the beta-catenin/TCF4 complex. PMID: 17383426
    13. Puf60-UHM binds to ULM sequences in the splicing factors SF1, U2AF65, and SF3b155. PMID: 18974054
    14. The KH-QUA2 region of SF1 defines an enlarged KH (hn RNP K) fold which is necessary and sufficient for intron branched point sequence (BPS) binding. PMID: 11691992

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    BBP/SF1 family
  • 组织特异性：
    Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.
  • 数据库链接：

    HGNC: 12950

    OMIM: 601516

    KEGG: hsa:7536

    STRING: 9606.ENSP00000366604

    UniGene: Hs.502829