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SLC28A3 Antibody

  • 货号：
    CSB-PA884504LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA884504LA01HU diluted at 1:200 and staining in paraffin-embedded human Prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37℃ Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of HepG2 cells with CSB-PA884504LA01HU at 1:200, counter-stained with DAPI.The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 其他：

产品详情

  • 产品描述：

    CUSABIO immunizes the rabbit with a partial protein mapping within the topological domain of the human SLC28A3 protein to generate the anti-SLC28A3 antibody. This SLC28A3 polyclonal antibody exhibits as the unconjugated IgG isoform. Its purity is 95%+ using protein G purified. It shows reactivity with human and mouse samples. And it has been validated to recognize SLC28A3 protein in ELISA, IHC, and IF applications. Its target protein SLC28A3 is an important mediator of the cellular entry of a number of physiological nucleosides and synthetic anticancer nucleoside analog drugs.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) SLC28A3 Polyclonal antibody
  • Uniprot No.：
    Q9HAS3
  • 基因名：
    SLC28A3
  • 别名：
    SLC28A3 antibody; CNT3Solute carrier family 28 member 3 antibody; Concentrative Na(+)-nucleoside cotransporter 3 antibody; CNT 3 antibody; hCNT3 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Solute carrier family 28 member 3 protein (1-102AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，SLC28A3 Antibody (CSB-PA884504LA01HU)，的标记方式是Non-conjugated。对于SLC28A3 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA884504LB01HU SLC28A3 Antibody, HRP conjugated ELISA
    FITC CSB-PA884504LC01HU SLC28A3 Antibody, FITC conjugated
    Biotin CSB-PA884504LD01HU SLC28A3 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Sodium-dependent, pyrimidine- and purine-selective. Involved in the homeostasis of endogenous nucleosides. Exhibits the transport characteristics of the nucleoside transport system cib or N3 subtype (N3/cib) (with marked transport of both thymidine and inosine). Employs a 2:1 sodium/nucleoside ratio. Also able to transport gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine.
  • 基因功能参考文献：
    1. De novo structure prediction of three N-terminal transmembrane helices of the human concentrative nucleoside transporter 3 (hCNT3) homotrimer belonging to the solute carrier 28 family of transporters (SLC28) using Rosetta program and its Broker protocol. PMID: 28774292
    2. CYR61 negatively regulates the nucleoside transporters hENT1 and hCNT3 in pancreatic ductal adenocarcinoma. PMID: 27604902
    3. Data suggest that CNT3 forms a homotrimer in solution and membrane-bound inside cells; the quaternary structure creates an aqueous basin that significantly shortens the substrate translocation distance. PMID: 28661652
    4. results that validate the newly developed structural homology model of CNT membrane architecture for human CNTs, revealed extended conformationally mobile regions within transport-domain TMs, identified pore-lining residues of functional importance, and provided evidence of an emerging novel elevator-type mechanism of transporter function. PMID: 28385889
    5. Genome-wide association analysis on normal hearing function identifies PCDH20 and SLC28A3 as good candidates for modulatory genes in the auditory system. [meta-analysis] PMID: 26188009
    6. The co-expression of galectin-4 and CNT3 proteins is not impaired in inflamed colon from patients with Crohn's disease, thereby anticipating the integrity of this system for drug targeting. PMID: 26481311
    7. Results show that high CNT3 expression level is associated with overall favorable outcomes and is predictive of clinical outcomes in acute myeloid leukemia patients with t(8;21). PMID: 25955569
    8. The presence of homozygous major allele for SLC28A3 (CC genotype) were each associated with an almost two-fold increase in the formation clearance of dFdCTP. PMID: 24300978
    9. Genetic polymorphisms in SLC28A3, SLC29A1 and RRM1 can influence the clinical outcome of metastatic breast cancer patients treated with paclitaxel-gemcitabine chemotherapy. PMID: 24361227
    10. association between ribavirin (RBV) serum levels and SLC28A2 rs11854484 genotype, as well as the replicated association of ITPA and SLC28A3 genetic polymorphisms with RBV-induced anemia and treatment response PMID: 23195617
    11. Data show that ENT1, ENT2, ENT4 and CNT3 protein was detected on ovarian carcinoma cells in all effusions, with expression observed in 1-95% of tumor cells. PMID: 21822668
    12. A genetic variant in SCL28A3 coding for the concentrative nucleoside transporter 3 protects patients with chronic hepatitis C against hemolytic anemia without affecting sustained virological response in hepatitis C virus genotype 1. PMID: 21346688
    13. Acidic and hydrophobic motifs in the N terminus tail of the hCNT3 control ER export and cell surface expression levels in nonpolarized cells, whereas a putative beta-turn domain contributes to hCNT3 polarized surface expression in epithelial cells. PMID: 20643903
    14. H+ drives uridine and adenosine transport by hCNT3 with lower affinity but higher maximal transport rate than Na+. PMID: 20495821
    15. TGF-beta1 acts through activation of ERK1/2 and the small GTPase RhoA to promote plasma membrane trafficking of the hCNT3 protein. PMID: 20172853
    16. hCNT3 gene is evolutionarily conserved with hCNT1 and hCNT2. Physiologically, hCNT3 is a glycoprotein, which transports purine and pyrimidine nucleosides in a Na-dependent manner with high affinities. PMID: 14504928
    17. genetic analysis and functional characterization of CNT3 variants suggest that this transporter does not tolerate nonsynonymous changes and is important for human fitness PMID: 15738947
    18. examination of single nucleotide polymorphisms in the coding regions of the hCNT3 gene PMID: 15861042
    19. hCNT3 possesses two Na+-binding sites, only one of which is shared by H+ PMID: 15870078
    20. The effects of cysteine substitution mutants spanning transmembrane domains 11-13 on the transport activity of CNT3 expressed in S. cerevisiae are reported. PMID: 16271041
    21. Minimal features required for human CNT3 transport are two hydrogen bond acceptors at 3'-OH and 5'-O and the hydrophobic center occupied by the base ring. PMID: 16446384
    22. transcripts for CNT3 protein in human kidneys and in cultured proximal tubule cells suggest involvement of CNT3 in renal handling of nucleosides and nucleoside drugs PMID: 17409283
    23. CNT3 was inserted into the apical membrane, thus generating, a transepithelial flux of both nucleosides and nucleoside-derived drugs. PMID: 17412768
    24. hCNT2 shares common cation specificity and coupling characteristics with hCNT1, which differ markedly from those of hCNT3. PMID: 17453413
    25. This study shows that adenosine elimination on human airway epithelia is mediated by ADA1, CNT2, and CNT3, which constitute important regulators of adenosine-mediated inflammation. PMID: 17696452
    26. These data suggest that selected genes of the SLC28 and SLC29 families are not only targets of HIV-1 infection, but might also contribute to the development of adipose tissue alterations leading to lipodystrophy. PMID: 17926640
    27. A polymorphic variant of the human concentrative nucleoside transporter, CNT3, is found that severely affects its functionality. PMID: 17993510
    28. identified a C-terminal intramembranous cysteine residue of hCNT3 (Cys-561) that reversibly binds the hydrophilic thiol-reactive reagent p-chloromercuribenzene sulfonate (PCMBS) PMID: 18199742
    29. a pivotal functional role for Cys-561 in Na+- as well as H+-coupled modes of hCNT3 nucleoside transport PMID: 18621735
    30. The isolation and charcterization of an alternatively spliced SLC28A3-related mRNA is reported. PMID: 18827020
    31. This evidence suggested that apical CNT3 and basolateral ENT2 are involved in proximal tubular reabsorption of adenosine and some nucleoside drugs and that apical ENT1 is involved in proximal tubular secretion of 2'-deoxyadenosine. PMID: 19297449
    32. Pancreatic adenocarcinoma patients with a high expression of hENT1 and hCNT3 immunostaining have a significantly longer survival after adjuvant gemcitabine-based chemoradiation PMID: 19318496
    33. identified residues of functional importance and with a revised 15-TM membrane architecture, suggest a novel membrane-associated topology for a region of the protein (TM 11A) that includes the highly conserved CNT family motif (G/A)XKX(3)NEFVA(Y/M/F) PMID: 19380585
    34. identified two conserved pore-lining glutamate residues (Glu-343 and Glu-519) with essential roles in CNT3 Na(+)/nucleoside and H(+)/nucleoside cotransport PMID: 19380587

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  • 亚细胞定位：
    [Isoform 1]: Cell membrane; Multi-pass membrane protein.; [Isoform 2]: Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Concentrative nucleoside transporter (CNT) (TC 2.A.41) family
  • 组织特异性：
    Expressed in pancreas, bone marrow, trachea, mammary gland, liver, prostate, and regions of intestine, brain, lung, placenta, testis, kidney, and heart.
  • 数据库链接：

    HGNC: 16484

    OMIM: 608269

    KEGG: hsa:64078

    STRING: 9606.ENSP00000365413

    UniGene: Hs.535966