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SPINK5 Antibody

  • 货号：
    CSB-PA889077LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA889077LA01HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA889077LA01HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) SPINK5 Polyclonal antibody
  • Uniprot No.：
    Q9NQ38
  • 基因名：
  • 别名：
    Hemofiltrate peptide HF7665 antibody; ISK5_HUMAN antibody; LEKTI antibody; Lympho-epithelial Kazal-type-related inhibitor antibody; Lymphoepithelial Kazal type related inhibitor antibody; NETS antibody; NS antibody; Serine peptidase inhibitor Kazal type 5 antibody; SPINK5 antibody; VAKTI antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Serine protease inhibitor Kazal-type 5 protein (722-847AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，SPINK5 Antibody (CSB-PA889077LA01HU)，的标记方式是Non-conjugated。对于SPINK5 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA889077LB01HU SPINK5 Antibody, HRP conjugated ELISA
    FITC CSB-PA889077LC01HU SPINK5 Antibody, FITC conjugated
    Biotin CSB-PA889077LD01HU SPINK5 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense-activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin.
  • 基因功能参考文献：
    1. SPINK5 gene R654H might be a risk factor for epidermal barrier dysfunction in some Japanese AD patients. PMID: 29926005
    2. Early-onset atopic dermatitis was associated with rs2303067 in SPINK5, which is involved in skin barrier functioning, and late-onset was associated with rs4950928 in CHI3L1, which is involved in the immune response. Future studies should examine the early- versus late-onset subgrouping more closely PMID: 28681574
    3. report, for the first time, association between SPINK5 variant rs9325071 and challenge-proven IgE-mediated food allegy PMID: 28213955
    4. impaired KLK7 secretion from lamellar granules and increased LEKTI expression could underlie the insufficient activation of KLK in atopic dermatitis. PMID: 27769847
    5. This report discloses a prevalent SPINK5 founder mutation in Finland and illustrates Netherton syndrome phenotype variability PMID: 26865388
    6. New mutation leading to the full variety of typical features of the Netherton syndrome. PMID: 26031502
    7. The results suggest that SPINK5 and ADRB2 haplotypes might play a role in the susceptibility to childhood-onset asthma PMID: 25233048
    8. The effect of GATA3 on SPINK5 expression was indirect and GATA3 alone was insufficient for final differentiation of keratinocytes where full SPINK5 expression was observed. PMID: 24894987
    9. these results support epistasis between SPINK5 and TSLP, which contributes to childhood asthma. PMID: 24831437
    10. Netherton syndrome is caused by mutations in SPINK5, which encodes the serine protease inhibitor LEKTI. PMID: 24577329
    11. Herein we report three patients with Netherton syndrome who had growth retardation associated with GH deficiency and responded well to GH therapy. PMID: 24015757
    12. mesotrypsin contributes to the desquamation process by activating KLKs and degrading the intrinsic KLKs' inhibitor LEKTI PMID: 24390132
    13. The E420K LEKTI variant alters LEKTI proteolytic activation and results in protease deregulation. PMID: 22730493
    14. major binding partners of LEKTI were found to be the antimicrobial peptide dermcidin and the serine protease cathepsin G and no kallikreins. PMID: 22588119
    15. Our study represents the first identification of a Netherton disease-causing SPINK5 mutation that alters splicing without affecting canonical splice sites. PMID: 22377713
    16. the SPINK5 gene polymorphisms was found not to be associated with atopic dermatitis (AD) in regard to either serum IgE levels, concurrent allergic asthma or early onset of AD PMID: 21585560
    17. Lowered SPINK5 protein expression might be a contributing factor for the development of chronic rhinosinusitis. PMID: 22570283
    18. Distinct SPINK5 and IL-31 gene variants (SNPs) were associated with the development of atopic eczema and non-atopic hand dermatitis in Taiwanese nurses. PMID: 22017185
    19. New SPINK5 defects in 12 patients, who presented a clinical triad suggestive of Netherton syndrome with variations in inter- and intra-familial disease expression were disclosed. PMID: 22089833
    20. The novel mutation, p.Gln333X, in the SPINK5 gene, is responsible for a mild Netherton syndrome phenotype in a Turkish pedigree. PMID: 21564178
    21. Six SNPs (rs17718511, rs17860502, KN0001820, rs60978485, rs17718737, and rs1422985) and the haplotype TAA (rs60978485, rs6892205, rs2303064) in the SPINK5 gene were associated with atopic dermatitis in Koreans. PMID: 21087323
    22. Caspase 14 has been implicated as a novel target of LEKTI, which has the potential to act as both a serine and a cysteine protease inhibitor. PMID: 20533828
    23. study reports two male siblings affected by Netherton syndrome, which resulted from a previously undescribed splicing mutation in SPINK5 PMID: 20107740
    24. Highly significant associations were detected between SPINK5 single nucleotide polymorphisms and visible eczema (but not IgE levels) and between IL13 variants and total IgE. PMID: 20085599
    25. Even though the number of investigated subjects was small and hydrolytic activity was only slightly increased, the results denote that LEKTI might be diminished in atopic dermatitis PMID: 19522716
    26. SPINK5's role in atopic dermatitis and skin diseases is described PMID: 11796258
    27. the intron-exon organization of the gene and characterize the spink5 mutations. five mutations, one of which resulted in perinatal lethal disease, were associated with ethnic groups PMID: 11841556
    28. the defective inhibitory regulation of desquamation due to SPINK5 gene mutations may cause over-desquamation of corneocytes in Netherton syndrome, leading to severe skin permeability barrier dysfunction. PMID: 11874482
    29. This is a multidomain serine proteinase inhibitor with physiopathological significance. PMID: 11943586
    30. REVIEW: molecular cloning, characterization of the gene, tissue distribution, congenital disases associated with mutations PMID: 12437098
    31. LETKI proteolytic processing was studied in cultured keratinocytes as well as its tissue distribution and defective expression in Netherton syndrome. PMID: 12915442
    32. There is an association between polymorphisms in the SPINK5 gene and atopic dermatitis in the Japanese. PMID: 14551605
    33. LEKTI deficiency in the epidermis and in hair roots at the protein level and an aberrant expression of other proteins, especially transglutaminase1 and 3, which may account for the impaired epidermal barrier in Netherton syndrome PMID: 15304086
    34. The sequence from Leu32 to Ile38 of a chimeric double-mutant domain 1 of LEKTI is a chameleon sequence that converts a short 3(10)-helix into an extended loop conformation and parts of the COOH-terminal alpha-helix of domain 1 into a beta-hairpin. PMID: 15366933
    35. deficiency is related to epidermal detachment, desmosomal dissociation, and destabilization of corneodesmosin PMID: 15466487
    36. in normal skin the LG system transports and secretes LEKTI earlier than KLK7 and KLK5 preventing premature loss of stratum corneum integrity/cohesion. PMID: 15675955
    37. We showed that LEKTI is a potent inhibitor of a family of serine proteinases involved in extracellular matrix remodeling and its expression is downregulated in head and neck squamous cell carcinomas. PMID: 15680911
    38. Homozygous frameshift mutation in SPINK5 associated with Netherton syndrome. PMID: 15942217
    39. Variable amounts of SPINK5 alternative transcripts are detected in all SPINK5 transcriptionally active tissues. PMID: 16374478
    40. Mutations in SPINK5 encoding the serine protease (SP) inhibitor, lymphoepithelial-Kazal-type 5 inhibitor (LEKTI), cause Netherton syndrome (NS). SP activation correlated with clinical severity, and inversely with residual LEKTI expression. PMID: 16601670
    41. Uniparental disomy of maternal SPINK5 allele was indicated in the mutation analysis of two Taiwanese patients with Netherton syndrome. PMID: 17415575
    42. These results identify KLK5, a key actor of the desquamation process, as the major target of LEKTI. PMID: 17596512
    43. LEKTI domain 15 is a functional Kazal-type proteinase inhibitor. PMID: 17936012
    44. SPINK5 mutations, causing NS, lead to truncated LEKTI; each Netherton syndrome patient possesses LEKTI of a different length, depending on the location of mutations PMID: 17989726
    45. study in children and adults with atopic dermatitis found that an association with SPINK5 E420K SNP was not confirmed. However, this was associated with high IgE serum levels (p=0.011). PMID: 17989887
    46. SPINK5 (LEKTI) protein was detected in sinonasal tissue and was significantly decreased in polyp samples using IHC. PMID: 18588753
    47. SPINK5 mutation confers a risk of eczema when maternally inherited but is not a major eczema risk factor; no interaction between the SPINK5 risk allele or the putative KLK7 risk allele and FLG mutations ws found PMID: 18774391
    48. reduced expression of LEKTI and increased expression of SCCE and SCTE in human epidermal keratinocytes after UVB irradiation may contribute to desquamation of the stratum corneum. PMID: 19118981
    49. Haploinsufficiency of SPINK5 can cause the Netherton syndrome phenotype in the presence of one null mutation with homozygous G1258A polymorphisms in SPINK5, and this could impair the function of LEKTI and therefore acts as a true mutation. PMID: 19438860
    50. -206G>A polymorphism in the SPINK5 is associated with asthma susceptibility in a Chinese Han population PMID: 19534795

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  • 相关疾病：
    Netherton syndrome (NETH)
  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Highly expressed in the thymus and stratum corneum. Also found in the oral mucosa, parathyroid gland, Bartholin's glands, tonsils, and vaginal epithelium. Very low levels are detected in lung, kidney, and prostate.
  • 数据库链接：

    HGNC: 15464

    OMIM: 256500

    KEGG: hsa:11005

    STRING: 9606.ENSP00000352936

    UniGene: Hs.331555