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SLC12A2 Antibody

  • 货号：
    CSB-PA021383LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate,A549 whole cell lysate,JK whole cell lysate
      All lanes: SLC12A2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 132 kDa
      Observed band size: 132 kDa
    • Immunofluorescence staining of Hela cell with CSB-PA021383LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of A549 cell with CSB-PA021383LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    The SLC12A2 polyclonal antibody is obtained from rabbits with the recombinant human SLC12A2 protein (110-282aa) immunized. It is specific for human and mouse SLC12A2 proteins. This SLC12A2 antibody underwent protein G affinity and got at least 95% purity. The SLC12A2 antibody was validated for use in ELISA, WB, IHC, and IF applications.

    The SLC12A2 protein is particularly important in the kidneys, where it is involved in the reabsorption of ions and water from urine. SLC12A2 has also been implicated in other cellular processes, including cell proliferation, differentiation, and migration. Mutations in the SLC12A2 gene can lead to different pathological conditions, including hypertension, epilepsy, and cancer.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) SLC12A2 Polyclonal antibody
  • Uniprot No.：
    P55011
  • 基因名：
    SLC12A2
  • 别名：
    Basolateral Na-K-Cl symporter antibody; BSC antibody; BSC2 antibody; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 antibody; mBSC2 antibody; MGC104233 antibody; NKCC1 antibody; PPP1R141 antibody; Protein phosphatase 1; regulatory subunit 141 antibody; S12A2_HUMAN antibody; Slc12a2 antibody; sodium-potassium-chloride cotransporter 1 antibody; Solute carrier family 12 (sodium/potassium/chloride transporter); member 2 antibody; solute carrier family 12 (sodium/potassium/chloride transporters) member 2 antibody; Solute carrier family 12 member 2 antibody; sy-ns antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Solute carrier family 12 member 2 protein
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，SLC12A2 Antibody (CSB-PA021383LA01HU)，的标记方式是Non-conjugated。对于SLC12A2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA021383LB01HU SLC12A2 Antibody, HRP conjugated ELISA
    FITC CSB-PA021383LC01HU SLC12A2 Antibody, FITC conjugated
    Biotin CSB-PA021383LD01HU SLC12A2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative:  0.02% sodium azide
    Constituents:PBS containing 50% glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IF 1:30-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Cation-chloride cotransporter which mediates the electroneutral transport of chloride, potassium and/or sodium ions across the membrane. Plays a vital role in the regulation of ionic balance and cell volume.
  • 基因功能参考文献：
    1. NKCC1 not only controls cell volume and Cl- concentration, but it can also regulate the actin cytoskeleton through Cofilin 1. PMID: 28679472
    2. This new functional assay offered a robust working model for NKCC1 in determining reliable and concordant rank orders of the test compounds supporting its sensitivity and specificity. PMID: 28631939
    3. we introduce the molecular mechanism by which flavonoids, specifically quercetin, act through elevation of [Cl(-) ]c via activation of NKCC1 on important factors controlling various body and cellular functions, such as (1) antihypertensive actions controlling blood volume dependent on the amounts of renal Na(+) reabsorption via expression of the epithelial Na(+) channel PMID: 28574574
    4. NKCC1 and KCCs are coordinately regulated by L-WNK1 isoforms. PMID: 27170636
    5. NKCC1 high expression predicted a bad clinical outcome for lung adenocarcinoma patients and EGFR-mutated subgroup. Therefore, NKCC1 may play a role in lung adenocarcinoma and novel therapeutic tactics could be developed by targeting NKCC1 protein. PMID: 26559081
    6. Study describes a functional missense variant in SLC12A2 in human schizophrenia, and suggest that genetically encoded dysregulation of NKCC1 may be a risk factor for, or contribute to the pathogenesis of, schizophrenia PMID: 26955005
    7. The NKCC-1 is a Na(+)- dependent Cl(-) transporter that mediates the movement of Na(+), K(+), and Cl(-) ions across the plasma membrane and maintains cell volume and intracellular K(+) and Cl(-) homeostasis. PMID: 25620102
    8. NKCC1 labeling was seen only in the basolateral membrane of the secretory coils. PMID: 25218052
    9. Suggest that the expression of NKCC1 in esophageal squamous cell carcinoma may affect the G2/M checkpoint and may be related to the degree of histological differentiation of SCCs. PMID: 24944475
    10. Functional expression of human NKCC1 from a synthetic cassette-based cDNA: introduction of extracellular epitope tags and removal of cysteines. PMID: 24339991
    11. our data show a novel role for the WNK1/OSR1/NKCC1 pathway in glioma migration PMID: 24555568
    12. NKCC activation involves movement of TM12 relative to TM10, which is likely tied to movement of the large C terminus PMID: 24451383
    13. Reduced KCC2/NKCC1 ratio in the cerebrospinal fluid of Rett Syndrome patients suggests a disturbed process of GABAergic neuronal maturation and open up a new therapeutic perspective. PMID: 23894354
    14. The hormone aldosterone was found to upregulate NKCC1 by increasing protein stability. PMID: 24173102
    15. The rs10089 single nucleotide polymorphism was associated with increased susceptibility to noise-induced hearing loss. PMID: 23224734
    16. Altered hippocampal area function and coupling in DISC1 and SLC12A2 minor allele carriers. PMID: 23921125
    17. The results indicate a role for COMMD1 in the regulation of NKCC1 membrane expression and ubiquitination. PMID: 23515529
    18. These findings suggest that NKCC1 and AQP1 participate in meningioma biology and invasion PMID: 23317544
    19. A significant association is found between single nucleotide polymophisms in SLC12A2 and CTXN3 and schizophrenia in a Thai population. PMID: 22643131
    20. Capsaicin inhibits chloride secretion in part by causing NKCC1 internalization, but by a mechanism that appears to be independent of TRPV1. PMID: 23139219
    21. NKCC1 deficiency increases the size of focal adhesions. PMID: 22570591
    22. predictions of homology models of NKCC1 and demonstrate important roles for TM3 residues in ion translocation and loop diuretic inhibition. PMID: 22437837
    23. NKCC1 plays an important and yet partial role in RVI in C-20/A4 chondrocytes. PMID: 21847667
    24. The coupling between salt and water transport in NKCC1 represents a novel aspect of cellular water homeostasis where cells can change their volume independently of the direction of an osmotic gradient across the membrane. PMID: 20819947
    25. phosphorylation-induced activation of NKCC1 by osmotic shrinkage does not involve AMP-activated protein kinase and is likely to be due to STE20/SPS1-related proline/alanine-rich kinase activation PMID: 20442269
    26. The result points at secretion as the main mechanism of cyst filling, and NKCC1 as the key candidate of fluid transport. PMID: 20471979
    27. decreased NKCC1 may contribute to the feature of the pathogenesis of salt-sensitive hypertension seen in African Americans. PMID: 20044742
    28. NKCC1 modulates blood pressure through vascular and renal effects--REVIEW PMID: 20061948
    29. NKCC1 mRNA is expressed predominately in small- and medium-diameter primary afferent neurons,21 and the NKCC1 population of sensory neurons includes a large proportion that express TrpV1 and thus are presumably capsaicin-sensitive nociceptorscu PMID: 19916249
    30. NKCC1 was found in human HuH-7 hepatoma cells. The data suggest a role of NKCC1 in stellate cell transformation, hepatic volume regulation, and long-term adaption to dehydrating conditions. PMID: 12054469
    31. most CFTR-positive ADPKD cysts also express NKCC1 suggests that transepithelial Cl(-) secretion in ADPKD involves molecular mechanisms similar to secretory epithelia. PMID: 12355171
    32. all of the CCCs examined (NKCC1, NKCC2, KCC1, KCC3, and KCC4) can promote NH4(+) translocation, presumably through binding of the ion at the K(+) site PMID: 12657561
    33. data conclusively link PASK with the phosphorylation and activation of NKCC1 PMID: 12740379
    34. the stimulation of either luminal or basolateral P2R increased NKCC1 activity, which was observed in the basolateral membrane, but not in the luminal membrane PMID: 14982922
    35. the uncovered interacting domains are probably a major determinant of the NKCC1 conformational landscape PMID: 15280386
    36. Data indicate that binding of hsp90 to the Na(+)-K(+)-Cl(-) cotransporter (NKCC1) may be required for sodium-potassium-chloride cotransport to occur at the cell surface. PMID: 15347682
    37. role of the complex of serine/threonine protein kinases and a protein phosphatase is probably the maintenance of optimal phosphorylation of NKCC1 coincident with its physiological function in epithelial absorption and secretion PMID: 15899883
    38. Taken together, the results of the present study indicate that the signal transduction protein, controlled by the Na+/K+/Cl- cotransporter, must be downstream of the PKC, and at/or upstream to MEK in the Ras/Raf/MEK/ERK cascade. PMID: 16222701
    39. NKCC1 transporter facilitates seizures in the developing brain. PMID: 16227993
    40. persistent NKCC1 activation by cAMP is constrained by a Ca(2+)-dependent cycle of co-transporter internalization, degradation and re-expression; this is a novel mechanism to limit intestinal fluid loss. PMID: 17478539
    41. Ammonium was transported on NKCC1 in T84 cells nearly as well as potassium PMID: 18032481
    42. Reveal a novel role for the EGFR in the chronic regulation of epithelial secretory capacity through upregulation of NKCC1 expression. PMID: 18400987
    43. membrane rafts render KCC2 inactive and NKCC1 active PMID: 19686239

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  • 亚细胞定位：
    Basolateral cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    SLC12A transporter family
  • 组织特异性：
    Expressed in many tissues.
  • 数据库链接：

    HGNC: 10911

    OMIM: 600840

    KEGG: hsa:6558

    STRING: 9606.ENSP00000262461

    UniGene: Hs.162585